Regulation of carotenoid biosynthesis in non- photosynthetic bacteria by light irradiation

光照射对非光合细菌类胡萝卜素生物合成的调节

• 批准号: 23658155
• 负责人: HOSOKAWA Masashi
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658155/
• 关键词: 微生物; 光刺激; カロテノイド; 化学合成細菌; 青色光; isopentenylsaproxanthin; Jejuia pallidilutea; 海洋細菌; ゼアキサンチン; Ｆｌａｖｏｂａｃｔｅｒｉａ; Algibacter lectus

11ShimoA1, which was isolated from a marine alga, synthesized a novel carotenoid,2’-isopentenyl saporxanthin (2’-isopentenyl Spx). Blue LED irradiation increased2’-isopentenyl Spx content in the culture medium of strain A1. Furthermore, blue LED also enhanced mRNA expression level of carotenoid synthetic genes such as crtB、crtI、crtY、crtZ. In addition, strain A1 has unique genes coded prenyltransferase and phytoene desaturase, which are suggested to play an important role for 2’-isopentenyl Spx synthesis in strain A1. Both genes also tended to be up-regulated by blue LED. These results show that strain A1 is a unique bacteria with carotenoid synthetic pathways regulated by blue light.

11ShimoA1是从海藻中分离出来的，合成了一种新型类胡萝卜素，2'-异戊烯基皂黄素（2'-isopentenyl Spx）。蓝色 LED 照射增加了菌株 A1 培养基中 2'-异戊烯基 Spx 的含量。此外，蓝色LED还增强了类胡萝卜素合成基因如crtB、crtI、crtY、crtZ的mRNA表达水平。此外，菌株A1具有编码异戊烯基转移酶和八氢番茄红素去饱和酶的独特基因，这表明它们在菌株A1中的2'-异戊烯基Spx合成中发挥重要作用。这两个基因也往往会被蓝色 LED 上调。这些结果表明菌株A1是一种独特的细菌，其类胡萝卜素合成途径受蓝光调节。

项目成果

Production and conversion of functional carotenoids by bacteria

细菌生产和转化功能性类胡萝卜素

• 发表时间: 2012
• 作者: M. Hosokawa;K. Nishida;T. Sawabe;K. Miyashita;Ching T. Hou
• 通讯作者: Ching T. Hou

Jejuia pallidilutea(11ShimoA1株)合成カロテノイドの同定と培養条件の検討

Jejuia pallidirudea（菌株11ShimoA1）合成类胡萝卜素的鉴定及培养条件研究

• 发表时间: 2012
• 作者: M. Hosokawa;K. Nishida;T. Sawabe;K. Miyashita;Ching T. Hou;中嶋正道;細川雅史・西田健太郎・澤辺智雄・宮下和夫・眞岡孝至
• 通讯作者: 細川雅史・西田健太郎・澤辺智雄・宮下和夫・眞岡孝至

Jejuia pallidilutea (11ShimoA1株)合成カロテノイドの同定と培養条件の検討

Jejuia pallidirudea（11ShimoA1株）合成类胡萝卜素的鉴定及培养条件研究

• 发表时间: 2012
• 作者: Takehiko Mochiduki;Noriyoshi Sakai;Toyoko Nakajima;Masamichi Nakajima;細川雅史・西田健太郎・澤辺智雄・宮下和夫・眞岡孝至
• 通讯作者: 細川雅史・西田健太郎・澤辺智雄・宮下和夫・眞岡孝至

Bioconversion of marine carotenoids and their Health functions

海洋类胡萝卜素的生物转化及其保健功能

• 发表时间: 2011
• 作者: M. HOSOKAWA;YJ. Yim;K. Miyashita;CT. Hou
• 通讯作者: CT. Hou

Bacillus megaterium ALA2による fucoxanthinからfucoxanthinolへの変換反応

巨大芽孢杆菌ALA2将岩藻黄质转化为岩藻黄质醇的反应

• 发表时间: 2011
• 作者: Takehiko Mochiduki;Noriyoshi Sakai;Toyoko Nakajima;Masamichi Nakajima;西田健太郎・Ching T. Hou・澤辺智雄・宮下和夫・細川雅史
• 通讯作者: 西田健太郎・Ching T. Hou・澤辺智雄・宮下和夫・細川雅史