scyllo-inositol production using inositol catabolic pathway in Paracoccus sp. 43P.
scyllo-inositol production using inositol catabolic pathway in Paracoccus sp. 43P.
批准号:
24658069
负责人:
NAKAMURA Akira
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
中文摘要
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英文摘要
In this study, we aimed to develop a scyllo-inositol (SI) production system from myo-inositol (MI), using MI dehydrogenase (IolG) and scyllo-inosose reductase (LgdA) of Paracoccus sp. 43P. In vitro reaction with both the enzymes produced 17.8 mM SI from 50 mM MI after 10 h reaction, showing that these enzymes can be used for SI production, with relatively high conversion rate (35%).The iolE deletion mutant of strain 43P, which blocks downstream catabolism of scyllo-inosose, was constructed and used for bioconversion of MI to SI. This strain achieved about 20% conversion to SI after 96 h cultivation in a minimal medium with galactose as a carbon source.
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