A new transduction mechanism of chemoreceptor cells and cloning of receptor protein
化学感受细胞新转导机制及受体蛋白克隆
基本信息
- 批准号:07457533
- 负责人:
- 金额:$ 4.67万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) We examined the effects of lipoprotein composed of phosphatidic acid (PA) and beta-lactglobulin (LG) on bitterness of various chemicals by measuring nerve activities of frog glossopharyngeal nerve. PA-LG inhibited the bitterness of various drugs, but did not inhibit that of salts such as MgCl_2, CsCl and tetraethylammonium, suggesting that the receptor for bitter salts is different from other bitter compounds.2) We also examined whether a single olfactory cell responds only to specific odorants. The results obtained showed that a single olfactory cell respond to many different species of odorants, suggesting that a single olfactory cell has many different receptors.3) We prepared slice of rat vomeronasal epithelium and measured currents in response to cAMP and IP3. Injection of cAMP did not elicit current, but that of IP3 induced inward current, Female rat vomeronasal receptor cell responded to urine from male rat. This response was inhibited by inhibitors for production of IP3. These results suggest that IP3 may be a second messenger in reception of urinary pheromone in female rat vomeronasal receptor cell.
1)通过测量蛙舌咽神经的神经活动,考察了磷脂酸(PA)和β -乳酸球蛋白(LG)组成的脂蛋白对各种化学物质苦味的影响。PA-LG对多种药物的苦味均有抑制作用,但对MgCl_2、CsCl、四乙基铵等盐的苦味均无抑制作用,说明其对苦盐的感受器不同于其他苦味化合物。2)我们还研究了单个嗅觉细胞是否只对特定的气味做出反应。结果表明,一个嗅觉细胞对许多不同种类的气味有反应,这表明一个嗅觉细胞有许多不同的受体。3)制备大鼠犁鼻上皮切片,测定cAMP和IP3对电流的响应。注射cAMP不引起电流,但IP3引起内向电流,雌性大鼠犁鼻受体细胞对雄性大鼠尿液有反应。这种反应被产生IP3的抑制剂所抑制。这些结果提示IP3可能是雌性大鼠犁鼻受体细胞接收尿信息素的第二信使。
项目成果
期刊论文数量(52)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
柏柳 誠: "嗅覚・味覚系におけるGTP結合蛋白質共役型受容体." 実験医学. 14. 68-70 (1996)
Makoto Kashiwayanagi:“嗅觉和味觉系统中的 GTP 结合蛋白偶联受体。”实验医学。14. 68-70 (1996)
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- 影响因子:0
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Y.Katsuragi: "Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste ; high affinity to frog tongue surface and hydrophobic model membranes" Biochem. Biophys. Acta. 1289. 322-328 (1996)
Y.Katsuragi:“选择性抑制苦味的含磷脂酸脂蛋白的特性;对青蛙舌头表面和疏水模型膜的高亲和力”Biochem。
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- 影响因子:0
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Y.Katsuragi: ""Selective inhibition of bitter taste of various durgs by lipoprotein"" Pharm.Res.12. 658-662 (1995)
Y.Katsuragi:““脂蛋白对各种药物苦味的选择性抑制””Pharm.Res.12。
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M. Taniguchi: "Intracellular dialysis of cyclic nucleotide induces inward currents in the turtle vomeronasal receptor neurons" J. Neuersci. 16. 1239-1246 (1996)
M. Taniguchi:“环核苷酸的细胞内透析在乌龟犁鼻受体神经元中诱导内向电流”J. Neuersci。
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- 影响因子:0
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M. Kashiwayanagi: "Existence of multiple receptors in single reurons : responses of single bullfrog olfactory neurons to many cAMP-dependent and independent odorants" Brain. Res.738. 222-228 (1996)
M. Kashiwayanagi:“单个 reurons 中存在多个受体:单个牛蛙嗅觉神经元对许多 cAMP 依赖性和独立气味剂的反应”大脑。
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KURIHARA Kenzo其他文献
KURIHARA Kenzo的其他文献
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{{ truncateString('KURIHARA Kenzo', 18)}}的其他基金
Factory Automation Control System Designing Method Aiming High reliability and High Productivity of Software
以软件高可靠性和高生产率为目标的工厂自动化控制系统设计方法
- 批准号:
15560227 - 财政年份:2003
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
RARE KEY SUBSTANES OLFACTORY SENSE : IDETIFICATION OF HOME STREAM SUBSTANCES IN SALMON
稀有关键物质嗅觉:三文鱼中原生物质的识别
- 批准号:
12045257 - 财政年份:2000
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular discrimination and transduction in singe olfactory and vomeronasal receptor cells
单一嗅觉和犁鼻受体细胞的分子辨别和转导
- 批准号:
09480169 - 财政年份:1997
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of safety inhibitor which selectively masks receptor sites for bitter substances
开发选择性掩盖苦味物质受体位点的安全抑制剂
- 批准号:
09557185 - 财政年份:1997
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
New development of transduction mechanism in chemical senses
化学意义上转导机制的新进展
- 批准号:
07308049 - 财政年份:1995
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Masking of bitter taste and smell of drugs by protein-lipid complex prepared from materials for foods
用食品材料制备的蛋白质-脂质复合物掩盖药物的苦味和气味
- 批准号:
06557119 - 财政年份:1994
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Cloning of genes encoding putative taste receptor proteins
编码假定味觉受体蛋白的基因的克隆
- 批准号:
05454627 - 财政年份:1993
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Preparation of artificial membrane sensor which responds to various odorants
响应多种气味的人工膜传感器的制备
- 批准号:
01870096 - 财政年份:1989
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
Identification and Transduction Mechanisms in Chemoreceptor Cells
化学感受器细胞的识别和转导机制
- 批准号:
63480496 - 财政年份:1988
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Mechanisms of stimuli discrimination and transduction in gustatory and olfactory cells
味觉和嗅觉细胞的刺激辨别和转导机制
- 批准号:
60480457 - 财政年份:1985
- 资助金额:
$ 4.67万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)