The regulation of replication of ATL cells in SCID mouse

本文研究了抗去唾液酸GM-1抗体（AAGM）对人T细胞白血病病毒Ⅰ型（HTLV-Ⅰ）感染的人T细胞移植到重症联合免疫缺陷（SCID）小鼠体内的影响。当接种HTLV-I转化的人T细胞系MT-2时，在接种部位形成各种大小的肿瘤的频率在16/18（89%）的AAGM处理的小鼠中显著高于16/26（62%）的AAGM未处理的小鼠（p<0.05）。当接种另一种HTLV-I转化的人T细胞系HUT 102时，在AAGM未处理的小鼠中形成肿瘤的频率为7/10（70%），这接近MT-2的频率。AAGM处理对早期（小于3周）肿瘤的发展有明显的促进作用，表明细胞接种后的立即自然杀伤（NK）反应对于预防肿瘤发展可能是重要的。肿瘤细胞的表面表型和克隆性与MT-2细胞相同。23只AAGM处理的小鼠中有两只接受了来自5名ATL患者的外周血单核细胞，在注射部位发生了肿瘤。此外，当ATL患者和健康携带者的外周血淋巴细胞接种到AAGM处理的小鼠中时，通过聚合酶链反应可以在各种器官中检测到HTLV-I。这些结果清楚地表明，通过AAGM处理消除NK功能是重要的，尽管这对于在SCID小鼠中移植HTLV-I转化的细胞并不总是必需的。

Effect of anti-asialo GM-1 antibody (AAGM) treatment on the graftment of severe combined immunodeficiency (SCID) mice with human T-cell leukemia virus type-I (HTLV-I) infected human T cells was studied. The frequency of forming tumors of various size at the inoculation site was significantly more in 16/18 (89%) of AAGM treated mice than in 16/26 (62%) of AAGM untreated mice (p<0.05), when an HTLV-I transformed human T-cell line, MT-2, was inoculated. When another HTLV-I transformed human T-cell line, HUT102, was inoculated, the frequency of forming tumors of was 7/10 (70%) in AAGM untreated mice, which was close to that of MT-2. The effect of AAGM treatment was obvious to promote the development of tumors in the early stage (less than 3 weeks), suggesting that an immediate natural killer (NK) reaction after inoculation of the cells might be important for the prevention of the tumor development. The characteristics of tumor cells, such as surface phenotypes and clonality, were same as those of MT-2 cells. Two of the 23 AAGM treated mice, which received peripheral blood mononuclear cells from 5 patients with ATL,developed tumors at the in jection site. In addition, it was observed that the HTLV-I could be detected in various organs by polymerase chain reaction, when the peripheral blood lymphocytes of ATL patients and of a healthy carrier were inoculated in AAGM treated mouse. These results clearly suggest that elimination of the NK function by AAGM treatment is important, although this is not always necessary for engrafting HTLV-I transformed cells in SCID mice.