RAPID: A rapid and ultrasensitive technology for sensing intact SARS-CoV-2 using designer DNA nanostructure capture probes and photonic resonator interference scattering microscopy

RAPID：一种快速、超灵敏的技术，使用设计的 DNA 纳米结构捕获探针和光子谐振器干涉散射显微镜来感测完整的 SARS-CoV-2

• 批准号: 2027778
• 负责人: Brian Cunningham
• 金额: $ 16.53万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027778&HistoricalAwards=false
• 关键词: RAPID; rapid; ultrasensitive; technology; sensing

The rapid development of the COVID-19 pandemic highlights the shortcomings in existing technologies for viral detection and identification. The lack of widespread availability of testing, combined with a high rate of false negative tests, contribute to quarantine failure and confusion among health authorities and the public. The investigator proposes to combine biosensing and biophotonics approaches to develop and demonstrate a rapid, room temperature, single-step, virus-specific, and ultrasensitive digital resolution assay for COVID-19 that can be performed immediately after sample collection at the point of care, and provide a result in less than 15 minutes. The investigator and his group recently demonstrated that a photonic crystal surface can amplify the scattering of light from surface-attached nanoparticles, including virions, and that a new form of biosensor microscopy called Photonic Resonator Interference Scattering Microscopy (PRISM) can detect and digitally count the virus particles in real time, without the use of additional labels or stains. These technologies will be rapidly adapted and tested for COVID-19 detection.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

COVID-19大流行的快速发展凸显了现有病毒检测和识别技术的不足。缺乏广泛的检测，再加上假阴性检测的高发生率，导致检疫失败，并使卫生当局和公众感到困惑。研究者建议将生物传感和生物光子学方法结合起来，开发和演示一种快速、室温、单步、病毒特异性和超灵敏的COVID-19数字分辨率检测方法，该方法可以在护理点采集样本后立即进行，并在不到15分钟内提供结果。研究人员和他的团队最近证明，光子晶体表面可以放大附着在表面的纳米颗粒（包括病毒粒子）的光散射，并且一种称为光子谐振器干涉散射显微镜（PRISM）的新型生物传感器显微镜可以实时检测和数字计数病毒颗粒，而无需使用额外的标签或染色。将对这些技术进行快速调整和测试，以检测COVID-19。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Label-Free Digital Detection of Intact Virions by Enhanced Scattering Microscopy.

• DOI: 10.1021/jacs.1c09579
• 发表时间: 2022-02-02
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Li, Nantao;Wang, Xiaojing;Tibbs, Joseph;Che, Congnyu;Peinetti, Ana Sol;Zhao, Bin;Liu, Leyang;Barya, Priyash;Cooper, Laura;Rong, Lijun;Wang, Xing;Lu, Yi;Cunningham, Brian T.
• 通讯作者: Cunningham, Brian T.

A photonic resonator interferometric scattering microscope for label-free detection of nanometer-scale objects with digital precision in point-of-use environments

光子谐振器干涉散射显微镜，用于在使用点环境中以数字精度对纳米级物体进行无标记检测

• DOI: 10.1016/j.bios.2023.115197
• 发表时间: 2023
• 期刊: Biosensors and Bioelectronics
• 影响因子: 12.6
• 作者: Liu, Leyang;Tibbs, Joseph;Li, Nantao;Bacon, Amanda;Shepherd, Skye;Lee, Hankeun;Chauhan, Neha;Demirci, Utkan;Wang, Xing;Cunningham, Brian T.
• 通讯作者: Cunningham, Brian T.

Net-Shaped DNA Nanostructures Designed for Rapid/Sensitive Detection and Potential Inhibition of the SARS-CoV-2 Virus

• DOI: 10.1021/jacs.2c04835
• 发表时间: 2022-07-26
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Chauhan, Neha;Xiong, Yanyu;Wang, Xing
• 通讯作者: Wang, Xing