Studies on gamete manipulation (IVF-ET, GIFT, IVF-ETR and Micromanipulation)

配子操作研究（IVF-ET、GIFT、IVF-ETR 和显微操作）

基本信息

批准号：
01480401
负责人：
INOUE Masato
金额：
$ 4.22万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480401/
关键词：
IVF-ER GIFT IVF-ETR ZSPT Micromanipulation Microinjection Zona drilling Subzonal insertion IVFーET 固卵腔内精子注入不妊娠 ZSPT

项目摘要

Superovulation with GnRHa-HMG, ultrasound guided transvaginal oocyte recovery and flexible embryo transfer made it possible to perform IVF-ER on outpatient basis avoiding working on Sunday. The overall pregnancy rate of IVF-ET was 29.0% (357/1231) per ET. IVF-ET was effective for infertility of various kinds of etiology except for male factor infertility with abnormal sperm fertilizing ability (ZSPT*30%). The pregnancy rate was not affected by the day of embryo transfer up to 3 days after insemination. GIFT was applied-with great expectancy, especially for patients with male factor infertility. However, the results were rather disappointing : the pregnancy rate of patients, with abnormal ZSPT (* 30%) was only 7.8%. IVF-ETR was very effective for patients with sperm immobilizing antibody who achieved 56.3% of pregnancy rate per ETR.Clinical feasibility of assisted fertilization by micromanipulation was assessed using non-fertilized oocytes in IVF programme. Both zona piercing and subzonal insertion were safe and efficacious. However, zona drilling with acid solution caused significant damage on the vitelline membrane. Fertilization after sperm microinjection was very poor, and electron microscopic study revealed that the process of pronuclear formation was arrested at stage II in some oocytes. Chemical or electrical activation might be necessary for full developement of pronuclei and subsequent normal cleavage.

用GNRHA-HMG，超声引导的经阴道卵母细胞恢复和柔性胚胎转移的超级排除使得在门诊患者的情况下进行IVF-ER，避免在周日工作。 IVF-ET的总体妊娠率为每ET的29.0％（357/1231）。 IVF-ET对各种病因的不孕症有效，除了具有异常精子受精能力的男性因子不孕（ZSPT*30％）。妊娠率不受胚胎转移日期的影响，直到连授精后3天。适用于巨大的期望，尤其是针对男性因素不育症的患者。但是，结果令人失望：异常ZSPT（* 30％）的患者妊娠率仅为7.8％。 IVF-ETRET对于固定抗体的精子患者非常有效，该抗体的每ETR达到了56.3％的妊娠率。使用非施肥卵母细胞在IVF计划中评估了通过微观屈服的辅助受精的临床可行性。 Zona穿刺和分区插入都是安全有效的。但是，用酸溶液钻孔对卵黄膜造成了重大损害。精子显微注射后的受精非常差，电子显微镜研究表明，在某些卵母细胞中，在II期间停止了核形成的过程。对于全核和随后的正常裂解可能需要化学或电激活。