Molecular discrimination and transduction in singe olfactory and vomeronasal receptor cells

单一嗅觉和犁鼻受体细胞的分子辨别和转导

• 批准号: 09480169
• 负责人: KURIHARA Kenzo
• 金额: $ 8.32万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480169/
• 关键词: olfactory cell; pheromone; turtle; frog; rat; vomeronasal call; IP3; cAMP; サイクリックAMP; フェロモン受容細胞; 尿; イノシトールトリスリン酸; 二次メッセンジャー; 性成熟; 嗅線毛; 鋤鼻器; イノシトールトリスソン酸

In order to clarify the odor specificity of a single olfactory cell, various odorants were applied to single olfactory cells of turtle and frog. The results indicated that a single olfactory cell responds to various odorants. In order to examine roles of cyclic GMP in olfactory transduction, cyclic GMP was injected to turtle olfactory cells. The injection induced inward currents. We concluded that the inward currents were induced via cyclic AMP-dependent channel, indicating that pathway specific to cyclic GMP does not exit in turtle olfactory cells. In order to clarify the transduction mechanism of vomeronasal receptor cells, we applied urine, which contains pheromone(s), to rat female vomeronasal cells. Single vomeronasal cell responded only to one species of urine, indicating that a single vomeronasal cell has high specificity. We identified that active components in urine are peptides with molecular weight below 5000. We also tried to identify second messenger in vomeronasal cells. Injection of cyclic AMP to vomeronasal cells induced no response, but injection of inositol trisphosphate (IP3) induced inward currents. Application of inhibitors for generation of IP3 or a blocker of IP3-gated channel blocked the inward currents, suggesting that IP3 is a second messenger in vomeronasal cells.

为了明确单个嗅细胞的气味特异性，将不同的气味物质应用于甲鱼和青蛙的单个嗅细胞。结果表明，单个嗅觉细胞对不同的气味有反应。为了研究环状GMP在嗅觉传导中的作用，将环状GMP注入甲鱼嗅觉细胞。这种注入产生了内向电流。我们的结论是，内向电流是通过环状AMP依赖的通道诱导的，这表明在甲鱼嗅觉细胞中不存在环状GMP特异的通路。为了阐明犁鼻受体细胞的转导机制，我们将含有信息素的尿液(S)作用于雌性犁鼻细胞。单个犁鼻细胞只对一种尿液有反应，表明单个犁鼻细胞具有很高的特异性。我们鉴定出尿液中的活性成分是分子量在5000以下的多肽。我们还试图确定犁鼻细胞中的第二信使。向犁鼻细胞注射环磷酸腺苷无反应，注射三磷酸肌醇(IP3)则产生内向电流。应用IP3抑制剂或IP3门控通道阻断剂阻断内向电流，提示IP3是犁鼻细胞的第二信使。

项目成果

Kashiwayanagi, M.: "Long periodrecording of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb"Brain Ras. Protocols. 1. 287-291 (1997)

Kashiwayanagi, M.：“长期记录海龟主嗅球和副嗅球的嗅觉和犁鼻刺激物引起的波”Brain Ras。

K. Inamura, M. Kashiwayanagi and K. Kurihara: "Inositol 1,4,5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices"Chem. Senses. 22. 93-104 (1997)

K. Inamura、M. Kashiwayanagi 和 K. Kurihara：“肌醇 1,4,5-三磷酸诱导大鼠犁鼻感觉切片中受体神经元的反应”Chem.

M.Kashiwayanagi: "Concentration and membrane fluidity dependence of odor discrimination in the turtle olfactory system." Chem.Sneses.22. 553-563 (1997)

M.Kashiwayanagi：“海龟嗅觉系统中气味辨别的浓度和膜流动性依赖性。”

Inamura, K.: "Laminar distribution of pheromone receptive neurons in rat vomeronasal epihelium"J. Physiol. Lond.. 517. 731-739 (1999)

Inamura，K.：“大鼠犁鼻上皮信息素接受神经元的层状分布”J。

Inamura, K.: "Regionalization of Fos immunostaining in rat accessory olfactory bulb when the vomeronasal organ was exposed to urine"Eur. J. Neurosci.. 11, (in press). (1999)

Inamura, K.：“当犁鼻器官暴露于尿液时，大鼠副嗅球 Fos 免疫染色的区域化”Eur。