Study of the expression of keratan sulfate in gynecological malignancies

硫酸角质素在妇科恶性肿瘤中表达的研究

• 批准号: 24659725
• 负责人: SHIOZAWA Tanri
• 金额: $ 2万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659725/
• 关键词: ケラタン硫酸; 婦人科悪性腫瘍; 子宮内膜症; 鑑別診断; 女性生殖管; 異所性子宮内膜; 子宮内膜癌; 卵巣癌; 診断マーカー

The immunohistochemical staining using monoclonal antibody for keratan sulfate (KS) revealed that the expression of KS was observed in 96.2% (25/26 cases) of non-neoplastic epithelia in female genital tract including fallopian tube, ovarian inclusion cyst, endocervical gland, eutopic and ectopic endometrium. In contrast, the expression of KS was negligible in digestive organ (1/47 cases, 2.3%) and urinary tract (0/6 cases, 0%). In addition, the expression of KS was observed in 90.6% (29/32 cases) of carcinomas in female genital tract (endometrial, tubal and ovarian carcinoma), whereas observed in 0% (0/33 cases) of other carcinomas in abdominal cavity (digestive tract, liver, kidney and urinary tract). These findings suggest that the immunostaining for KS is useful to discriminate epithelia and carcinomas of female genital tract in abdominal cavity. On the other hand, KS-positive cases were present in breast, lung, thyroid, pancreatic cancer and malignant mesothelioma.

硫酸角质素（keratan sulfate，KS）单克隆抗体免疫组化染色显示，KS在女性生殖道非肿瘤上皮中的表达率为96.2%（25/26），包括输卵管、卵巢包涵体囊肿、宫颈管腺体、在位和异位子宫内膜。KS在消化道（1/47例，2.3%）和泌尿道（0/6例，0%）中的表达可忽略不计。KS在女性生殖道癌（子宫内膜癌、输卵管癌和卵巢癌）中的表达率为90.6%（29/32），而在其他腹腔癌（消化道癌、肝、肾和泌尿道癌）中的表达率为0%（0/33）。提示KS免疫组化染色对鉴别腹腔内女性生殖道上皮和癌有一定的意义。另一方面，KS阳性病例存在于乳腺癌、肺癌、甲状腺癌、胰腺癌和恶性间皮瘤中。

项目成果

Sirtuin1(SIRT1),a longevity gene,is over-expressed in endometrial carcinoma.

Sirtuin1（SIRT1）是一种长寿基因，在子宫内膜癌中过度表达。

• 发表时间: 2012
• 作者: Matsumoto K;Hirai Y;Furuta R;Takatsuka N;Oki A;Yasugi T;Maeda H;Mitsuhashi A;Fujii T;Kawana K;Iwasaka T;Yaegashi N;Watanabe Y;Nagai Y;Kitagawa T;Yoshikawa H;Asaka R,Miymamoto T,Suzuki A,IshikawaK,Yamada Y,Kobara H,Shiozawa T
• 通讯作者: Asaka R,Miymamoto T,Suzuki A,IshikawaK,Yamada Y,Kobara H,Shiozawa T

Identification of novel genes involved in clear cell carcinogenesis of the overy using retrovirus-based cDNA expression library

使用基于逆转录病毒的 cDNA 表达文库鉴定参与透明细胞癌变的新基因

• 作者: Utsumi F;Kajiyama H;Nakamura K;Tanaka H;Mizuno M;Toyokuni S;Hori M;Kikkawa F.;Shiozawa T,Miyamoto T,Suzuki A,Asaka R.
• 通讯作者: Shiozawa T,Miyamoto T,Suzuki A,Asaka R.

卵巣明細胞癌発生に関与する新規候補遺伝子の機能的スクリーニング

参与卵巢透明细胞癌发生的新候选基因的功能筛选

• 发表时间: 2013
• 作者: 安藤大史;宮本 強;山田 靖;鈴木昭久;浅香亮一;石川香織;小原久典;塩沢丹里
• 通讯作者: 塩沢丹里

正常子宮内膜および子宮内膜癌におけるsirtuin1発現の検討

正常子宫内膜和子宫内膜癌中sirtuin1表达的检测

• 发表时间: 2012
• 作者: 浅香亮一;宮本強;鈴木昭久;鹿島大靖;塩沢丹里
• 通讯作者: 塩沢丹里

Placental mesenchymal dysplasia: chronological observation of placental images during gestation and review of the literature.

胎盘间质发育不良：妊娠期间胎盘图像的时间顺序观察和文献复习。

• DOI: 10.1159/000350661
• 发表时间: 2013
• 期刊: Gynecol Obstet Invest
• 作者: Ohira S;Ookubo N;Tanaka K;Takatsu A;Kobara H;Kikuchi N;Ohya A;Kanai M;Shiozawa T
• 通讯作者: Shiozawa T