Reconsideration of Sex-steroid Receptor Regulatory Mechanism in the Female Genital Tract

女性生殖道性类固醇受体调节机制的再思考

• 批准号: 05454442
• 负责人: FUJII Shingo
• 金额: $ 4.22万
• 依托单位: Shinsu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454442/
• 关键词: Estrogen and Progesterone Receptor Regulatory Mechanisms; Cell Proliferation; Female Genital Tract; Cyclin Family; Hear Shock Protein 70; Gonadotropin; Endometrial Gland; Endometrial Stroma; サイクリンA,B1,D1,E; cdc2; cdk2,4; 子宮頚部腺上皮; 卵管腺上皮; 子宮頚部扁

The analysis of the expression of sex steroid receptors (ER and PR) and cell proliferation antigens such as Ki-67 and cyclin families (cyclin A,B1, D1, E and cdc2, cdk2) in the tissues of female genital tract (endometrial gland and stroma, myometrial smooth muscle, tubal epithelium, cervical glandular epithelium, cervical squamous epithelium, granulosa cells of the ovary) revealed that the respective tissues/cells in the female genital have tract respective sex-steroid receptor regulatory mechanisms. The classical concept of sex-steroid receptor regulatory mechanism, that estrogen up-regulates both ER and PR inducing cell-proliferation and progesterone down-regulates both ER and PR inducing cell-differentiation, was only applied on the endometrial glands in the functional layr of the endometrium. This concept could not explain the expression of sex-steroid receptors and cell proliferation antigens during the menstrual cycle in the endometrial stromal cells, myometrial smooth muscle cel … More ls, and the glands in the basal layr of the endometrium. In addition, the tubal epithelium, cervical glandular epithelium and cervical squamous spithelium constantly expressed sex-steroid receptors during the menstrual cycle with sporadic cell-proliferations-antigen-positive cells irrespective of the menstrual phase. Moreover, the granulosa cells of the ovary showed the induction of PR under the influence of gonadotropin (LH) . Consequently, it is likely to say that the tissues/cells in the female genital tract have respective sex-steroid receptor regulatory mechanisms. Under the same influence of circulatory sex-steroid hormones, the tissues/cells in the femal genital tract respond differently in order to perform respective functions in the female genital tract. In order to accomplish these different responses to the circulatory sex-steroid, an introduction of the action of various factors in and/or around the respective tissues/cells seem to be necessary. In the endometrium, hear shock protein (HSP) 70 was revealed to act as one of factors controlling different response to the circulatory sex-steroids Moreover, ATL-derived factor (ADF : human thioredoxin) , epidermal growth factor receptors, and c-erb-B2 protein were also suggested as candidates of these factors. In future, through the studies of various growth factors, cytokines, and gonadotropins in the female genital tracts, we might getan important cell biological phenomenon on sex-steroid receptor activating mechanisms which are operating in the respective tissues/cells of the female genital tract. Less

性类固醇激素受体表达分析（ER和PR）和细胞增殖抗原如Ki-67和细胞周期蛋白家族（cyclin A、B1、D1、E和cdc 2、cdk 2）在女性生殖道组织中的表达（子宫内膜腺体和间质，子宫肌层平滑肌，输卵管上皮，宫颈腺上皮，宫颈鳞状上皮，卵巢的颗粒细胞）揭示了女性生殖器中的相应组织/细胞具有各自的性类固醇受体调节机制。雌激素上调ER和PR诱导细胞增殖，孕激素下调ER和PR诱导细胞分化的性类固醇受体调节机制的经典概念仅适用于子宫内膜功能层的子宫内膜腺体。这一概念不能解释月经周期中子宫内膜间质细胞、子宫肌层平滑肌细胞、子宫内膜上皮细胞、子宫内膜细胞和子宫内膜细胞中性类固醇受体和细胞增殖抗原的表达。 ...更多信息 LS和子宫内膜基底层的腺体。此外，输卵管上皮，宫颈腺上皮和宫颈鳞状spithelium不断表达性类固醇受体在月经周期与零星的细胞增殖抗原阳性细胞，无论月经期。此外，卵巢颗粒细胞在促性腺激素（LH）的影响下表现出PR的诱导作用。因此，可以说女性生殖道中的组织/细胞具有各自的性类固醇受体调节机制。在循环性类固醇激素的相同影响下，女性生殖道中的组织/细胞做出不同的反应，以在女性生殖道中执行各自的功能。为了实现对循环性类固醇的这些不同反应，在相应组织/细胞中和/或周围引入各种因子的作用似乎是必要的。在子宫内膜中，热休克蛋白（HSP 70）是控制对循环性激素不同反应的因素之一。此外，ATL衍生因子（ADF：人硫氧还蛋白）、表皮生长因子受体和c-erb-B2蛋白也被认为是这些因素的候选者。通过对女性生殖道中各种生长因子、细胞因子和促性腺激素的研究，我们可能会发现一种重要的细胞生物学现象，即性类固醇受体激活机制在女性生殖道的相应组织/细胞中起作用。少

项目成果

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