Elusidation of microstructure of biofilm attached on the surface of agricultural produce

农产品表面生物膜微观结构的解析

• 批准号: 24658212
• 负责人: UCHINO Toshitaka
• 金额: $ 2.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658212/
• 关键词: バイオフィルム; 共焦点レーザー顕微鏡; 細胞外多糖類; 乳製品副産物; インデンテーション法; 微細構造

An effect of dairy byproducts on adherence of dairy related bacteria to a stainless steel coupon surface conditioned by the byproducts was evaluated. The layer formed by skim milk buttermilk butter serum was thinnest, medium and thickest, respectively. Three dimensional images of biofilm were obtained by using a confocal laser scanning microscopy. In the result the conditioning by the byproducts controlled the bacterial adherence.A hardness of biofilm was determined by indentation method. Twenty micro m of loading and 10 micro m of unloading by a universal material testing machine were repeated until a plunger head touching a plastic coupon. Thickness of biofilm was calculated from a length between contact points of the plunger head with the coupon and biofilm. The biofilm strength increased with decrease of biofilm thickness.

评价了乳制品副产物对乳制品相关细菌粘附到由副产物调节的不锈钢试样表面的影响。脱脂乳、酪乳、奶油清形成的层最薄、中等、最厚。利用共聚焦激光扫描显微镜获得生物膜的三维图像。结果表明，副产物的调理作用控制了细菌的粘附。通过通用材料试验机重复20微米的加载和10微米的卸载，直到柱塞头接触塑料试样。从柱塞头与试样和生物膜的接触点之间的长度计算生物膜的厚度。生物膜强度随生物膜厚度的减小而增大。

项目成果

Surface conditioning of stainless steel coupons with skim milk, buttermilk, and butter serum solutions and its effect on bacterial adherence

脱脂奶、酪乳和黄油血清溶液对不锈钢试样的表面调节及其对细菌粘附的影响

• DOI: 10.1016/j.foodcont.2014.01.040
• 发表时间: 2014
• 期刊: Food Control
• 影响因子: 6
• 作者: M. D. Nguyen;L.D. Manh;D. Hamanaka;H. V. Duong;F. Tanaka;T. Uchino
• 通讯作者: T. Uchino

バイオフィルムを形成したPseudomonas putidaへの洗浄効果

对已形成生物膜的恶臭假单胞菌的净化效果

• 发表时间: 2012
• 作者: Nguyen Manh Dat;Le Duc Manha;Daisuke Hamanaka;Duong Van Hung;Fumihiko Tanaka;Toshitaka Uchino;江口広大,内野敏剛,田中史彦,濱中大介,森松和也
• 通讯作者: 江口広大,内野敏剛,田中史彦,濱中大介,森松和也

インデンテーション法によるバイオフィルムの力学特性評価の可能性

使用压痕法评估生物膜机械性能的可能性

• 发表时间: 2013
• 期刊: 農業食料工学会九州支部誌
• 作者: M. D. Nguyen;L.D. Manh;D. Hamanaka;H. V. Duong;F. Tanaka;T. Uchino;江口広大,堀海,森本愛子,濱中大介,金山素平,田中史彦,内野敏剛
• 通讯作者: 江口広大,堀海,森本愛子,濱中大介,金山素平,田中史彦,内野敏剛

Influence of some dairy by-products on the adherence of Lactococcus lactis

一些乳制品副产品对乳酸乳球菌粘附的影响

• 发表时间: 2013
• 作者: M. D. Nguyen;L.D. Manh;D. Hamanaka;H. V. Duong;F. Tanaka;T. Uchino
• 通讯作者: T. Uchino