Identification and Transduction Mechanisms in Chemoreceptor Cells
化学感受器细胞的识别和转导机制
基本信息
- 批准号:63480496
- 负责人:
- 金额:$ 4.42万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Taste receptor mechanisms for salts, sweet substances and umami substances were investigated using the canine taste system. It was found that amiloride suppresses the responses to salts including NaCl, KCI and NH_4Cl. This suppression of the salt response was explained by adsorption of amiloride cation on the salt receptor sites. The responses to sugars were greatly enhanced by the presence of salts. The enhancement was explained by an increase in cooperativity between sugar receptors. It was found that canine taste system responds sensitively to umami substances. The response to monosodium glutamate (MSG) was suppressed by amiloride, but the response to disodium guanylate (GMP) or that induced by synergism between MSG and GMP was not suppressed by amiloride. These results suggested that the response to MSG is composed of salt response and the response to GMP alone or that induced by synergism is composed of umami response.2. Olfactory receptor mechanisms were investigated using the turtle olfactory system. The cross adaptation method was applied to the turtle olfactory system and discrimination of various odorants was quantitatively measured. This method opened the way to study the structure-activity relationship in olfactory system. Perfusion of the turtle olfactory system with salt free solution unchanged the olfactory responsed to various odorants, suggesting that cation channels located at olfactory cilia do not contribute to in vivo olfactory system. It was found that liposomes composed of phosphatidylcholine (PC) and phosphatidylserine (PS) respond sensitively to various odorants. The sensitivity of the liposomes (PS/PC = 0.2) to amyl acetate was much higher than that of the turtle or the frog olfactory system.
1. 利用犬的味觉系统研究了盐、甜物质和鲜味物质的味觉受体机制。结果表明,阿米洛利抑制了其对NaCl、KCI和NH_4Cl等盐的反应。这种对盐反应的抑制可以通过在盐受体位点上吸附氨酰化阳离子来解释。盐的存在大大增强了对糖的反应。这种增强可以用糖受体之间的协同性增加来解释。研究发现,犬的味觉系统对鲜味物质反应敏感。阿米洛利可抑制小鼠对味精(MSG)的反应,但对鸟苷酸二钠(GMP)或味精与GMP协同作用诱导的反应不受阿米洛利的抑制。这些结果表明,对味精的反应是由盐反应组成的,而对GMP单独或协同作用诱导的反应是由鲜味反应组成的。利用龟的嗅觉系统对嗅觉受体机制进行了研究。将交叉适应方法应用于龟的嗅觉系统,定量测定了龟对各种气味的辨别能力。该方法为研究嗅觉系统的构效关系开辟了道路。向龟嗅觉系统灌注无盐溶液后,其嗅觉系统对各种气味的反应没有改变,这表明位于嗅纤毛的阳离子通道对体内嗅觉系统没有贡献。发现由磷脂酰胆碱(PC)和磷脂酰丝氨酸(PS)组成的脂质体对各种气味有敏感反应。脂质体(PS/PC = 0.2)对乙酸戊酯的敏感性远高于海龟和青蛙的嗅觉系统。
项目成果
期刊论文数量(80)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kenzo Kurihara: "Transduction mechanisms in chemoreception" Biosignal Transduction Mechanisms. 181-205 (1989)
Kenzo Kurihara:“化学感受的转导机制”生物信号转导机制。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kiyonori Yoshii and Kenzo Kurihara: "Inward rectifier produced by Xenopus oocytes injected with mRNA extracted from carp olfactory epithelium." Synapse. 3. 234-238 (1989)
Kiyonori Yoshii 和 Kenzo Kurihara:“非洲爪蟾卵母细胞注射从鲤鱼嗅觉上皮中提取的 mRNA 后产生的内向整流器。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kurihara,K.: "Physiology and Pharmacology of Transmembrane Signaling" Excerpta Medica, 404 (1989)
Kurihara,K.:“跨膜信号传导的生理学和药理学”医学摘录,404 (1989)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yusuke,Kato/Kenzo,Kurihara: Comparative Biochemistry and Physiology. 92A. 107-109 (1989)
加藤佑介/栗原贤三:比较生物化学和生理学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kumazawa,T.: "Large enhancement of canine taste nerve responses to sugars by salts" Journal of General Physiology. (1990)
Kumazawa,T.:“盐大大增强了犬类味觉神经对糖的反应”普通生理学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KURIHARA Kenzo其他文献
KURIHARA Kenzo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KURIHARA Kenzo', 18)}}的其他基金
Factory Automation Control System Designing Method Aiming High reliability and High Productivity of Software
以软件高可靠性和高生产率为目标的工厂自动化控制系统设计方法
- 批准号:
15560227 - 财政年份:2003
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
RARE KEY SUBSTANES OLFACTORY SENSE : IDETIFICATION OF HOME STREAM SUBSTANCES IN SALMON
稀有关键物质嗅觉:三文鱼中原生物质的识别
- 批准号:
12045257 - 财政年份:2000
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular discrimination and transduction in singe olfactory and vomeronasal receptor cells
单一嗅觉和犁鼻受体细胞的分子辨别和转导
- 批准号:
09480169 - 财政年份:1997
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of safety inhibitor which selectively masks receptor sites for bitter substances
开发选择性掩盖苦味物质受体位点的安全抑制剂
- 批准号:
09557185 - 财政年份:1997
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
New development of transduction mechanism in chemical senses
化学意义上转导机制的新进展
- 批准号:
07308049 - 财政年份:1995
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
A new transduction mechanism of chemoreceptor cells and cloning of receptor protein
化学感受细胞新转导机制及受体蛋白克隆
- 批准号:
07457533 - 财政年份:1995
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Masking of bitter taste and smell of drugs by protein-lipid complex prepared from materials for foods
用食品材料制备的蛋白质-脂质复合物掩盖药物的苦味和气味
- 批准号:
06557119 - 财政年份:1994
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Cloning of genes encoding putative taste receptor proteins
编码假定味觉受体蛋白的基因的克隆
- 批准号:
05454627 - 财政年份:1993
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Preparation of artificial membrane sensor which responds to various odorants
响应多种气味的人工膜传感器的制备
- 批准号:
01870096 - 财政年份:1989
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
Mechanisms of stimuli discrimination and transduction in gustatory and olfactory cells
味觉和嗅觉细胞的刺激辨别和转导机制
- 批准号:
60480457 - 财政年份:1985
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
The Receptor Basis for Serotonergic Modulation of Olfaction Across Multiple BrainAreas
跨多个脑区嗅觉的血清素调节的受体基础
- 批准号:
10403591 - 财政年份:2018
- 资助金额:
$ 4.42万 - 项目类别:
Establishment of receptor-based odor sensing technology with high sensitivity using accessory proteins of insect olfaction
利用昆虫嗅觉辅助蛋白建立基于受体的高灵敏度气味传感技术
- 批准号:
18K05671 - 财政年份:2018
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Receptor Basis for Serotonergic Modulation of Olfaction Across Multiple BrainAreas
跨多个脑区嗅觉的血清素调节的受体基础
- 批准号:
10424907 - 财政年份:2018
- 资助金额:
$ 4.42万 - 项目类别:
Study on molecular biological mechanism of regeneration of olfactory receptor neurons and regeneration medicine of olfaction
嗅觉受体神经元再生的分子生物学机制及嗅觉再生药物研究
- 批准号:
18390458 - 财政年份:2006
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
OLFACTION: MOLECULAR CLONING OF A HISTAMINE RECEPTOR
嗅觉:组胺受体的分子克隆
- 批准号:
3035401 - 财政年份:1990
- 资助金额:
$ 4.42万 - 项目类别:
Receptor Proteins in Vertebrate Olfaction
脊椎动物嗅觉中的受体蛋白
- 批准号:
8820690 - 财政年份:1989
- 资助金额:
$ 4.42万 - 项目类别:
Standard Grant
OLFACTION: MOLECULAR CLONING OF A HISTAMINE RECEPTOR
嗅觉:组胺受体的分子克隆
- 批准号:
3035399 - 财政年份:1989
- 资助金额:
$ 4.42万 - 项目类别: