Experimental Research of Pathophisiology and Treatment of Cerebral Vasospasm in Rabbit Model with Neurologic Symptoms, - The Effect of Elastase Substance Administration -

具有神经症状的家兔模型脑血管痉挛的病理学和治疗的实验研究 - 弹性蛋白酶物质给药的效果 -

基本信息

批准号：
62480306
负责人：
ENDO Shunro
金额：
$ 2.18万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

*Experimental model of symptomatic vasospasm and investigations of cerebral blood flow, sensory evoked potetial (SEP) and pathologic findings : The common carotid arteries were ligated bilaterally 2 weeks before twice induction of subarachnoiu hemorrhage in rabbits. Rabbits showed a progressing neurologic deficit that was worst on the fourth or fifth day after the subarachnoid hemorrhage. On the fourth day after the subarachnoid hemorrhage, the cortical blood flow showed 20-30% reduction in symptomatic rabbits in comparison with normal ones. SEP also showed nearly changes. In pathological investigations, Characteristic findings of vasospasm were observed on the basilal artery of symptomatic rabbits. However, the infraction changes of cerbral tissues were minimum.Presumably, the rabbits became symptomatic because they no longer had collateral blood flow to compensate for the reduced blood flow in the basilar artery after subarachnoid hemorrhage. This model of symptomatic vasospasm may be beneficial for future studies. We are now trying to make a new model with more serious symptoms.*The effect of elastase administration : For the protection of symptomatic vasospasm, the effect of elastase substance that prevents the damage of elastic layers was expected. In this seriese, the administration of elastase substances in various amounts and methods were experimented. The results were almost negative. The combined treatment with other materials are investigating now.

*症状性血管痉挛的实验模型以及对脑血流，感觉诱发的盆栽（SEP）和病理学发现的研究：在兔子中两次诱导次动脉出血。兔子表现出进展的神经系统缺陷，在亚蛛网膜下腔出血后的第四天或第五天最坏。在亚蛛网膜下腔出血后的第四天，与正常兔相比，皮质血流降低了20-30％。 SEP也显示出几乎变化。在病理研究中，在有症状的兔子的底动脉上观察到了血管痉挛的特征发现。然而，Cerbral组织的违规变化是最小的。明显，这些兔子变得有症状，因为它们不再具有副血液流动来补偿亚蛛网膜下腔出血后基底动脉的降低。这种有症状的血管痉挛模型可能对未来的研究有益。现在，我们正在尝试制作一个具有更严重症状的新模型。在本系列中，实验了各种量和方法的弹性酶物质。结果几乎为负。与其他材料的合并处理现在正在研究。