Reciprocal inhibition loop of protein kinase and phosphatase

细胞分裂需要数百种蛋白质磷酸化，这些蛋白质磷酸化应该在时间和空间上受到调节。本课题主要研究蛋白磷酸酶的功能，而蛋白磷酸酶是迄今为止人们关注较少的蛋白激酶。我们发现多磷酸酶复合物在着丝粒上对细胞分裂中染色体的忠实分离起着重要作用。我们还发现，α-内硫素是一种重要的磷酸酶调节剂，它的酪氨酸残基被磷酸化。我们在试管中证明了残留物的重要性。这些结果暗示了蛋白磷酸酶如何与蛋白激酶结合促进细胞分裂。

Cell division needs hundreds of protein phosphorylation that should be temporally and spacially regulated. This research project focused on functions of protein phosphatases, that attaracted less attention than protein kinases till today. We found that multiple phosphatase complexes paly differertn roles for faithful chromosome segregation on cell division at kinetochores. We also found that alpha-endosulfine, an imprtant regulator for other kind of phosphatase, is phopshorylated on its tyrosine residue. Importance of the residue was shown in a test tube by us. These results imply how protein phosphatases could contribute to the cell division in combination with protein kinases.