Studies on gamete manipulation (IVF-ET, GIFT, IVF-ETR and Micromanipulation)

配子操作研究（IVF-ET、GIFT、IVF-ETR 和显微操作）

• 批准号: 01480401
• 负责人: INOUE Masato
• 金额: $ 4.22万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480401/
• 关键词: IVF-ER; GIFT; IVF-ETR; ZSPT; Micromanipulation; Microinjection; Zona drilling; Subzonal insertion; IVFーET; 固卵腔内精子注入; 不妊娠; ZSPT

Superovulation with GnRHa-HMG, ultrasound guided transvaginal oocyte recovery and flexible embryo transfer made it possible to perform IVF-ER on outpatient basis avoiding working on Sunday. The overall pregnancy rate of IVF-ET was 29.0% (357/1231) per ET. IVF-ET was effective for infertility of various kinds of etiology except for male factor infertility with abnormal sperm fertilizing ability (ZSPT*30%). The pregnancy rate was not affected by the day of embryo transfer up to 3 days after insemination. GIFT was applied-with great expectancy, especially for patients with male factor infertility. However, the results were rather disappointing : the pregnancy rate of patients, with abnormal ZSPT (* 30%) was only 7.8%. IVF-ETR was very effective for patients with sperm immobilizing antibody who achieved 56.3% of pregnancy rate per ETR.Clinical feasibility of assisted fertilization by micromanipulation was assessed using non-fertilized oocytes in IVF programme. Both zona piercing and subzonal insertion were safe and efficacious. However, zona drilling with acid solution caused significant damage on the vitelline membrane. Fertilization after sperm microinjection was very poor, and electron microscopic study revealed that the process of pronuclear formation was arrested at stage II in some oocytes. Chemical or electrical activation might be necessary for full developement of pronuclei and subsequent normal cleavage.

GnRHa-HMG超排卵，超声引导经阴道卵母细胞回收和灵活的胚胎移植使得在门诊进行IVF-ER成为可能，避免了周日的工作。IVF-ET的总妊娠率为29.0%（357/1231）。IVF-ET对各种原因的不孕症均有效，但对精子活率异常（ZSPT*30%）的男性因素不孕症无效。受精后3天内，胚胎移植当天的妊娠率不受影响。GIFT的应用具有很大的期望，特别是对于男性因素不育的患者。然而，结果相当令人失望：ZSPT异常（* 30%）的患者的妊娠率仅为7.8%。IVF-ETR对有精子固定抗体的患者非常有效，每次ETR的妊娠率为56.3%。经皮穿刺和皮下置管均安全有效。然而，用酸溶液钻孔会对卵黄膜造成明显的损伤。精子显微注射后的受精率很低，电镜观察显示，部分卵母细胞原核形成过程停滞在第二阶段。化学或电激活可能是必要的原核的充分发展和随后的正常分裂。

项目成果

Inoue, M., Sato, M., Seto, A., Kobayashi, Y., Honda, I., Nomiyama, M. and Fujii, A.: "Electron microscopic observations of human oocytes micro-injected with human spermatozoa." J. Mamma. Ova Res.(1992)

Inoue, M.、Sato, M.、Seto, A.、Kobayashi, Y.、Honda, I.、Nomiyama, M. 和 Fujii, A.：“用人类精子显微注射的人类卵母细胞的电子显微镜观察。”

井上 正人,他: "体外受精卵卵管内移植(IVFーETR)の利点" 日本受精着床学会雑誌. 7. 33-36 (1990)

Masato Inoue 等人：“体外受精和输卵管内移植 (IVF-ETR) 的优点”，日本受精与植入学会杂志，7. 33-36 (1990)。

淡路 英雄,他: "卵子内精子注入の試み" 哺卵研誌. 8. 93-94 (1991)

Hideo Awaji 等人：“卵母细胞内精子注射的尝试”《哺乳动物研究杂志》，8. 93-94 (1991)。

Kobayashi, Y., Honda, I., Inoue, M., Awaji, H., Tsuda, T., Matsuyama, T. and Fujii, A.: "Results of in vitro fertilization-embryo transfer by controlled ovarian hyperstimulation of gonadotropin-releasing hormone against with HMG." J. Fertil. implant (Toky

Kobayashi, Y.、Honda, I.、Inoue, M.、Awaji, H.、Tsuda, T.、Matsuyama, T. 和 Fujii, A.：“通过促性腺激素控制性卵巢过度刺激进行体外受精-胚胎移植的结果

Inoue, I., Awaji, H., Kobayashi, Y., Honda, I. and Fujii, A.: "Micromanipulation of gametes and embryos." Oncologia. 24. 25-28 (1991)

Inoue, I.、Awaji, H.、Kobayashi, Y.、Honda, I. 和 Fujii, A.：“配子和胚胎的显微操作。”