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肺胞の発生および修復に付随する肺胞上皮細胞のアポトーシスを調節する遺伝子の検索

寻找调节与肺泡发育和修复相关的肺泡上皮细胞凋亡的基因

基本信息

批准号：
09771987
负责人：
礒濱洋一郎
金额：
$ 1.28万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09771987/
关键词：
肺胞上皮細胞分化アポトーシス

项目摘要

本研究では,肺胞II型上皮細胞から肺胞I型上皮細胞への分化制御機構を解明することを最終日的として,まず,本年度は肺胞II型上皮細胞が分化する際にどのようにして自身の特徴を失うのか,すなわち,脱分化機構に焦点をぼって検討した.本研究を遂行するにあたり,ラット肺胞lI型上皮初代培養細胞をプラスチックプレート上で長時間培養するとサーファクタント分泌能が低下するという知見に基づき,本培養系を肺胞上皮細胞の分化モデルとして応用可能か否かについて種々検討した.その結果,肺胞II型上皮細胞をプラスチックプレート上で長時間培養すると,肺胞II型上皮細胞は脱分化し,肺胞I型上皮細胞へ分化していることが示された.すなわち,II型細胞に特異的な蛋白であるsurfactant proteinのmRNA発現量が経日的に減少する一方,I型上皮細胞に特異的な蛋白の発現は増加した.興味深いことに,この過程でI型細胞様へ分化できないII型細胞は自然にアポトーシスとみなしうる細胞死をおこすことがわかった.(生細胞数の計測,細胞の微細形態観察およびDNAフラグメンテーション(in vitroおよびin situ)などの生化学的知見に基づく.)また,肺胞II型上皮細胞特異的遺伝子の発現調節にprotien kinase Cの活性化および転写因子TTF-1およびHNF3が関与する可能性が示唆された.現在,このアポトーシスを制御する調節蛋白の同定を検討中である.

In this study, the mechanism of differentiation of type II epithelial cells in lung cells was used to understand the most recent days of differentiation of type II epithelial cells in lung cells. this year, the differentiation of type II epithelial cells in lung cells has its own characteristics. The focus of the mechanism of dedifferentiation is the incidence of lung cancer. In this study, the primary culture of type I lung cells was carried out, and the primary culture of type I lung cells was performed in this study. in this study, the primary culture of lung cell type I was used to culture the primary culture of lung cell type I. in this study, the primary culture of lung cell type I cells was used in the primary culture of lung cell type I. The results showed that the lung type II epithelial cells were cultured for a long time, the lung type II epithelial cells were dedifferentiated, and the lung type I epithelial cells were differentiated. The protein of type II cell, the protein of surfactant protein cell, the protein of type I epithelial cell, the protein of type I epithelial cell, the protein of type II cell, the protein of type I epithelial cell, the protein of type I epithelial cell. There is a deep smell, and the process is that type I cell differentiation, type II cell differentiation and cell death. (the number of DNA cells is calculated, and the micromorphology of the cells is examined. The knowledge of biochemistry is very important in the knowledge of biochemistry.). This is the basis for the knowledge of biochemistry. Lung cell type II epithelia is characterized by the activation of protien kinase C, the writing factor TTF-1, HNF3, and possibility. Now, in the system, the protein is the same as the system.