Genome-wide identification of regulators of morphogenetic variability in the human pathogenic yeast Candida albicans

人类致病酵母白色念珠菌形态发生变异调节因子的全基因组鉴定

• 批准号: 23937236
• 负责人: Professor Dr. Joachim Morschhäuser
• 金额: --
• 依托单位: Institut für Molekulare Infektionsbiologie (IMIB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/23937236?language=en
• 关键词: Genome; wide; identification; regulators; morphogenetic

Candida albicans is the most important human pathogenic fungus, but also a highly interesting organism with respect to its biology that exhibits many fascinating morphogenetic programs. These include the transition from yeast to filamentous growth, which is controlled by environmental signals, and the spontaneous phenotypic switching to a so-called opaque cell form, which is a requirement for mating between cells of different mating type. The morphological variability and corresponding changes in the gene expression pattern are also important for pathogenicity of this fungus, because they facilitate adaptation to different host niches. The regulation of the various developmental programs is only partially understood and many of the genes controlling them remain to be identified. The C. albicans genome has recently been sequenced, allowing a systematic functional analysis of all genes. As transcriptional activators and repressors play a central role in the regulation of phenotypic traits, the aim of this project is to analyze all transcription factors identified in the C. albicans genome for a possible function in the regulation of morphogenesis. Using a tetracycline-inducible gene expression system developed in our group, the effects of forced expression of each individual transcription factor in various growth conditions on yeast and hyphal growth, whiteopaque switching, and production of chlamydospores, the biological function of which still remains a mystery, will be investigated. This comprehensive analysis will contribute to a detailed understanding of the biology and pathogenicity of C. albicans.

白色念珠菌是最重要的人类致病真菌，但也是一个高度有趣的生物学方面，表现出许多迷人的形态发生程序。这些包括从酵母到丝状生长的转变，这是由环境信号控制的，以及自发的表型转换为所谓的不透明细胞形式，这是不同交配类型的细胞之间交配的要求。形态变异性和相应的基因表达模式的变化也是重要的致病性，这种真菌，因为它们有助于适应不同的主机生态位。各种发育程序的调节只是部分理解，许多控制它们的基因仍有待鉴定。梭白念珠菌基因组最近已被测序，允许所有基因的系统功能分析。由于转录激活因子和抑制因子在表型性状的调控中起着重要作用，本项目的目的是分析在C.白念珠菌基因组中的一个可能的功能，在调节形态发生。使用四环素诱导的基因表达系统，在我们的小组开发的，强制表达的每个单独的转录因子在各种生长条件下对酵母菌和菌丝生长，whiteopaque开关，厚垣孢子的生产，其生物学功能仍然是一个谜的影响，将进行调查。这一全面的分析将有助于详细了解C.白色念珠菌