PFI-TT: Development of a Single-Molecule Electronic Biosensor

PFI-TT：单分子电子生物传感器的开发

• 批准号: 1827671
• 负责人: Philip Collins
• 金额: $ 20万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2020-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827671&HistoricalAwards=false
• 关键词: PFI; TT; Development; Single; Molecule

The broader impact/commercial potential of this PFI project is to create a new type of biosensor for biomedical research. Biosensors are key tools in the study of how molecules bind to each other. Proteins, antibodies, peptides, DNA strands, and medicines all bind and release each other in ways that are critical for human health; and the study of these binding interactions is critical to finding new medicines, detecting diseases, and understanding our own biology. Better biosensors lead to better health outcomes, and the prototype biosensor developed by this project aims to reveal molecular interactions that are presently hidden to all other types of biosensors currently in use.The proposed project will achieve this goal using recent innovations in biosensing research. Specifically, electronic, solid-state biosensors have been successfully commercialized; and compatible single-molecule electronics have been proven to be useful for precision measurements of biochemical activity. The technical objective of this project will be a proof-of-concept hybrid device that combines conventional ensemble sensing side-by-side with complementary, electronic monitoring of single-molecule binding kinetics. This combination of simultaneous ensemble and single-molecule monitoring does not exist in any commercial sensor, but it provides new opportunities to break through fundamental limitations faced by all ensemble biosensors. Specifically, the project hypothesizes that the combined data will extend the biosensor's effective dynamic range and help eliminate false positive and false negative results. If successful, this type of hybrid sensor could establish a new paradigm where single-molecule data are leveraged in sensors generally to better inform end users in biology, healthcare, and manufacturing.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 PFI 项目更广泛的影响/商业潜力是为生物医学研究创建一种新型生物传感器。 生物传感器是研究分子如何相互结合的关键工具。 蛋白质、抗体、肽、DNA 链和药物都以对人类健康至关重要的方式相互结合和释放；对这些结合相互作用的研究对于寻找新药、检测疾病和了解我们自身的生物学至关重要。 更好的生物传感器可以带来更好的健康结果，该项目开发的原型生物传感器旨在揭示目前使用的所有其他类型的生物传感器所隐藏的分子相互作用。拟议的项目将利用生物传感研究的最新创新来实现这一目标。 具体来说，电子固态生物传感器已成功商业化；兼容的单分子电子器件已被证明可用于生化活性的精确测量。 该项目的技术目标将是一种概念验证混合设备，它将传统的整体传感与单分子结合动力学的互补电子监测结合起来。 这种同步整体和单分子监测的组合在任何商业传感器中都不存在，但它提供了突破所有整体生物传感器所面临的基本限制的新机会。 具体来说，该项目假设组合数据将扩展生物传感器的有效动态范围，并有助于消除假阳性和假阴性结果。 如果成功，这种类型的混合传感器可以建立一个新的范例，在传感器中普遍利用单分子数据，以更好地为生物学、医疗保健和制造领域的最终用户提供信息。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。