Development and validation of a three-dimensional electro-chemo-mechanical model for the discription of the urinary bladder

用于描述膀胱的三维电化学机械模型的开发和验证

• 批准号: 244579774
• 负责人: Professor Dr. Reinhard Blickhan
• 金额: --
• 依托单位: Lehrstuhl für Bewegungswissenschaft
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/244579774?language=en
• 关键词: Development; validation; three; dimensional; electro

The urinary bladder is a central organ of vertebrates and imposes, based on its extreme deformation (volume changes up to several 100%), special requirements on the entire bladder tissue and especially on the active smooth muscle tissue. Although there are many dysfunctions combined with tissue changes, mechanical analyses are rare. Consequently, the main aim of this project is the development and validation of an electro-chemo-mechanical model based on experimental investigations on the swine urinary bladder.For the development and validation of the model, four steps are scheduled. Thereby, experiments on domestic pigs will be performed, as structure and contraction behaviour are similar to those of humans. Basic requirement for the model generation is the development of the used techniques (I). These are the registration of the three-dimensional deformation as well as of the activation potential propagation at the bladder's surface. Based on an optical measurement technique, developed by the applicants for the recording of skeletal muscles deformation, in this project a procedure will be established to measure the bladder geometry in physiological saline solution. Further, surface electrodes for the measurement of the propagation of the action potential at the bladder's surface will be developed. The first experimental step includes analyses of tissue strips (II). In order to capture local differences of the bladder's structure, preparations will be dissected at defined positions of the bladder wall. Thus, the layer specific architecture as well as its active and passive characteristics can be determined. These data are used for an interim validation and the parameter identification of the electro-chemo-mechanical model. Based on active and passive analyses on the whole bladder (III) its capacity, the pressure-volume dependence, the three-dimensional deformation and the propagation of the action potential at the bladder's surface will be determined. In a final step these additional data of the whole bladder are used to validate the whole model (IV).The model developed in this project can be seen to be a compensational method for animal models and, based on the similarity between human and porcine bladders, for human studies. Thus, it can contribute in future to a reduction of animal experiments and can help to have a more comprehensive understanding of bladder functions. Prospective developments of the model could deal with the prediction of different functional impacts of tissue changes (i.e. cicatrisation of the muscle layer due to interstitial cystitis). Therefore, morphological and mechanical changes of the sickened tissue will be determined experimentally and implemented and converted into the model.

膀胱是脊椎动物的中心器官，由于其极端变形（体积变化高达数百％），对整个膀胱组织，尤其是活跃的平滑肌组织提出了特殊要求。尽管存在许多与组织变化相结合的功能障碍，但机械分析很少见。因此，该项目的主要目的是基于猪膀胱的实验研究开发和验证电化学机械模型。为了模型的开发和验证，安排了四个步骤。因此，将在家猪上进行实验，因为其结构和收缩行为与人类相似。模型生成的基本要求是所使用技术的开发（I）。这些是三维变形以及膀胱表面激活电位传播的记录。基于由申请人开发的用于记录骨骼肌变形的光学测量技术，在该项目中将建立一种程序来测量生理盐水溶液中的膀胱几何形状。此外，还将开发用于测量膀胱表面动作电位传播的表面电极。第一个实验步骤包括组织条的分析 (II)。为了捕捉膀胱结构的局部差异，将在膀胱壁的指定位置解剖制剂。因此，可以确定层特定架构及其主动和被动特性。这些数据用于电化学机械模型的临时验证和参数识别。基于对整个膀胱 (III) 的主动和被动分析，将确定其容量、压力-体积依赖性、三维变形和动作电位在膀胱表面的传播。在最后一步中，整个膀胱的这些附加数据用于验证整个模型（IV）。该项目中开发的模型可以被视为动物模型的补偿方法，并且基于人类和猪膀胱之间的相似性，用于人类研究。因此，它可以在未来有助于减少动物实验，并有助于更全面地了解膀胱功能。该模型的未来发展可以预测组织变化的不同功能影响（即间质性膀胱炎引起的肌肉层疤痕）。因此，病变组织的形态和力学变化将通过实验确定并实施并转化为模型。