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Experimental study of the effect of T cell on alveolar bone resorption induced by bacterial endotoxin

T细胞对细菌内毒素诱导的牙槽骨吸收影响的实验研究

基本信息

批准号：
05454519
负责人：
HARA Yoshitaka
金额：
$ 4.54万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454519/
关键词：
Bone resorption T cell Nude mouse SCID mouse T細胞病理組織学的検索

项目摘要

Periodontal disease are caused by bacterial infection and some immune cells locally infiltrated against it. There cells secrete cytokines which have been reported to act not only as regulatory substances for immune responses, but mediators of tissue destruction. In fact, there have been many reports which suggest the relation between some cytokines secreted from T cell, a mediator of immune responses, and alveolar bone resorption. However, it has been unclear that T cells truly relate with periodontal tissue destruction. So in first study, alveolar bone resorption induced by every 48 h injections of E.coli endotoxin was investigated in normal mice, T cell deficient nude mice and SCID mice congenitally lacking both T and B cells. In addition, CD4 and/or CD8 depleted mice by the administration of anti-CD4 and/or anti-CD8 monoclonal antibodies were used in this study. In normal mice, alveolar bone resorption was observed after 4 times injection, and after 7 times in nude mice and 10 times in SCID mice. T cell depleted mice, especially CD4 depleted mice, also revealed the weaker and delayd bone resorption. These findings strongly suggested the relation between T cells and bone resorption. So in second experiment, mice transfered with T cells were established and injected with endotoxin as same as in first study. After 4 times injection, these mice showed the weak but obvious bone resorption similarly in normal mice. Based on these results, T cells closely related the bone resorption induced by endotoxin and further study should be investigated on cytokine production by T cells.

牙周病是由细菌感染引起的局部免疫细胞浸润引起的疾病，这些细胞分泌的细胞因子不仅是免疫应答的调节物质，而且是组织破坏的介质。事实上，已有许多研究表明，T细胞分泌的某些细胞因子与牙槽骨吸收有关。然而，目前尚不清楚T细胞是否真的与牙周组织破坏有关。因此，在第一项研究中，在正常小鼠、T细胞缺陷裸鼠和先天性缺乏T和B细胞的SCID小鼠中研究了每48小时注射大肠杆菌内毒素诱导的牙槽骨吸收。此外，在本研究中使用了通过给予抗CD 4和/或抗CD 8单克隆抗体而去除CD 4和/或CD 8的小鼠。正常小鼠4次注射后可见牙槽骨吸收，裸鼠7次注射后可见牙槽骨吸收，SCID小鼠10次注射后可见牙槽骨吸收。T细胞减少的小鼠，尤其是CD 4减少的小鼠，也显示出较弱的和延迟的骨吸收。这些结果有力地表明T细胞与骨吸收之间的关系。因此，在第二个实验中，建立了T细胞转移的小鼠，并与第一个研究相同地注射内毒素。注射4次后，这些小鼠表现出与正常小鼠相似的微弱但明显的骨吸收。因此，T细胞与内毒素诱导的骨吸收密切相关，T细胞产生细胞因子的机制有待进一步研究。