A challenge to construct human mini liver using temperature sensitive immortalized human liver cells

使用温度敏感的永生化人肝细胞构建人迷你肝脏的挑战

• 批准号: 24659067
• 负责人: KAN Chiba
• 金额: $ 2.5万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659067/
• 关键词: 評価系; 不死化細胞; 肝臓; 薬物代謝; 予測; 薬物動態・代謝スクリーニング系; ヒトの薬物動態・代謝予測系

In the present study, we have successfully developed novel human immortalized liver sinusoidal endothelial cells (hiLSEC) using a two immortalization gene introduction method. The cells show high proliferation ability as well as cell differentiation characteristics. In addition, we have obtained a new easy and effective culture method for use in hepatic-cell differentiation. Therefore, a new in vitro human liver model can be developed using hiLSEC in conjunction with hepatic cells cultured using the new method, and it can be predicted that the novel in vitro human liver model will represent a significant and useful advancement in the science of in vitro liver modeling. We hope that this new model will facilitate studies of pharmacokinetics/toxicity research as well as those aimed at clarifying hepatic differentiation mechanisms and exploring new drug targets for liver diseases, such as liver fibrosis.

在本研究中，我们采用双永生化基因导入的方法成功地培育了新型人肝窦内皮细胞（hiLSEC）。细胞表现出较高的增殖能力和细胞分化特征。此外，我们还获得了一种简便有效的肝细胞分化培养新方法。因此，利用hiLSEC结合新方法培养的肝细胞，可以建立一种新的体外人肝模型，可以预见，这种新的体外人肝模型将是体外肝脏建模科学的一个重要而有用的进展。我们希望这一新模型能够促进药代动力学/毒性研究，以及旨在阐明肝脏分化机制和探索肝脏疾病（如肝纤维化）的新药物靶点的研究。