Ultrasensitive marker-free biomolecular THz-detection for tumor related analytics: PCR-free sensitivity, biomolecular extension and biological relevance

用于肿瘤相关分析的超灵敏无标记生物分子太赫兹检测：无 PCR 灵敏度、生物分子延伸和生物相关性

• 批准号: 272383922
• 负责人: Professorin Dr. Anja-Katrin Bosserhoff
• 金额: --
• 依托单位: Lehrstuhl für Höchstfrequenztechnik und Quantenelektronik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/272383922?language=en
• 关键词: Ultrasensitive; marker; free; biomolecular; THz

Genomics and proteomics hold promise to revolutionize future health care, targeting profound novel visions like predictive medicine, preventive medicine and personalized medicine. The enormous advance in these fields is closely coupled to the development of powerful, reliable, and efficient methods to detect, identify and analyse biomolecules and their complex interaction networks. In the last decades techniques have continuously improved, providing a plethora of information for biomolecular function. However, as we are at the stage of moving from “observing” dependencies, towards “understanding” mechanisms in their full interaction network, it is becoming increasingly apparent that new analytic tools with enhanced capabilities, providing information at various biomolecular interaction levels still need to be developed.THz technologies have demonstrated a huge potential to investigate and detect biomolecular systems, as macromolecular modes and biomolecular interactions are resonant in this frequency range, paving the path to label-free bioanalytic sensing. The goal of this project is the development of competitive principles for the label–free biomolecular sensing with electromagnetic radiation (EM) in the THz frequency range. The project follows upon the successful demonstration of PCR-free DNA sensing capabilities in frequency selective surfaces. We propose to study its potential for ultrasensitive marker-free biomolecular THz-detection for tumor related analytics. After showing world-record PCR-free sensitivities in the first project phase, the activities now concentrate on: extending the applicability to other biomolecular systems and on the demonstration of the fundamental biological relevance of the THz analysis. Reproducibility in protein sensing and sensor functionalization will be addressed by implementing a new activity related to microfluidic integration in this second project phase.In order to focus this research activity and validate the application relevance of these developments, all analysis will concentrate on tumor-related biomolecular sensing applications. Due to emerging new therapies, analyses of biomarker become even more important today. Further, complete understanding of the complex processes in tumor development and progression is tremendously important. In this project, it is therefore planned to concentrate on a detailed comparison of THz biochip results with well-known validated previous results and with state-of-the-art biomolecular investigation methods.

基因组学和蛋白质组学有望彻底改变未来的医疗保健，目标是预测医学，预防医学和个性化医学等深刻的新愿景。这些领域的巨大进步与强大、可靠和有效的方法的发展密切相关，这些方法可以检测、识别和分析生物分子及其复杂的相互作用网络。在过去的几十年里，技术不断改进，为生物分子功能提供了大量的信息。然而，由于我们正处于从“观察”依赖性转向“理解”其完整相互作用网络中的机制的阶段，因此越来越明显的是，仍然需要开发具有增强功能的新分析工具，以提供各种生物分子相互作用水平的信息。太赫兹技术已经证明了研究和检测生物分子系统的巨大潜力，因为大分子模式和生物分子相互作用在该频率范围内共振，从而为无标记生物分析传感铺平了道路。该项目的目标是在太赫兹频率范围内的电磁辐射（EM）的无标记生物分子传感的竞争原则的发展。该项目是在成功展示频率选择表面中的无PCR DNA传感能力之后进行的。我们建议研究其用于肿瘤相关分析的超灵敏无标记生物分子THz检测的潜力。在第一个项目阶段展示了世界纪录的无PCR灵敏度之后，现在的活动集中在：将适用性扩展到其他生物分子系统，并展示THz分析的基本生物相关性。在第二个项目阶段，将通过实施与微流体集成相关的新活动来解决蛋白质传感和传感器功能化的复制问题。为了集中研究活动并验证这些发展的应用相关性，所有分析都将集中在肿瘤相关的生物分子传感应用上。由于新疗法的出现，生物标志物的分析在今天变得更加重要。此外，全面了解肿瘤发展和进展的复杂过程是非常重要的。因此，在该项目中，计划将THz生物芯片结果与已知的验证结果以及最先进的生物分子研究方法进行详细比较。