Cloning of genes encoding putative taste receptor proteins

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Using reverse transcription-polymerase chain reaction and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Type A clones were expressed in renal as well as in taste tissue.A number of studies have shown that cGMP may play some roles in taste transduction. For example, guanylyl cyclase is reported to be localized at microvilli of taste cells. To identify the structure of guanylyl cyclase in taste tissue, cDNA fragments encoding guanylyl cyclase catalytic domain wre amplified from rat and bovine tongue epithelium using degenerate oligonuclotide primers and reverse transcription-polymerase chain reaction. Three novel clones, two membrane type guanylyl cyclases (RAT GC-1, BOV GC-3) and one soluble type guanylyl cyclase (RAT GC-2). RAT GC-1 was distributed over various rat tissues in addition to taste tissue. BOV GC-3 was similar to but distinct from recent cloned olfactory specific guanlylyl cyclase. Rat GC-2 was identified as rat homologue of alpha2 subunit of the soluble guanylyl cyclase.