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Testing recurrent ecological speciation processes in microendemic Madagascan reptiles and amphibians of the isolated Amber Mountain

测试孤立琥珀山微流行马达加斯加爬行动物和两栖动物的循环生态物种形成过程

基本信息

批准号：
322418141
负责人：
Professor Dr. Miguel Vences
金额：
--
依托单位：
Zoologisches Institut
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/322418141?language=en
关键词：
Testing recurrent ecological speciation processes

项目摘要

The processes by which new species arise have long been an active field of debate Current research on speciation focuses the mechanisms driving the divergence. Ecological Speciation (ES) occurs if natural selection strongly favours different ecotypes, and reproductive isolation evolves as a consequence of adaptive divergence due to differing ecology. However, suitable settings to study ES are rare. Our long-term research program on the evolution of the vertebrate fauna of Madagascar has yielded preliminary indicated that the isolated Amber Mountain Massif in northern Madagascar harbours distinct locally endemic sister clades of various amphibians and reptiles, and some of these appear to be restricted to distinct elevational ranges. This suggests that these lineages might have originated in the Massif by ES, and points to Amber Mountain as a suitable model system for studying diversification across ecological (elevational) gradients. The research project proposed here will combine cutting-edge methods in metabarcoding and population genetics, to explore ES processes in the microendemic herpetofauna of this massif. It applies a strict hypothesis-testing framework to assess whether an ecological gradient is the predominant driver of past and ongoing ES. Our approach will use metabarcoding data for the entire herpetofaunal community of Amber Mountain, and population genetic in-depth study of three target taxa (likely including one or several of the genera Brookesia, Stumpffia, Phelsuma, or Uroplatus). Dense sampling will be carried out along pre-defined transects that will be characterized ecologically, and furthermore, rapid surveys will be done using a grid approach in order to characterize the distribution and phylogeography of the entire herpetofauna in the massif. The large number of samples collected during fieldwork will be analyzed using high-throughput amplicon metabarcoding of pooled samples with Illumina sequencing. For the target taxa, microsatellites and RADseq-derived SNPs will be used to characterize small-scale population differentiation. If ES triggered the diversification of amphibians and reptiles of Amber Mountain, then we predict that (i) endemic sister lineages that differentiated on Amber Mountain will be distributed at distinct elevational ranges, (ii) their contact zones coincide with ecotones and with turnover of the entire herpetofaunal community, (iii) target lineages distributed across these ecotones will show reduction of gene flow related to the ecological gradient rather than to distance or physical barriers. Given the scarcity of research that tests ES in Madagascar, the endeavour to establish the Amber Mountain as study region for such research would be ground-breaking, and stimulate process-based speciation research in this unique biota.

新物种产生的过程长期以来一直是一个活跃的争论领域，目前关于物种形成的研究集中在驱动物种分化的机制上。如果自然选择强烈地偏爱不同的生态型，就会发生生态物种形成，而生殖隔离是由于不同的生态而导致的适应性分化的结果。然而，适合研究ES的环境并不多见。我们对马达加斯加脊椎动物区系演化的长期研究计划初步表明，马达加斯加北部与世隔绝的琥珀山丘上有不同的当地特有的两栖动物和爬行动物姐妹支系，其中一些似乎限于不同的海拔范围。这表明这些谱系可能起源于Es的Massif，并指出琥珀山是研究生态(海拔)梯度多样性的合适模式系统。本文提出的研究项目将结合后生生物编码和种群遗传学的前沿方法，探索该地块微型地方性爬行动物的ES过程。它应用了一个严格的假设检验框架来评估生态梯度是否是过去和正在进行的生态系统的主要驱动因素。我们的方法将使用整个琥珀山两栖动物群落的后生生物编码数据，以及对三个目标分类群(可能包括Brookesia属、Stumpffia属、PhelSuma属或Uroplatus属的一个或几个)的种群遗传深入研究。将沿着预定的样带进行密集采样，这些样带将具有生态特征，此外，将使用网格方法进行快速调查，以确定整个地块中爬行动物的分布和系统地理特征。在实地工作中收集的大量样本将使用高通量扩增子变位编码与Illumina测序相结合的方式进行分析。对于目标类群，将使用微卫星和RADseq衍生的SNPs来描述小规模种群分化的特征。如果ES触发了琥珀山两栖和爬行动物的多样化，那么我们预测：(I)在琥珀山上分化的特有姐妹系将分布在不同的海拔范围，(Ii)它们的接触带与交错带重合，并伴随着整个两栖动物群落的更替，(Iii)分布在这些交错带的目标谱系将显示与生态梯度有关的基因流减少，而不是距离或物理障碍。鉴于马达加斯加缺乏测试ES的研究，将琥珀山建立为这类研究的研究区域的努力将是开创性的，并促进在这一独特生物群中进行基于过程的物种形成研究。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sympatric lineages in the Mantidactylus ambreensis complex of Malagasy frogs originated allopatrically rather than by in-situ speciation.

DOI：
10.1016/j.ympev.2019.106700
发表时间：
2019-12
期刊：
Molecular phylogenetics and evolution
影响因子：
4.1
作者：
Safidy M Rasolonjatovo;M. Scherz;C. Hutter;F. Glaw;Andolalao Rakotoarison;Jary H. Razafindraibe;S. Goodman-S
通讯作者：
Safidy M Rasolonjatovo;M. Scherz;C. Hutter;F. Glaw;Andolalao Rakotoarison;Jary H. Razafindraibe;S. Goodman-S

Computational molecular species delimitation and taxonomic revision of the gecko genus Ebenavia Boettger, 1878