Mechanisms of stimuli discrimination and transduction in gustatory and olfactory cells
味觉和嗅觉细胞的刺激辨别和转导机制
基本信息
- 批准号:60480457
- 负责人:
- 金额:$ 1.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1985
- 资助国家:日本
- 起止时间:1985 至 1986
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The discrimination mechanisms of chemical stimuli in taste and olfactory cells were studied using taste cells, neuroblastoma and liposomes. Among various taste stimuli, bitter substances were found to adsorb on lipid layers of the membranes, which leads to generation of the receptor potentials. "Taste blind" for phenylthiocarbamide in man was examined in terms of difference in lipid composition of the receptor membranes. As for the discrimination of odorants, various mechanisms have been proposed. Many investigators have believed that there exist specific receptor protein for each odorant, but question arises whether or not animals have respective specific receptor proteins for numerous odorants. We found that neuroblastoma cells and liposomes having certain lipid compositions are depolarized by various odorants similarly to olfactory cells. Changes in the lipid composition led to changes in specificity to various odorants. The present results suggested that odorant discrimination can be explained by posturating that lipid composition of receptor membrane of each olfactory cell is different from one cell to another.The mechanisms of changes in the membrane potentials in response to various taste stimuli and odorants were examined using taste organs, neuroblastoma, isolated olfactory cells and liposomes. Exchanges of ions in external medium with impermeable ions did not affect the changes of the membrane potentials in response to chemical stimuli. The membrane potential changes of lipid bilayer membranes in response to bitter substances and odorants occurred under the conditions that ion concentration at both sides of membranes is the same, suggesting that the membrane potential changes are induced by changes in the phase boundary potentials.
利用味觉细胞、神经母细胞瘤和脂质体研究了味觉和嗅觉细胞对化学刺激的识别机制。在各种味觉刺激中,苦味物质吸附在细胞膜的脂质层上,导致受体电位的产生。根据受体膜脂质组成的差异,对人苯硫胺的“味觉盲”进行了检查。关于气味的识别,人们提出了各种不同的机制。许多研究者认为每种气味都存在特定的受体蛋白,但问题是动物是否对多种气味都有各自的特异性受体蛋白。我们发现神经母细胞瘤细胞和具有一定脂质成分的脂质体被各种气味剂去极化,类似于嗅觉细胞。脂质组成的变化导致对各种气味的特异性变化。目前的研究结果表明,气味识别可以通过每个细胞的受体膜的脂质组成不同来解释。利用味觉器官、神经母细胞瘤、分离的嗅觉细胞和脂质体研究了不同味觉刺激和气味刺激下膜电位变化的机制。外介质中离子与不渗透离子的交换不影响膜电位在化学刺激下的变化。脂质双层膜对苦味物质和气味物质的膜电位变化发生在膜两侧离子浓度相同的条件下,表明膜电位变化是由相边界电位的变化引起的。
项目成果
期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nomura, T.; Kurihara, K.: "Effects of changed lipid composition on responses of liposomes to various odorants: A possible mechanism of odor discrimination." Submitted to Biochemistry. (1987)
野村,T.;
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Yoshii, K.; Kiyomoto, Y.; Kurihara, K.: "Taste receptor mechanism of salts in frog and rat." Comp. Biochem. Physiol.85A. 501-507 (1986)
吉井,K.;
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Nomura, T.; Kurihara, K.: "Liposomes as model for olfactory cells: Changes membranes potential in response to various odorants." Submitted to Biochemistry. (1987)
野村,T.;
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KURIHARA Kenzo其他文献
KURIHARA Kenzo的其他文献
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{{ truncateString('KURIHARA Kenzo', 18)}}的其他基金
Factory Automation Control System Designing Method Aiming High reliability and High Productivity of Software
以软件高可靠性和高生产率为目标的工厂自动化控制系统设计方法
- 批准号:
15560227 - 财政年份:2003
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
RARE KEY SUBSTANES OLFACTORY SENSE : IDETIFICATION OF HOME STREAM SUBSTANCES IN SALMON
稀有关键物质嗅觉:三文鱼中原生物质的识别
- 批准号:
12045257 - 财政年份:2000
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular discrimination and transduction in singe olfactory and vomeronasal receptor cells
单一嗅觉和犁鼻受体细胞的分子辨别和转导
- 批准号:
09480169 - 财政年份:1997
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of safety inhibitor which selectively masks receptor sites for bitter substances
开发选择性掩盖苦味物质受体位点的安全抑制剂
- 批准号:
09557185 - 财政年份:1997
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
New development of transduction mechanism in chemical senses
化学意义上转导机制的新进展
- 批准号:
07308049 - 财政年份:1995
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
A new transduction mechanism of chemoreceptor cells and cloning of receptor protein
化学感受细胞新转导机制及受体蛋白克隆
- 批准号:
07457533 - 财政年份:1995
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Masking of bitter taste and smell of drugs by protein-lipid complex prepared from materials for foods
用食品材料制备的蛋白质-脂质复合物掩盖药物的苦味和气味
- 批准号:
06557119 - 财政年份:1994
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Cloning of genes encoding putative taste receptor proteins
编码假定味觉受体蛋白的基因的克隆
- 批准号:
05454627 - 财政年份:1993
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Preparation of artificial membrane sensor which responds to various odorants
响应多种气味的人工膜传感器的制备
- 批准号:
01870096 - 财政年份:1989
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
Identification and Transduction Mechanisms in Chemoreceptor Cells
化学感受器细胞的识别和转导机制
- 批准号:
63480496 - 财政年份:1988
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
Evaluation of odourant chemicals on fish olfactory cell cultures and fish cell lines
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Glycoconjugates in Olfactory Cell-Cell Interactions
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8116580 - 财政年份:1992
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3217714 - 财政年份:1992
- 资助金额:
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7906815 - 财政年份:1992
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