Hybrid mass spectrometer

基于质谱的蛋白质组学已成为分子生物学研究中不可或缺的工具。新型MS技术能够对复杂生物样品中的蛋白质进行高度灵敏和稳健的检测和定量，并允许研究蛋白质复合物和翻译后修饰。混合质谱仪可以被认为是解决这一研究领域基本问题的通用“主力”，因为它可以从复杂的蛋白质混合物中识别、定量和确认蛋白质。所要求的质谱仪将满足生物研究所（IfB）几个研究小组对高分辨率蛋白质组学的需求。该仪器是需要在体内和体外的生化过程的最先进的分析，从自下而上的蛋白质鉴定（半）定量和超灵敏的蛋白质组学。这包括低丰度植物蛋白复合物，翻译后修饰和细胞类型特异性蛋白质组的分析。此外，对于鸟枪法蛋白质组学，理想的质谱系统必须配备高分辨率和高质量精度的分析仪。在选择仪器时，特别注意平衡灵敏度，鲁棒性和速度，以适应IfB对该仪器不同应用的需求。这是IfB的第一台质谱仪，预计将提高该研究所在分子生物学和蛋白质生物化学方面的竞争力。

Mass-spectrometry (MS) based proteomics has become an indispensable tool in molecular biology. Novel MS technologies enable highly sensitive and robust detection and quantification of proteins in complex biological samples, and allow to study protein complexes and posttranslational modifications. A hybrid mass spectrometer can be considered as a versatile 'workhorse' to address fundamental questions in this research area, because it allows to identify, quantify and confirm proteins from complex protein mixtures. The requested mass spectrometer will serve the need for high-resolution proteomics of several research groups at the Institute for Biology (IfB). The instrument is needed for state-of-the art analysis of biochemical processes in vivo and in vitro, ranging from bottom-up protein identification to (semi)quantitative and ultrasensitive proteomics. This includes the analysis of low-abundant plant protein complexes, posttranslational modifications and cell-type specific proteomes. Additionally, for shotgun proteomics, the ideal mass spectrometry system must be equipped with the high resolution and high mass accuracy analyser. When choosing the instrument, special attention was given to balance the sensitivity, robustness and speed, in order to accommodate the needs of the different applications of this instrument at the IfB. This is the first mass spectrometer at the IfB, and it is expected to enhance the competitiveness of the institute in molecular biology and protein biochemistry.