Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles

在这项研究中，我们应用声动力学治疗癌细胞的基础上交付的二氧化钛（TiO 2）纳米粒子（NPs）与抗生物素蛋白修饰，优先区分癌细胞从健康细胞。随后，羟基自由基产生的TiO 2纳米粒子活化后，通过外部超声辐射（TiO 2/US处理）。尽管30%的正常乳腺细胞（人乳腺上皮细胞）表现出抗生物素蛋白修饰的TiO 2纳米颗粒的摄取，但超过80%的乳腺癌细胞（MCF-7）表现出抗生物素蛋白-TiO 2纳米颗粒的摄取。接下来，对掺入TiO 2 NP的细胞施加1 MHz超声（0.1 W/cm 2，30 s）后，检查TiO 2/US处理对MCF-7细胞生长的影响长达96小时。处理后24 h细胞未见明显损伤，96 h活细胞浓度降至对照组的68%。

In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm2, 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24 h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.