SitS Socializing Soil: Enhancing Community CoOperation with Iterative Sensor Research (S3-ECO-wISeR)

SitS 社交化土壤：通过迭代传感器研究加强社区合作 (S3-ECO-wISeR)

• 批准号: 2226714
• 负责人: Shayla Sawyer
• 金额: $ 119.18万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2226714&HistoricalAwards=false
• 关键词: SitS; Socializing; Soil; Enhancing; Community

Community-engaged soil sensing is essential for confronting the challenge of toxic pollution—specifically arsenic and per- and poly-fluoroalkyl substances (PFAS)—in human environments. This project uses bacteria as sensors of soil contaminants, ultimately leading to the production of paper-based “test strips” that generate a quantitative reading of contaminant concentrations in soil. Such biosensors can support basic science as well as community-engaged investigations of exposure pathways. The project uses a “responsible research and innovation” (RRI) framework, which means including stakeholders—particularly citizen scientists—in the research process to enhance capacity to anticipate consequences of different sensor designs, reflect on the researchers’ assumptions, and respond to stakeholder needs and values.This work aims to create a biohybrid device that can exploit and tune the strengths of both electronic and biological sensing with the invention of a dynamic bioelectronic interface. By separating the actuation and sensing stages with different bacteria, a transfer function between signal gain and system stability can be engineered and customized toward community sensing needs. Development of the device involves pursuit of four interdependent aims: 1) engaging stakeholders through citizen science and an advisory board in order to inform research priorities and approaches, 2) using two different bacteria that function as the sensing front end (E.coli) and electroactive backend (S. oneidensis) to produce arsenic detection at detection levels comparable to current standards, 3) experimentation with electroactive bacteria species (P. aeroginosa) to advance toward a PFAS biosensor, and 4) iterative development of a paper-based sensor design that is suitable for community-based environmental monitoring.This award was made through the "Signals in the Soil (SitS)" solicitation, a collaborative partnership between the National Science Foundation and the United States Department of Agriculture National Institute of Food and Agriculture (USDA NIFA).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在人类环境中，社区参与的土壤敏感性对于面对有毒污染的挑战（特定于砷，均和多氟烷基物质（PFA）至关重要。该项目使用细菌作为土壤污染物的传感器，最终导致产生基于纸张的“测试条”，从而产生对土壤中污染物浓度的定量读数。这样的生物传感器可以支持基础科学以及社区参与的曝光途径投资。该项目使用“负责任的研究与创新”（RRI）框架，其意思是包括利益相关者（尤其是公民科学家）在研究过程中，以增强能力来预测不同传感器设计的后果，反思研究人员的假设，并响应利益相关者的需求和价值。这项工作旨在创建一种生物杂交设备，该设备可以利用和调整电子和生物感测的强度，以动态生物电子界面的意图。通过将动作和传感阶段与不同的细菌分开，可以对信号增益和系统稳定性之间的传递函数进行设计并根据社区敏感性需求进行定制。该设备的开发涉及追求四个相互依存的目的：1）通过公民科学和一个顾问委员会吸引利益相关者，以告知研究优先级和方法，2）使用两个不同的细菌作为传感前端（E.COLI）（E.COLI）（E.COLI）（E.COLI）（E.COLI）（E.COLI）和ELEPERTROACTIVE BACEDEND（S. s. ONEIDENSIS）在检测级别上产生ARSENIC COMPOTIRIA级别的均等级别，3） Aeroginosa）要迈向PFA生物传感器，4）迭代开发基于纸张的传感器设计，适用于社区的环境监测。该奖项是通过“土壤中的信号（SITS）”征求的，这是国家科学基金会与美国国家科学局之间的合作合作伙伴关系。任务，并通过评估使用基金会的知识分子和更广泛的影响审查标准，被认为是宝贵的支持。