Modulation of skin microbiota through basememt membrane

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Skin microbiota of mice deficient for skin basement membrane protein (BMZ-deficient mice) were analyzed. At P1, BMZ-deficient mice had less abundant skin microbiota compared with the control group mice while the quantity of skin bacteria was comparable between BMZ-deficient mice and the control mice at P4. Visualization of skin microbiota using FISH methods revealed that bacterial signals were located mainly in the cornified layers of BMZ-deficient mice and the control mice. The specificity of the probe to detect bacterial signals was confirmed by the negative FISH signals using a probe reacting with fungal species which are not found in skin. Further analyses will be done on microbiota of adult BMZ-deficient mice skin and human epidermolysis bullosa patients' skin.