Regulation of carotenoid biosynthesis in non- photosynthetic bacteria by light irradiation

11从海洋藻类中分离得到的ShimoA1合成了一种新的类胡萝卜素--2‘-异戊烯基大豆黄质(2’-isopentenyl spx)。蓝光LED照射可增加菌株A1培养基中2‘-异戊烯基Spx的含量。此外，蓝光还能提高类胡萝卜素合成基因crtB、crtI、crty、crtZ的基因表达水平。此外，菌株A1还具有编码戊烯基转移酶和植烯去饱和酶的独特基因，这两个基因可能在菌株A1合成2‘-异戊烯Spx过程中起重要作用。这两个基因也倾向于被蓝色LED上调。这些结果表明，菌株A1是一种独特的细菌，具有受蓝光调控的类胡萝卜素合成途径。

11ShimoA1, which was isolated from a marine alga, synthesized a novel carotenoid,2’-isopentenyl saporxanthin (2’-isopentenyl Spx). Blue LED irradiation increased2’-isopentenyl Spx content in the culture medium of strain A1. Furthermore, blue LED also enhanced mRNA expression level of carotenoid synthetic genes such as crtB、crtI、crtY、crtZ. In addition, strain A1 has unique genes coded prenyltransferase and phytoene desaturase, which are suggested to play an important role for 2’-isopentenyl Spx synthesis in strain A1. Both genes also tended to be up-regulated by blue LED. These results show that strain A1 is a unique bacteria with carotenoid synthetic pathways regulated by blue light.