scyllo-inositol production using inositol catabolic pathway in Paracoccus sp. 43P.

使用副球菌中的肌醇分解代谢途径生产鲨肌醇。

• 批准号: 24658069
• 负责人: NAKAMURA Akira
• 金额: $ 2.58万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658069/
• 关键词: scyllo-inositol; myo-inositol; Paracoccus sp. 43P; bioconversion; Paracoccus; inositol dehydrogenase

In this study, we aimed to develop a scyllo-inositol (SI) production system from myo-inositol (MI), using MI dehydrogenase (IolG) and scyllo-inosose reductase (LgdA) of Paracoccus sp. 43P. In vitro reaction with both the enzymes produced 17.8 mM SI from 50 mM MI after 10 h reaction, showing that these enzymes can be used for SI production, with relatively high conversion rate (35%).The iolE deletion mutant of strain 43P, which blocks downstream catabolism of scyllo-inosose, was constructed and used for bioconversion of MI to SI. This strain achieved about 20% conversion to SI after 96 h cultivation in a minimal medium with galactose as a carbon source.

本研究以副球菌43 P的肌醇脱氢酶（IolG）和鲨肌醇还原酶（LgdA）为酶源，建立了鲨肌醇生产系统。体外实验表明，两种酶均能将50 mM的MI转化为17.8 mM的SI，转化率较高（35%）。该菌株在以半乳糖作为碳源的基本培养基中培养96小时后，实现了约20%向SI的转化。