The regulation of replication of ATL cells in SCID mouse

SCID小鼠ATL细胞复制的调控

• 批准号: 05670428
• 负责人: OKAYAMA Akihiko
• 金额: $ 1.34万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670428/
• 关键词: HTLV-I; SCID mouse; MT-2; 抗アシアロGM-1抗体; NK活性

Effect of anti-asialo GM-1 antibody (AAGM) treatment on the graftment of severe combined immunodeficiency (SCID) mice with human T-cell leukemia virus type-I (HTLV-I) infected human T cells was studied. The frequency of forming tumors of various size at the inoculation site was significantly more in 16/18 (89%) of AAGM treated mice than in 16/26 (62%) of AAGM untreated mice (p<0.05), when an HTLV-I transformed human T-cell line, MT-2, was inoculated. When another HTLV-I transformed human T-cell line, HUT102, was inoculated, the frequency of forming tumors of was 7/10 (70%) in AAGM untreated mice, which was close to that of MT-2. The effect of AAGM treatment was obvious to promote the development of tumors in the early stage (less than 3 weeks), suggesting that an immediate natural killer (NK) reaction after inoculation of the cells might be important for the prevention of the tumor development. The characteristics of tumor cells, such as surface phenotypes and clonality, were same as those of MT-2 cells. Two of the 23 AAGM treated mice, which received peripheral blood mononuclear cells from 5 patients with ATL,developed tumors at the in jection site. In addition, it was observed that the HTLV-I could be detected in various organs by polymerase chain reaction, when the peripheral blood lymphocytes of ATL patients and of a healthy carrier were inoculated in AAGM treated mouse. These results clearly suggest that elimination of the NK function by AAGM treatment is important, although this is not always necessary for engrafting HTLV-I transformed cells in SCID mice.

研究了抗去唾液酸 GM-1 抗体 (AAGM) 治疗对感染人 T 细胞白血病病毒 I 型 (HTLV-I) 的人 T 细胞移植到严重联合免疫缺陷 (SCID) 小鼠的影响。当接种 HTLV-1 转化的人 T 细胞系 MT-2 时，在 AAGM 处理的小鼠中，16/18 (89%) 的 AAGM 处理的小鼠在接种部位形成各种大小肿瘤的频率显着高于 16/26 (62%) AAGM 未处理的小鼠 (p<0.05) (p<0.05)。当接种另一种HTLV-I转化的人T细胞系HUT102时，在AAGM未处理的小鼠中，形成肿瘤的频率为7/10(70％)，与MT-2接近。 AAGM治疗在早期（小于3周）对肿瘤的发展有明显的促进作用，这表明接种细胞后立即发生的自然杀伤（NK）反应可能对预防肿瘤的发展具有重要意义。肿瘤细胞的表面表型和克隆性等特征与MT-2细胞相同。 23 只 AAGM 治疗的小鼠接受了 5 名 ATL 患者的外周血单核细胞，其中两只在注射部位出现了肿瘤。此外，当将ATL患者和健康携带者的外周血淋巴细胞接种到AAGM处理的小鼠中时，通过聚合酶链反应观察到，可以在各个器官中检测到HTLV-I。这些结果清楚地表明，通过 AAGM 治疗消除 NK 功能很重要，尽管这对于在 SCID 小鼠中移植 HTLV-I 转化细胞而言并不总是必需的。

项目成果

Ishihara S.et al: "Enhanced engraftment of HTLV-I infected human T cells in severe combined immunodeficiency mice by anti-asialo GM-I antibody treatment" Microbiology and Immunology. 40. 39-44 (1996)

Ishihara S.等人：“通过抗去唾液酸GM-I抗体治疗，增强HTLV-I感染的人类T细胞在严重联合免疫缺陷小鼠中的植入”微生物学和免疫学。

Ishihara S.et al.: "Enhanced engraftment of HTLV-I infected human T cells in severe combined immunodeficiency mice by antiasialo GM-I antibody treatment." Microbiology and Immunology. 40. 39-44 (1996)

Ishihara S.等人：“通过抗唾液酸 GM-I 抗体治疗，增强了严重联合免疫缺陷小鼠体内 HTLV-I 感染的人类 T 细胞的植入。”