A new transduction mechanism of chemoreceptor cells and cloning of receptor protein

化学感受细胞新转导机制及受体蛋白克隆

• 批准号: 07457533
• 负责人: KURIHARA Kenzo
• 金额: $ 4.67万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457533/
• 关键词: phosphatidic acid; lipoproein; pheromone; urine; vomeronasal organ; cyclic AMP; IP3; rat; ファスファチジン酸; G蛋白質共役型受容体; 味細胞; 嗅細胞; 情報変換機構; グアニル酸シク-ラーゼ; クローニング; G-蛋白質共役型受容体; カエル

1) We examined the effects of lipoprotein composed of phosphatidic acid (PA) and beta-lactglobulin (LG) on bitterness of various chemicals by measuring nerve activities of frog glossopharyngeal nerve. PA-LG inhibited the bitterness of various drugs, but did not inhibit that of salts such as MgCl_2, CsCl and tetraethylammonium, suggesting that the receptor for bitter salts is different from other bitter compounds.2) We also examined whether a single olfactory cell responds only to specific odorants. The results obtained showed that a single olfactory cell respond to many different species of odorants, suggesting that a single olfactory cell has many different receptors.3) We prepared slice of rat vomeronasal epithelium and measured currents in response to cAMP and IP3. Injection of cAMP did not elicit current, but that of IP3 induced inward current, Female rat vomeronasal receptor cell responded to urine from male rat. This response was inhibited by inhibitors for production of IP3. These results suggest that IP3 may be a second messenger in reception of urinary pheromone in female rat vomeronasal receptor cell.

1）我们通过测量青蛙舌咽神经的神经活性，研究了由磷脂酸（PA）和β-乳球蛋白（LG）组成的脂蛋白对各种化学物质的苦味的影响。 PA-LG抑制了各种药物的苦味，但并未抑制MGCL_2，CSCL和四乙基铵等盐的苦味，这表明苦盐的受体与其他苦味化合物的受体不同。获得的结果表明，单个嗅觉细胞对许多不同种类的气味剂反应，这表明单个嗅觉细胞具有许多不同的受体。3）我们制备了大鼠呕吐的大鼠呕吐性上皮细胞的切片，并测量了对CAMP和IP3的响应。注射cAMP并没有引起电流，但是IP3诱导了内向电流，雌性大鼠呕吐受体细胞对雄性大鼠的尿液反应。这种反应被抑制剂抑制了IP3的产生。这些结果表明，IP3可能是女性大鼠呕吐受体细胞中尿素的第二信使。

项目成果

Y.Katsuragi: "Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste ; high affinity to frog tongue surface and hydrophobic model membranes" Biochem. Biophys. Acta. 1289. 322-328 (1996)

Y.Katsuragi：“选择性抑制苦味的含磷脂酸脂蛋白的特性；对青蛙舌头表面和疏水模型膜的高亲和力”Biochem。

M. Kashiwayanagi: "Existence of multiple receptors in single reurons : responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants" Brain. Res.738. 222-228 (1996)

M. Kashiwayanagi：“单个 reurons 中存在多个受体：单个牛蛙嗅觉神经元对许多 cAMP 依赖性和独立气味剂的反应”大脑。

M. Taniguchi: "Intracellular dialysis of cyclic nucleotide induces inward currents in the turtle vomeronasal receptor neurons" J. Neuersci. 16. 1239-1246 (1996)

M. Taniguchi：“环核苷酸的细胞内透析在乌龟犁鼻受体神经元中诱导内向电流”J. Neuersci。

Y.Katsuragi: ""Selective inhibition of bitter taste of various durgs by lipoprotein"" Pharm.Res.12. 658-662 (1995)

Y.Katsuragi：““脂蛋白对各种药物苦味的选择性抑制””Pharm.Res.12。

I.Matsuoka: "Identification of novel guanylyl cyclases from chemosensory tissues of rat and cattle" Biochim.Biophys.Res.Commun.216. 242-248 (1995)

I.Matsuoka：“从大鼠和牛的化学感应组织中鉴定新型鸟苷酸环化酶”Biochim.Biophys.Res.Commun.216。