Cloning of genes encoding putative taste receptor proteins

编码假定味觉受体蛋白的基因的克隆

• 批准号: 05454627
• 负责人: KURIHARA Kenzo
• 金额: $ 4.48万
• 依托单位: HOKKAIDO UNlVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454627/
• 关键词: guanlyly cyclase; G-protein coupled receptor; taste; cloning; putative taste receptor; rat; cattle; olfactory receptor; 味覚受容体; GTP結合蛋白質; cDNA; ゼブラフィッシュ; アミノ酸; リポソーム; 苦味物質; 甘味物質; ウシ味覚器; ペプチド受容体; cAMP; IP3

Using reverse transcription-polymerase chain reaction and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Type A clones were expressed in renal as well as in taste tissue.A number of studies have shown that cGMP may play some roles in taste transduction. For example, guanylyl cyclase is reported to be localized at microvilli of taste cells. To identify the structure of guanylyl cyclase in taste tissue, cDNA fragments encoding guanylyl cyclase catalytic domain wre amplified from rat and bovine tongue epithelium using degenerate oligonuclotide primers and reverse transcription-polymerase chain reaction. Three novel clones, two membrane type guanylyl cyclases (RAT GC-1, BOV GC-3) and one soluble type guanylyl cyclase (RAT GC-2). RAT GC-1 was distributed over various rat tissues in addition to taste tissue. BOV GC-3 was similar to but distinct from recent cloned olfactory specific guanlylyl cyclase. Rat GC-2 was identified as rat homologue of alpha2 subunit of the soluble guanylyl cyclase.

使用逆转录聚合酶链反应和简并寡核苷酸引物，我们从牛味觉组织中扩增了 G 蛋白偶联受体 (GCR) 超家族两个不同亚家族的新成员。由多个cDNA克隆组成的A型受体克隆与假定的嗅觉受体亚家族具有显着的相似性，而单个B型受体克隆与肽受体亚家族具有显着的相似性。 A型克隆在肾脏和味觉组织中都有表达。大量研究表明cGMP可能在味觉转导中发挥一定作用。例如，据报道，鸟苷酸环化酶位于味觉细胞的微绒毛处。为了鉴定味觉组织中鸟苷酸环化酶的结构，使用简并寡核苷酸引物和逆转录聚合酶链式反应从大鼠和牛舌上皮中扩增编码鸟苷酸环化酶催化结构域的cDNA片段。三个新克隆，两种膜型鸟苷酸环化酶（RAT GC-1、BOV GC-3）和一种可溶型鸟苷酸环化酶（RAT GC-2）。除了味觉组织外，RAT GC-1 还分布在各种大鼠组织中。 BOV GC-3 与最近克隆的嗅觉特异性鸟苷酸环化酶相似但不同。大鼠GC-2被鉴定为可溶性鸟苷酸环化酶α2亚基的大鼠同源物。

项目成果

松岡 一郎: "G蛋白質共役型受容体スーパーファミリー" 生体の科学. 46. 545-549 (1995)

松冈一郎：“G 蛋白偶联受体超家族”《生物科学》46. 545-549 (1995)。

I.Matsuoka, T.Mori, T.Sato, M.Sakai and K.Kurihara: "Identification of novel guanylyl cyclases from chemosensory tissues of rat and cattle" Biochem.Biophy.Res.Commun.216. 242-248 (1995)

I.Matsuoka、T.Mori、T.Sato、M.Sakai 和 K.Kurihara：“从大鼠和牛的化学感应组织中鉴定新型鸟苷酸环化酶”Biochem.Biophy.Res.Commun.216。

Yoshihisa Katsuragi and Kenzo Kurihara: "Specific inhibitor for bitter taste." Nature. 365. 213-214 (1993)

Yoshihisa Katsuragi 和 Kenzo Kurihara：“苦味的特殊抑制剂。”

M.Nakamura, K.Tsujii, Y.Katsuragi, K Kurihara and J.Sunamoto: Biochem.Biophys.Res.Commun.201. 415-422 (1994)

M.Nakamura、K.Tsujii、Y.Katsuragi、K Kurihara 和 J.Sunamoto：Biochem.Biophys.Res.Commun.201。

M.Kashiwayanagi: "Discrimination of odorants in the non-olfactory system:analysis of responses of the frog gustatory system to odorants by multidimensional scaling" Comparative Biochemistry and Physiology. 108A. 479-484 (1994)

M.Kashiwayanagi：“非嗅觉系统中气味的辨别：通过多维尺度分析青蛙味觉系统对气味的反应”比较生物化学和生理学。