Highly Sensitive Multiplexed Nanocone Array for Point-of-Care Pan-Cancer Screening

用于护理点泛癌症筛查的高灵敏度多重纳米锥阵列

• 批准号: 1931850
• 负责人: Tengfei Luo
• 金额: $ 38.14万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1931850&HistoricalAwards=false
• 关键词: Highly; Sensitive; Multiplexed; Nanocone; Array

Irregular amounts of specific molecules of micro-RNA (miRNA) in serum or plasma are promising biomarkers for many chronic diseases, particularly cancers. Different types of miRNAs have been found to be over-expressed for different cancers. The goal of this research is to develop optical fiber-based biosensors for point-of-care (POC) measurement of miRNA molecular biomarkers for a wide of range of bio-sensing applications. This research will leverage the team's previously developed pretreatment technologies for miRNA extraction from whole blood. To achieve POC early cancer screening, these pretreatment technologies will be integrated with the proposed sensor to achieve extremely high sensitivity of detection. In this sensor, specific probes attached to nanoparticles will be used to increase sensitivity. An integrated prototype for measuring multiple miRNAs will be delivered at end of this project, and this will be further developed to detect much larger libraries of miRNAs biomarkers in the future.The nanocone array is based on and fabricated with several intriguing and poorly understood physical phenomena at conic/wedge geometries. Like plasmonic resonance at metallic tips and scattering at dielectric wedges/cones, evanescent wave mode at a conic tip can produce a localized hotspot with high optical intensity. This tip evanescent mode is coupled to specific optical fiber wave-guide modes that undergoes multiple internal reflections. The resonant core-shell nanoparticles, which allows further plasmonic resonant enhancement, are immobilized around the tip by a unique laser bubble contact line deposition technique. The extreme curvature of the conic substrate controls the size of the laser-nucleated bubble on the cone tip and the subsequent contact-line receding rate. The deposition of the nanoparticle in the bulk solution to the wedge-like moving contact line is also controlled by singular heating and Marangoni effects at the wedge-like contact line. All these phenomena driven by the infinite curvatures of cones and wedges will be carefully studied combining computation and experiments. Within this proposed project, the team will design such a nanoarray for a promising set of cancer biomarkers, mi-RNAs, whose individual copy number ranges from 10^2 to 10^6 in a small-volume patient blood sample (~10 microliter). Leveraging their past experience and industrial connections, the PIs will seek commercialization opportunities for the proposed sensing platform. This project will also provide research opportunities to students from under-represented undergraduate groups, local community college and high school.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

血清或血浆中不规则数量的特定微rna分子（miRNA）是许多慢性疾病，特别是癌症的有希望的生物标志物。已经发现不同类型的mirna在不同的癌症中过度表达。本研究的目标是开发基于光纤的生物传感器，用于即时（POC）测量miRNA分子生物标志物，用于广泛的生物传感应用。这项研究将利用该团队之前开发的从全血中提取miRNA的预处理技术。为了实现POC早期癌症筛查，这些预处理技术将与所提出的传感器相结合，以实现极高的检测灵敏度。在这种传感器中，将使用附着在纳米颗粒上的特定探针来提高灵敏度。一个用于测量多个mirna的集成原型将在该项目结束时交付，并将在未来进一步开发以检测更大的mirna生物标记物库。纳米锥阵列是基于锥形/楔形几何形状的几种有趣而鲜为人知的物理现象而制造的。与金属尖端的等离子体共振和介质楔形/锥体的散射一样，锥体尖端的倏逝波模式可以产生高光强的局部热点。这种尖端倏逝模式与特定的光纤波导模式耦合，该模式经历多次内部反射。共振核壳纳米粒子通过独特的激光泡接触线沉积技术固定在尖端周围，从而进一步增强等离子体共振。圆锥基板的极端曲率控制着锥尖上激光成核气泡的大小和随后的接触线后退速率。纳米粒子在体溶液中向楔形移动接触线上的沉积也受到楔形接触线上的奇异加热和马兰戈尼效应的控制。所有这些由锥体和楔体无限曲率驱动的现象将结合计算和实验进行仔细研究。在这个提议的项目中，该团队将为一组有前途的癌症生物标志物设计这样的纳米阵列，mi- rna，其个体拷贝数在小容量患者血液样本（~10微升）中的范围从10^2到10^6。利用他们过去的经验和工业联系，pi将为拟议的传感平台寻求商业化机会。该项目还将为来自代表性不足的本科群体、当地社区大学和高中的学生提供研究机会。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Molecular-Level Understanding of Efficient Thermal Transport across the Silica–Water Interface

• DOI: 10.1021/acs.jpcc.1c06571
• 发表时间: 2021-10
• 期刊: The Journal of Physical Chemistry C
• 作者: Zhihao Xu;Dezhao Huang;T. Luo

Biocompatible Direct Deposition of Functionalized Nanoparticles Using Shrinking Surface Plasmonic Bubble

• DOI: 10.1002/admi.202000597
• 发表时间: 2020-06-01
• 期刊: ADVANCED MATERIALS INTERFACES
• 影响因子: 5.4
• 作者: Moon, Seunghyun;Zhang, Qiushi;Luo, Tengfei
• 通讯作者: Luo, Tengfei