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Immunohistlogical study of mouse periodontal burst model

小鼠牙周爆裂模型的免疫组织学研究

基本信息

批准号：
13672192
负责人：
HARA Yoshitaka
金额：
$ 2.3万
依托单位：
NAGASAKI University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672192/
关键词：
LPS Bone resorption Interferron-γ IL-1 β Osteoclast LPS Interferon-γ IL-1β

项目摘要

It has been reported that bacterial lipopolysaccharide (LPS) induces alveolar bone resorption and that the host immune system especially activated T cells, plays a crucial role in osteoclastogenesis. On the other hand, interferon-gamma (IFN-γ), which is produced by activated T cells, suppresses bone resorption both in vitro and in vivo. Thus, the question arises as to whether or not IFN-γ production increases with increasing bone resorption. We previously demonstrated that repeated injections of Escherichia coli LPS into mouse gingiva causes osteoclast formation in alveolar bone. In the present study we observed changes in the IFN-γ production of infiltrating cells in concurrence with bone resorption. Mice were repeatedly injected with 5μg LPS 26times every 48h. After the 16th injection when the alveolar bone resorption reached a plateau, the concentration of LPS was altered (25μg LPS or PBS alone). The level of active resorption became significantly elevated, and the number of IFN-γ- and interleukin-1 beta (IL-1 β)- bearing cells also increased significantly, in relation to bone resorption within the 25γg LPS injected group. On the other hand, few tartrate-resistant acid phosphatase positive cells, or IFN-γ- and IL-1 β- bearing cells, were seen in the PBS injected group. These results suggest that alteration in IFN-γ-bearing cells might play arole in counterbalancing LPS induced bone resorption resulting from osteoclast activating cytokines such as IL-1 β.

细菌脂多糖（LPS）可诱导牙槽骨吸收，而宿主免疫系统尤其是活化的T细胞在破骨细胞生成中起着至关重要的作用。另一方面，由活化的T细胞产生的干扰素-γ（IFN-γ）在体外和体内均抑制骨吸收。因此，问题是IFN-γ的产生是否随着骨吸收的增加而增加。我们以前证明，反复注射大肠杆菌脂多糖到小鼠牙龈导致破骨细胞形成的牙槽骨。在本研究中，我们观察了在骨吸收的同时，浸润细胞的IFN-γ产生的变化。每48 h重复注射LPS 5μg，共26次。第16次注射后，当牙槽骨吸收达到平台时，改变LPS的浓度（25μg LPS或单独的PBS）。在25 μ g LPS注射组中，与骨吸收相关的主动吸收水平显著升高，并且携带IFN-γ和白细胞介素-1 β（IL-1 β）的细胞的数量也显著增加。另一方面，在PBS注射组中观察到很少的抗酒石酸盐酸性磷酸酶阳性细胞或携带IFN-γ和IL-1 β的细胞。这些结果表明，IFN-γ-承载细胞的改变可能在抵消LPS诱导的破骨细胞激活细胞因子如IL-1 β引起的骨吸收中起作用。