Preparation of artificial membrane sensor which responds to various odorants

响应多种气味的人工膜传感器的制备

• 批准号: 01870096
• 负责人: KURIHARA Kenzo
• 金额: $ 3.46万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01870096/
• 关键词: Lipid bilayr; Odor sensor; Phosphatidilserine; eptide; Olfactory organ; Lipid composition; Floral odor; Minty odor; においセンサ-; フォスファチジルセリン; 脂質膜; ニオイ; センサ-; 膜電位; リポソ-ム; 膜流動性; コンカナバリンA; 嗅覚; 人工膜; 応答感度

The conditions to increase the sensitivities of liposomes to odorants were examined. The minimum concentration of amyl acetate to induce the membrane potential changes (threshold) in phosphatidylcholine (PC) liposomes was about 10^<-4>M and addition of 10 or 20% phospatidylserine (PS) lowered the threshold to about 10^<-9>M, which was lower than the thresholds for amyl acetate in the turtle and frog olfactory systems. Similar to amyl acetate, addition of PS to PC greatly lowered the threshold for ionone. The membrane fluidity change of the liposomes in response to odorants occurred at similar concentration region where the membrane potential changes occurred.The effects of addition of proteins and peptides on selectivity of liposomes to odorants were also examined. Incorporation of Con A to PS/PC = 0.2 liposomes greatly increased the response to citral and decreased that to amyl acetate. Incorporation of Leu-Leu to PS/PC = 0.2 liposomes increased the response to odorants having floral odor. It was concluded that quality of odor can be identified by response pattern of liposomes having different composition.

考察了提高脂质体对气味敏感度的条件。磷脂酰胆碱(PC)脂质体膜电位变化的最低浓度(阈值)约为10~(-4)M，加入10%或20%的磷脂酰丝氨酸(PS)可使膜电位的阈值降低到约10~(-9)M，低于甲鱼和蛙嗅觉系统中的阈值。与乙酸戊酯类似，PS的加入大大降低了紫罗兰酮的阈值。在发生膜电位变化的相似浓度范围内，脂质体对气味的响应发生了膜流动性的变化，并考察了蛋白质和多肽的添加对脂质体对气味选择性的影响。在PS/PC=0.2的脂质体中掺入刀豆蛋白A可显著提高其对柠檬醛的反应性，降低对乙酸戊酯的反应性。将Leu-Leu掺入PS/PC=0.2脂质体中，可增强对有花香气味的气味的反应。结论：不同组成的脂质体对气味的响应模式不同，可对气味质量进行鉴别。

项目成果

TAKAYUKI SHOJI: "Sensitivity and Transduction Mechanisms of Responses to General Odorants in Turtle Vomeronasal System." J.Gen.Physiol.98. 909-919 (1991)

TAKAYUKI SHOJI：“海龟犁鼻系统对一般气味反应的敏感性和转导机制。”

Kurihra,K.: "Physiology and Pharmacology of Transmembrane Signaling" Excerpta Medica, 404 (1989)

Kurihra,K.：“跨膜信号转导的生理学和药理学”医学摘录，404 (1989)

TAKAYUKI SHOJI: "Sensitivity and Transduction Mechanisms of Responses to General Odorants in Turtle Vomeronasal System." J.Gen.Physiol. 98. 909-919 (1991)

TAKAYUKI SHOJI：“海龟犁鼻系统对一般气味反应的敏感性和转导机制。”

榎本 秀一: "味およびにおいと膜" 蛋白質核酸酵素. 36. 1779-1789 (1991)

Shuichi Enomoto：“味道、气味和膜”蛋白质核酸酶。36。1779-1789（1991）

Kurihra,K.: "Molewlar mechanisms of taste and odor reception" Comments on Aqricultural and Food Chemistry. 2. 1-50 (1989)

Kurihra,K.：“味道和气味接收的分子机制”对农业和食品化学的评论。