頭頚部癌組織におけるcollagenase mRNAの発現と好酸球浸潤に関する研究

头颈癌组织中胶原酶mRNA表达及嗜酸性粒细胞浸润的研究

• 批准号: 08771430
• 负责人: 今西 順久
• 金额: $ 0.51万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771430/
• 关键词: MMP-1(collagenase); 好酸球浸潤; 頭頚部癌

我々の従来の実験結果より,頭頚部癌組織においてmatrix metalloproteinase-1(MMP-1/Collagenase)・は比較的浸潤傾向の弱いpushing marginの腫瘍浸潤形態を示す組織に発現し,特に腫瘍近傍の間質に浸潤した好酸球に強く発現していること,さらに成熟好酸球の活性化因子interleukin-5(IL-5)mRNAが好酸球自身に発現していることから,IL-5が腫瘍組織における好酸球浸潤tumor associated tissue eosinophilia(TATE)を誘導し,好酸球が宿主組織の再構築を目的としてMMP-1を分泌しているという一つの機序が示唆された。今回この系をin vivoのレベルで解明するため,以下の実験を行った。咽頭癌細胞株Hep-2細胞をヌードマウスの皮下に2×10の6乗個移植し,1週間後および2週間後にIL-5を経静脈的に投与して,一定期間後(4〜5週間後)に増殖した腫瘍組織を採取した。新鮮組織からAGPC法によりtotal RNAを抽出し,また4%paraform aldehyde固定標本から連続切片を作成して,好酸性顆粒を特異的に染めるルナ染色,MMP-1 cDNA probeを用いたnortern hybridizationおよびin situ hybridization,抗MMP-1抗体を用いたwestern blotおよび免疫組織化学染色,腫瘍重量測定,増殖曲線の作成を行い,1)好酸球浸潤の程度,2)MMP-1 mRNAの発現,3)MMP-1蛋白の産生,4)腫瘍増殖抑制効果を調べた、IL-5投与群・非投与群の間の比較も含め,各項目の相関関係について統計学的に有意差を検討した。(結果については今後学会等で報告予定である。)

We have come to compare the results of the cancer tissue to the weak pushing margin matrix metalloproteinase-1 (MMP-1/Collagenase) immersion system, which shows that the tissue is visible, and that the acid ball is better than that of the cancer tissue. The activation factor of mature acid globule interleukin-5 (IL-5) mRNA was detected by the acid ball itself, and the acid globule was immersed in tumor associated tissue eosinophilia (TATE) by the tissue of IL-5. The host tissue of acid globule was incubated with acid globule, and the host tissue of acid globulin was used to secrete acid globulus. This time, we are going to tell you what to do. The following is the in vivo line. The pharynx cancer cell line Hep-2 cell line was subcutaneously transplanted for more than 2 × 10 6, 1 year later, 2 weeks later, the IL-5 was injected intravenously after 2 weeks, and the colonization tissue was collected after a regular period (after 4 to 5 years). The new tissue was extracted by AGPC method, and the linked sections of 4%paraform aldehyde fixed headers were made into slides, and the acidic particles were stained with specific in situ hybridization, MMP-1 cDNA probe and anti-MMP-1 antibodies were stained by immunocytochemical staining, weight determination, and colonization test. 1) the degree of acid ball impregnation. 2) MMP-1 mRNA results show that 3) MMP-1 proteins are produced, 4) colonization inhibition results show that there is no significant difference between IL-5 and population, and there is an intentional difference in the statistics of each project. (as a result, we will learn to wait for the final report in the future.)