Cloning of genes encoding putative taste receptor proteins

编码假定味觉受体蛋白的基因的克隆

• 批准号: 05454627
• 负责人: KURIHARA Kenzo
• 金额: $ 4.48万
• 依托单位: HOKKAIDO UNlVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454627/
• 关键词: guanlyly cyclase; G-protein coupled receptor; taste; cloning; putative taste receptor; rat; cattle; olfactory receptor; 味覚受容体; GTP結合蛋白質; cDNA; ゼブラフィッシュ; アミノ酸; リポソーム; 苦味物質; 甘味物質; ウシ味覚器; ペプチド受容体; cAMP; IP3

Using reverse transcription-polymerase chain reaction and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Type A clones were expressed in renal as well as in taste tissue.A number of studies have shown that cGMP may play some roles in taste transduction. For example, guanylyl cyclase is reported to be localized at microvilli of taste cells. To identify the structure of guanylyl cyclase in taste tissue, cDNA fragments encoding guanylyl cyclase catalytic domain wre amplified from rat and bovine tongue epithelium using degenerate oligonuclotide primers and reverse transcription-polymerase chain reaction. Three novel clones, two membrane type guanylyl cyclases (RAT GC-1, BOV GC-3) and one soluble type guanylyl cyclase (RAT GC-2). RAT GC-1 was distributed over various rat tissues in addition to taste tissue. BOV GC-3 was similar to but distinct from recent cloned olfactory specific guanlylyl cyclase. Rat GC-2 was identified as rat homologue of alpha2 subunit of the soluble guanylyl cyclase.

使用逆转录聚合酶链反应和退化的寡核苷酸引物，我们扩大了来自牛味组织的两个不同亚科G蛋白偶联受体（GCR）的新成员。由多个cDNA克隆组成的A型受体克隆与假定的嗅觉受体亚科具有显着相似之处，而单个B型克隆具有与肽受体亚家族的显着相似性。 A型克隆在肾脏和味道组织中均表达。一项研究表明，CGMP可能在味觉转导中起一些作用。例如，据报道，鸟叶叶尼环酶位于味道细胞的微绒毛上。为了鉴定味道组织中的鸟叶基环化酶的结构，使用退化的寡核核肽引物和逆转录 - 促 - 促酶链反应从大鼠和牛舌上皮放大了编码鸟叶环酶催化结构域的cDNA片段。三个新颖的克隆，两个膜型鸟叶酸环化酶（大鼠GC-1，BOV GC-3）和一个可溶性类型的鸟叶酸环酶（大鼠GC-2）。除了味道组织外，大鼠GC-1在各种大鼠组织上分布。 BOV GC-3与最近克隆的嗅觉特异性鸟氰基环化酶相似，但与众不同。大鼠GC-2被鉴定为可溶性鸟叶基环酶α2亚基的大鼠同源物。

项目成果

Yoshihisa Katsuragi and Kenzo Kurihara: "Specific inhibitor for bitter taste." Nature. 365. 213-214 (1993)

Yoshihisa Katsuragi 和 Kenzo Kurihara：“苦味的特殊抑制剂。”

I.Matsuoka, T.Mori, T.Sato, M.Sakai and K.Kurihara: "Identification of novel guanylyl cyclases from chemosensory tissues of rat and cattle" Biochem.Biophy.Res.Commun.216. 242-248 (1995)

I.Matsuoka、T.Mori、T.Sato、M.Sakai 和 K.Kurihara：“从大鼠和牛的化学感应组织中鉴定新型鸟苷酸环化酶”Biochem.Biophy.Res.Commun.216。

松岡 一郎: "G蛋白質共役型受容体スーパーファミリー" 生体の科学. 46. 545-549 (1995)

松冈一郎：“G 蛋白偶联受体超家族”《生物科学》46. 545-549 (1995)。

M.Nakamura, K.Tsujii, Y.Katsuragi, K Kurihara and J.Sunamoto: Biochem.Biophys.Res.Commun.201. 415-422 (1994)

M.Nakamura、K.Tsujii、Y.Katsuragi、K Kurihara 和 J.Sunamoto：Biochem.Biophys.Res.Commun.201。

M.Kashiwayanagi: "Discrimination of odorants in the non-olfactory system:analysis of responses of the frog gustatory system to odorants by multidimensional scaling" Comparative Biochemistry and Physiology. 108A. 479-484 (1994)

M.Kashiwayanagi：“非嗅觉系统中气味的辨别：通过多维尺度分析青蛙味觉系统对气味的反应”比较生物化学和生理学。