Characterization of graphene immune-impacts through omics approaches and genotoxic analysis

通过组学方法和基因毒性分析表征石墨烯免疫影响

• 批准号: 279055639
• 负责人: Professor Dr. Björn Schumacher
• 金额: --
• 依托单位: Institut de Biologie Moléculaire et Cellulaire (IBMC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279055639?language=en
• 关键词: Characterization; graphene; immune; impacts; through

Graphene is the most promising, versatile, sustainable key enabling nanotechnology which may bring solutions in the near future to many areas spanning materials science and medicine.However, the potential interactions of graphene with biological systems deriving from its physico-chemical properties and reactivity are raising public concern on risks for human health and environment. Independently from any specific purpose, a critical step for future translational applications is represented by the assessment of graphene impact on the immune system. Following any type of exposures, nanomaterials immediately contact the organism immune cells. Hence the investigation of the immune cell reactions is a milestone for the safe exploitation of graphene for therapeutic and diagnostic applications.The overall objective of G-IMMUNOMICS is to complement Flagship research on graphene safety with immunogenomic and proteomic data, not covered by the Core Project. G-IMMUNOMICS will provide new insights on the immune impact of several types of graphene to lay the basis for a safe use of graphene and the graphene technology future transfer in medicine. The specific objectives are:1. Produce highly stable and dispersible pristine and functionalized graphene family materials (GFMs) with different lateral size and appropriate functionalizations2. Characterize by high throughput functional immunogenomics and proteomics approaches and genotoxic assays the immune cell response induced by GFMs on different cell lines and primary cells3. Evaluate the immune cell response induced by GFMs on four different species: human, mouse, pig and worm.G-IMMUNOMICS integrates with the FLAGSHIP at 3 levels- Scientific. collaborations are ongoing with many partners of the Flagship; in particular the project will synergize with the WP2 Flagship objectives. The project will: 1) extend the range of immune cell types investigated with a wide variety of populations and species; 2) implement large scale OMICS research approaches assays (genomics and proteomics); 3) take part in assessment of the environmental impact of new materials by analysing the genotoxic effect on different organisms.- Operational. The work plan builds on deep connections with the FLAGSHIP, due to: 1) exploitation of synthesis protocol Flagship developed by WP1 and WP10; 2) testing of GFMs provided by Flagship WP2 and WP10 partners; 3) usage of human ex vivo and mouse in vivo samples for genomic, proteomic and genotoxic analysis provided by Flagship WP2.- Dissemination. The results gained during the project will be shared with Flagship partners during WP meetings and general assemblies in order to boost the transfer of knowledge achieved on graphene.Outcomes: G-IMMUNOMICS research may outline potential pathogenic biomarkers (genes, miRNAs and proteins) associated with GFMs exposure, assess their overall safety profile and identify highly biocompatible GFMs suitable for future medical exploitation.

石墨烯是最有前途的、多用途的、可持续的关键使能纳米技术，在不久的将来可能为材料科学和医学的许多领域带来解决方案。然而，石墨烯的物理化学性质和反应性与生物系统的潜在相互作用引起了公众对人类健康和环境风险的关注。独立于任何特定目的，未来转化应用的关键步骤是评估石墨烯对免疫系统的影响。在任何类型的暴露之后，纳米材料立即接触生物体免疫细胞。因此，对免疫细胞反应的研究是石墨烯在治疗和诊断应用中安全开发的一个里程碑。G-IMMUNOMICS的总体目标是用免疫基因组学和蛋白质组学数据补充石墨烯安全性的旗舰研究，这些数据不在核心项目中。G-IMMUNOMICS将提供关于几种类型石墨烯的免疫影响的新见解，为石墨烯的安全使用和石墨烯技术未来在医学中的转移奠定基础。具体目标是：1. 生产具有不同横向尺寸和适当功能化的高度稳定和可分散的原始和功能化石墨烯家族材料（GFM）2。 通过高通量功能性免疫基因组学和蛋白质组学方法以及基因毒性测定来表征不同细胞系和原代细胞上GFM诱导的免疫细胞应答3。 评估GFM在四种不同物种（人、小鼠、猪和蠕虫）上诱导的免疫细胞反应。G-IMMUNOMICS在3个级别上与旗舰集成- Scientific。目前正在与旗舰项目的许多合作伙伴开展合作;特别是该项目将与WP 2旗舰项目的目标协同增效。该项目将：1）扩大免疫细胞类型的范围，研究各种人群和物种; 2）实施大规模OMICS研究方法测定（基因组学和蛋白质组学）; 3）通过分析对不同生物体的遗传毒性效应，参与评估新材料的环境影响。运作中。该工作计划建立在与旗舰公司的深层联系之上，原因是：1）利用WP 1和WP10开发的合成方案; 2）测试旗舰公司WP 2和WP10合作伙伴提供的GFM; 3）使用旗舰公司WP 2提供的人类离体和小鼠体内样品进行基因组，蛋白质组学和遗传毒性分析。传播。项目期间获得的成果将在WP会议和大会期间与旗舰合作伙伴分享，以促进石墨烯知识的转移。成果：G-IMMUNOMICS研究可能概述与GFM暴露相关的潜在致病生物标志物（基因，miRNA和蛋白质），评估其整体安全性，并确定适合未来医疗开发的高度生物相容性GFM。

项目成果

Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans.

• DOI: 10.1002/smll.201902699
• 发表时间: 2019-10
• 期刊: Small
• 影响因子: 13.3
• 作者: Corvin Rive;G. Reina;Prerana Wagle;E. Treossi;V. Palermo;A. Bianco;Lucia Gemma-Delogu;Matthias Rieckher;B. Schumacher