Role of INTS6 in the adipose differentiation

我们发现，在3T3-L1细胞中，整合子复合体的亚基fints6和IntS11的表达水平在细胞达到ed汇合并分化为脂肪细胞时升高，而在分化完成后则降至基础水平。利用sirna抑制3t3 - l1前脂肪细胞中IntS6或IntS11的表达可显著抑制成熟脂肪细胞的分化。这些发现表明，在脂肪分化过程中，整合子复合体亚基的表达增加是不可或缺的。虽然需要进一步的研究来阐明其潜在的机制，但U1， U2小核rna的加工可能参与了细胞分化的步骤。

We demonstrated that expression levels ofIntS6 and IntS11, subunits ofthe Integrator complex, were increased in 3T3-L1 cells in the period when the cells reach edconfluenceand differentiated into adipocytes, while being reduced tobasal levels after the completion ofdifferentiation. Suppre ssion of IntS6 or IntS11 expression using siRNAs in3T3-L1 pre-adipocytes markedly inhibited differentiation into mature adipocytes. These findings demonstrate that increased expression of Integrator complex subunits is an indispensable event in adipose differentiation. Although further study is necessary to elucidate the underlying mechanism, the processing of U1, U2 small nuclear RNAs may be involved in cell differentiation steps.