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HPLC-coupled electrospray ionization time of flight mass spectrometer

HPLC 耦合电喷雾电离飞行时间质谱仪

基本信息

批准号：
422116318
负责人：
金额：
--
依托单位：
Ludwig-Maximilians-Universität München (LMU)
依托单位国家：
德国
项目类别：
Major Research Instrumentation
财政年份：
2019
资助国家：
德国
起止时间：
2018-12-31 至无数据
项目状态：
未结题

来源：
https://gepris.dfg.de/gepris/projekt/422116318?language=en
关键词：
HPLC coupled electrospray ionization time

项目摘要

In the context of research programs oriented towards chemical biology, molecular pharmacology and drug delivery, the instrument is to performed routine real-time (i.e. immediate access by multiple users) and accurate chromatographic and mass spectrometric analyses of crude synthetic samples, i.e. prior to purification, as well as the exact mass determination of purified samples. The analysis of crude samples is a critical step to ascertain the presence of the desired product prior to purification as well as to identify possible side reactions. In contrast to other liquid chromatography coupled to mass spectrometry instruments available in the Faculty of Chemistry and Pharmacy at the Ludwig-Maximilians-University in Munich, the new instrument should be adapted to large synthetic molecules, i.e. with m/z up to 20 000 Da. Typical samples include large synthetic foldamers specific to new research in the department of Pharmacy, as well as large peptides, small chemically synthesized proteins, foldamer-peptide and foldamer-protein covalent adducts, and complex lipid/polymer/nucleic acid conjugates. While the need for such analyses was only diffuse throughout the faculty of chemistry and pharmacy, punctual need being expected by many research groups, it became acute after the installation of a new Chair at LMU in September 2017. The instrument is thus to be intensely used by this group while being accessible to the entire Faculty.

在面向化学生物学、分子药理学和药物输送的研究计划的背景下，该仪器将对粗制合成样品进行常规实时(即多用户立即访问)和准确的色谱和质谱分析，即在提纯之前，以及对提纯样品进行准确的质量测定。粗制样品的分析是在提纯之前确定所需产品的存在以及确定可能的副反应的关键步骤。与慕尼黑路德维希-马克西米利安大学化学和药学系现有的与质谱仪联用的其他液相色谱仪器不同，新仪器应适用于大分子合成，即m/z高达20000Da。典型的样品包括药剂系新研究所特有的大的合成的Folamer，以及大的多肽、小的化学合成的蛋白质、Folamer-多肽和Folamer-蛋白质的共价加合物，以及复杂的脂质/聚合物/核酸结合物。虽然对这种分析的需求只分散在整个化学和药学系，许多研究小组都预计需要准时，但在LMU于2017年9月安装了一个新教席后，这一需求变得尖锐起来。因此，该仪器将由该小组密集使用，而整个系系均可使用。