Fractionation of nanoparticles by preparative gel electrophoresis

通过制备型凝胶电泳分离纳米颗粒

• 批准号: 382141490
• 负责人: Professor Dr. Georg Garnweitner
• 金额: --
• 依托单位: Institut für Partikeltechnik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/382141490?language=en
• 关键词: Fractionation; nanoparticles; preparative; gel; electrophoresis

Gel electrophoresis is an established method for the highly selective analytical and preparative fractionation of macromolecules. The separation of nanoparticles by gel electrophoresis has been studied in some works, however it has hardly been studied for application-relevant materials such as metal oxides, and general process knowledge enabling the fast and efficient purification is missing. The aim of this project is to establish gel electrophoresis fractionation of metal oxide nanoparticles and to perform extensive process investigations. By using model materials with defined properties, such as spherical SiO2 nanoparticles with variable size and ZnO nanostructures with different, controllable morphologies and defined surface chemistry, a broad understanding of the suitability of this method for the fractionation of nanoparticles is developed. Furthermore, a specially designed 2D gel electrophoresis device is used on the one hand for the preparative (semi-) continuous separation and, on the other hand, for a multidimensional separation according to e.g. size and surface charge. This is complemented by mathematical modelling, first based on empirical results, and is extended in the second project phase by a simulation taking into account the gel structure.

凝胶电泳是一种成熟的高选择性分析和制备大分子分离的方法。凝胶电泳分离纳米颗粒的研究已经有一些成果，但对金属氧化物等应用相关材料的研究很少，而且缺乏能够实现快速高效纯化的一般工艺知识。本项目的目的是建立金属氧化物纳米颗粒的凝胶电泳分馏法，并进行广泛的工艺研究。通过使用具有特定性质的模型材料，如具有可变尺寸的球形SiO2纳米颗粒和具有不同、可控形貌和确定表面化学性质的ZnO纳米结构，对该方法在纳米颗粒分选中的适用性有了广泛的了解。此外，专门设计的二维凝胶电泳装置一方面用于制备（半）连续分离，另一方面用于根据尺寸和表面电荷等进行多维分离。这是通过数学建模来补充的，首先基于经验结果，并在第二个项目阶段通过考虑凝胶结构的模拟来扩展。