SitS Socializing Soil: Enhancing Community CoOperation with Iterative Sensor Research (S3-ECO-wISeR)

SitS 社交化土壤：通过迭代传感器研究加强社区合作 (S3-ECO-wISeR)

• 批准号: 2226714
• 负责人: Shayla Sawyer
• 金额: $ 119.18万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2226714&HistoricalAwards=false
• 关键词: SitS; Socializing; Soil; Enhancing; Community

Community-engaged soil sensing is essential for confronting the challenge of toxic pollution—specifically arsenic and per- and poly-fluoroalkyl substances (PFAS)—in human environments. This project uses bacteria as sensors of soil contaminants, ultimately leading to the production of paper-based “test strips” that generate a quantitative reading of contaminant concentrations in soil. Such biosensors can support basic science as well as community-engaged investigations of exposure pathways. The project uses a “responsible research and innovation” (RRI) framework, which means including stakeholders—particularly citizen scientists—in the research process to enhance capacity to anticipate consequences of different sensor designs, reflect on the researchers’ assumptions, and respond to stakeholder needs and values.This work aims to create a biohybrid device that can exploit and tune the strengths of both electronic and biological sensing with the invention of a dynamic bioelectronic interface. By separating the actuation and sensing stages with different bacteria, a transfer function between signal gain and system stability can be engineered and customized toward community sensing needs. Development of the device involves pursuit of four interdependent aims: 1) engaging stakeholders through citizen science and an advisory board in order to inform research priorities and approaches, 2) using two different bacteria that function as the sensing front end (E.coli) and electroactive backend (S. oneidensis) to produce arsenic detection at detection levels comparable to current standards, 3) experimentation with electroactive bacteria species (P. aeroginosa) to advance toward a PFAS biosensor, and 4) iterative development of a paper-based sensor design that is suitable for community-based environmental monitoring.This award was made through the "Signals in the Soil (SitS)" solicitation, a collaborative partnership between the National Science Foundation and the United States Department of Agriculture National Institute of Food and Agriculture (USDA NIFA).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

社区参与的土壤传感对于应对人类环境中的有毒污染-特别是砷和全氟烷基和多氟烷基物质（PFAS）-的挑战至关重要。该项目使用细菌作为土壤污染物的传感器，最终导致生产纸基“测试条”，产生土壤中污染物浓度的定量阅读。这种生物传感器可以支持基础科学以及社区参与的接触途径调查。该项目使用“负责任的研究与创新”（RRI）框架，这意味着将利益相关者--特别是公民科学家--纳入研究过程，以增强预测不同传感器设计后果的能力，反思研究人员的假设，这项工作的目的是创造一种生物混合装置，可以利用和调整电子和生物传感的优势，动态生物电子接口的发明通过分离具有不同细菌的致动和感测阶段，信号增益和系统稳定性之间的传递函数可以针对群体感测需求进行设计和定制。该设备的开发涉及追求四个相互依存的目标：1）通过公民科学和咨询委员会吸引利益相关者，以告知研究优先事项和方法，2）使用两种不同的细菌作为传感前端（大肠杆菌）和电活性后端（S。oneidensis）以产生与当前标准相当的检测水平的砷检测，3）用电活性细菌物种进行实验（P. aeroginosa）向PFAS生物传感器发展，以及4）迭代开发适合社区环境监测的纸基传感器设计。该奖项是通过“土壤中的信号（SitS）”征集获得的，美国国家科学基金会与美国农业部国家食品和农业研究所（USDA NIFA）之间的合作伙伴关系。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。