A study for establishment of judging periodontal disease activity

牙周病活动性判断标准建立的研究

• 批准号: 08672199
• 负责人: HARA Yoshitaka
• 金额: $ 1.47万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672199/
• 关键词: IFN-gamma; IL-4; periodontal disease; Immunohistological study; 歯肉溝浸出液; サイトカイン; IL-1; TNF-α

The purpose of this study was to clarify the role of IFN-gamma and IL-4 in periodontal disease. Gingiva was biopsied at tooth extraction or periodontal surgery after examining clinical parameters. Serial paraffin sections were obtained and every five sections were stained immunohistologically with anti-IFN-gamma or IL-4 monoclonal antibody. The specimens were divided into three groups ; no treatment (NT) obtained at tooth extraction, treatment 1 (T1) obtained at periodontal surgery with deep pocket, and treatment 2 (T2) with shallow pocket. Then the number of IFN-gamma bearing cells was highest in NT and that in T2 was the next. When the specimens were grouped with bleeding on probing (Bop), the number of IFN-gamma bearing cells was higher in Bop+. Furthermore, the number of IFN-gamma bearing cells was higher in the gingiva with strong inflammatory cell infiltration than that with weak one. On the other hand, IL-4 bearing cells showed opposite tendency. These findings suggested that IFN-gamma is related with activation of periodontal disease and IL-4 plays the suppressive role in periodontal disease activity.

本研究旨在阐明干扰素-γ和白介素4在牙周病中的作用。在检查临床参数后，在拔牙或牙周手术时进行牙龈活组织检查。取连续石蜡切片，每5张切片用抗干扰素-γ或IL-4单抗进行免疫组织化学染色。将标本分为三组：拔牙时不处理(NT)，深袋牙周手术时处理1(T1)，浅袋处理2(T2)。其次为NT组，T2组次之，NT组最多。按探诊出血(BOP)分组时，BOP+的干扰素-γ阳性细胞数较多。炎症细胞浸润程度较强的牙周组织中，干扰素-γ的阳性细胞数明显高于炎症细胞浸润程度较弱的牙周组织。而携带IL-4的细胞则表现出相反的趋势。提示干扰素-γ与牙周病的活动性有关，IL-4在牙周病的活动性中起抑制作用。