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培養正常唾液腺細胞に対する遺伝子導入とその移植に関する研究

培养正常唾液腺细胞的基因转移和移植研究

基本信息

批准号：
09771753
负责人：
各務秀明
金额：
$ 1.47万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09771753/
关键词：
唾液腺遺伝子導入培養移植実験培養性線維芽細胞増結因子

项目摘要

1) 培養唾液腺細胞に対する遺伝子導入実験コラゲナーゼ処理にてラット顎下腺細胞を単離し,マイトマイシンCにより増殖能を失わせた3T3細胞とともに平面培養を行った。培養唾液腺細胞に対し,カチオニックリポソーム,レトロウイルスベクターおよびアデノウイルスベクターを用いた遺伝子導入を行フェラーゼとLacZである.遺伝子導入効率は,アデノウイルスベクターが100%(MOI10),リボソーム法では最大10%,レトロウイルスでは10%>であった.遺伝子発現期間はアデノウイルスで4週,リボソーム法では約1週であり,長期間の発現は得られなかった.レトロウイルスベクターについては継続的な発現が得られるものの,選択培養により導入細胞のみを得ることは技術的に困難であり,遺伝子導入後の継代にて十分な増殖を得ることができなかった.2) 遺伝子導入唾液腺細胞の移植に関する研究ヌードマウスを用いた移植実験により培養唾液腺細胞が生着可能であることが明らかとなった。しかし移植された培養唾液腺細胞はin vivoにおいて腺管構造を示さず,均一な細胞集団として存在するため,分泌物や貯留液により長期間の保持は困難であった.安定した移植環境を作り出すためには,増殖活性を持ち形態的にもより分化した細胞を移植することが必要と考えられた.今回唾液腺細胞の増殖や分化を制御する方法の一つとして,細胞増殖因子(bFGF)の培養細胞に与える影響を観察した.bFGFは,唾液腺細胞の分化には影響を与えないものの,増殖を強く促進することが明らかとなった.実験は現在も継続しており,増殖因子の存在下での細胞移植について検討を行っている.

1) the salivary glands were isolated from the cells of the salivary glands, the cells of the salivary glands were isolated, and the cells of the salivary glands were isolated from each other. 1) the salivary glands were isolated from the cells of the salivary glands, and the cells of the salivary glands were isolated from the cells of the salivary glands. the cells of the salivary glands were isolated from the cells of the salivary glands, and the cells of the salivary glands were isolated from the cells of the salivary glands. the cells of the salivary glands were isolated from the cells of the salivary glands, and the cells of the salivary glands were isolated from the cells of the salivary glands. the cells of the salivary glands were isolated from the cells of the lower glands. The salivary glands of the salivary glands were cultured, and the salivary glands were divided into the line, the LacZ, the cells, the glands, the salivary glands and the salivary glands. The rate of admission is 100% (MOI10), the maximum is 10%, the maximum is 10%, and the maximum is 10%. The rate of admission is 10% (MOI10), the maximum is 10%, and the maximum is 10%. During the current period, the cost of the tax is about 4 yuan, and the cost of the tax law is about 1 yuan, and it can be achieved over a long time. If you do not know how to do this, you should choose to improve the performance of the technology. After the introduction of the progeny, the baby was successfully introduced into the salivary glands and transplanted into the salivary glands. 2) the cells of the salivary glands were implanted into the salivary glands. The salivary glands were implanted, in vivo cells, glandular ducts, glands, glands, salivary glands and salivary glands. The environment of diazepam transplantation is used as an environmental indicator, and the colonization activity is maintained in the form of differentiation and differentiation. Cell transplantation is necessary. This time, the methods of controlling the differentiation of salivary gland cells are as follows: cytoplasmic factor (bFGF), cell culture and shadow observation. BFGF, salivary gland cell differentiation, salivary gland Now that there is an increase in the number of reproductive factors, there is a problem of cell transplantation.