A new transduction mechanism of chemoreceptor cells and cloning of receptor protein

化学感受细胞新转导机制及受体蛋白克隆

• 批准号: 07457533
• 负责人: KURIHARA Kenzo
• 金额: $ 4.67万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457533/
• 关键词: phosphatidic acid; lipoproein; pheromone; urine; vomeronasal organ; cyclic AMP; IP3; rat; ファスファチジン酸; G蛋白質共役型受容体; 味細胞; 嗅細胞; 情報変換機構; グアニル酸シク-ラーゼ; クローニング; G-蛋白質共役型受容体; カエル

1) We examined the effects of lipoprotein composed of phosphatidic acid (PA) and beta-lactglobulin (LG) on bitterness of various chemicals by measuring nerve activities of frog glossopharyngeal nerve. PA-LG inhibited the bitterness of various drugs, but did not inhibit that of salts such as MgCl_2, CsCl and tetraethylammonium, suggesting that the receptor for bitter salts is different from other bitter compounds.2) We also examined whether a single olfactory cell responds only to specific odorants. The results obtained showed that a single olfactory cell respond to many different species of odorants, suggesting that a single olfactory cell has many different receptors.3) We prepared slice of rat vomeronasal epithelium and measured currents in response to cAMP and IP3. Injection of cAMP did not elicit current, but that of IP3 induced inward current, Female rat vomeronasal receptor cell responded to urine from male rat. This response was inhibited by inhibitors for production of IP3. These results suggest that IP3 may be a second messenger in reception of urinary pheromone in female rat vomeronasal receptor cell.

1）我们通过测量青蛙舌咽神经的神经活动来检查由磷脂酸（PA）和β-乳球蛋白（LG）组成的脂蛋白对各种化学物质的苦味的影响。 PA-LG抑制多种药物的苦味，但不抑制MgCl_2、CsCl和四乙铵等盐类的苦味，这表明苦味盐的受体与其他苦味化合物不同。2)我们还检测了单个嗅觉细胞是否仅对特定气味有反应。获得的结果表明，单个嗅觉细胞对多种不同种类的气味有反应，这表明单个嗅觉细胞具有多种不同的受体。3)我们制备了大鼠犁鼻上皮切片并测量了对cAMP和IP3的响应电流。注射cAMP不引起电流，但注射IP3引起内向电流，雌性大鼠犁鼻受体细胞对雄性大鼠的尿液有反应。该反应被 IP3 产生抑制剂抑制。这些结果表明IP3可能是雌性大鼠犁鼻受体细胞接收尿信息素的第二信使。

项目成果

柏柳 誠: "嗅覚・味覚系におけるGTP結合蛋白質共役型受容体." 実験医学. 14. 68-70 (1996)

Makoto Kashiwayanagi：“嗅觉和味觉系统中的 GTP 结合蛋白偶联受体。”实验医学。14. 68-70 (1996)

Y.Katsuragi: "Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste ; high affinity to frog tongue surface and hydrophobic model membranes" Biochem. Biophys. Acta. 1289. 322-328 (1996)

Y.Katsuragi：“选择性抑制苦味的含磷脂酸脂蛋白的特性；对青蛙舌头表面和疏水模型膜的高亲和力”Biochem。

Y.Katsuragi: ""Selective inhibition of bitter taste of various durgs by lipoprotein"" Pharm.Res.12. 658-662 (1995)

Y.Katsuragi：““脂蛋白对各种药物苦味的选择性抑制””Pharm.Res.12。

M. Taniguchi: "Intracellular dialysis of cyclic nucleotide induces inward currents in the turtle vomeronasal receptor neurons" J. Neuersci. 16. 1239-1246 (1996)

M. Taniguchi：“环核苷酸的细胞内透析在乌龟犁鼻受体神经元中诱导内向电流”J. Neuersci。

M. Kashiwayanagi: "Existence of multiple receptors in single reurons : responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants" Brain. Res.738. 222-228 (1996)

M. Kashiwayanagi：“单个 reurons 中存在多个受体：单个牛蛙嗅觉神经元对许多 cAMP 依赖性和独立气味剂的反应”大脑。