Biochemische Charakterisierung der Ribonukleoprotein-vermittelten Mobiliät von non-LTR-Retrotransposons

核糖核蛋白介导的非 LTR 逆转录转座子移动性的生化特征

• 批准号: 36166581
• 负责人: Professorin Dr. Nora Zingler
• 金额: --
• 依托单位: Fachbereich Biologie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/36166581?language=en
• 关键词: Biochemische; Charakterisierung; der; Ribonukleoprotein; vermittelten

Non-LTR retrotransposons are mobile genetic elements found in almost every eukarotic organism. Their unparalleled impact on genome plasticity and their elaborate RNA-mediated replication mechanism (target primed reverse transcription, TPRT) make them important subjects of study both from an evolutionary and biochemical point of view. In this study, I will set up a new model system to biochemically characterize the RNA/protein machinery that performs the retrotransposition process. By probing the structure of this ribonucleoprotein complex with chemical modification and crosslinking experiments, I will map the RNA/protein interface. Mechanistic studies will involve in-depth kinetic analysis of the reverse transcription process using a variety of different substrates. The results of this study will shed light on how large portions of the genome are generated and enable us to better manipulate retrotransposons for biotechnological and medical purposes. Besides, comparison with related systems like telomerases and group II introns will provide us with new insights into how RNA and proteins cooperate and how they change their functions during evolution.

非ltr反转录转座子是几乎在所有真核生物中发现的可移动遗传元件。它们对基因组可塑性的影响无与伦比，其复杂的rna介导的复制机制（靶引反转录，TPRT）使它们成为从进化和生化角度研究的重要课题。在这项研究中，我将建立一个新的模型系统来生化表征执行逆转录过程的RNA/蛋白质机制。通过化学修饰和交联实验探测这种核糖核蛋白复合物的结构，我将绘制RNA/蛋白质界面。机制研究将涉及使用各种不同底物对逆转录过程进行深入的动力学分析。这项研究的结果将阐明基因组的大部分是如何产生的，并使我们能够更好地为生物技术和医学目的操纵反转录转座子。此外，与端粒酶和II组内含子等相关系统的比较将为我们提供RNA和蛋白质如何合作以及它们在进化过程中如何改变其功能的新见解。