New development of transduction mechanism in chemical senses

化学意义上转导机制的新进展

• 批准号: 07308049
• 负责人: KURIHARA Kenzo
• 金额: $ 5.89万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07308049/
• 关键词: taste; Olfaction; saccharine; transduction; turtle; mouse; phospholipids; bitterness; パッチクランプ法; カエル; ハエ

Various results on olfactory and gustatory receptor mechanisms were obtained.(1) It was found that there is a transduction pathway independent of cAMP and IP3 in olfactory cell. This pathway was independent of Ca, NO and cGMP.It was also found that membrane fluidity changes of turtle olfactory cell affect ability to discriminate odorants, suggesting that lipid layr of olfactory cell membranes in involved in oder reception.(2) It was found that application of low concentration of saccharine to mouse taste cells increased cAMP level and that of high concentration of saccharine increased IP3. The suger response in fly taste cell was suppressed by inhibitors for production of IP3, suggesting that IP3 is involved in sugar reception in fly taste cell. It was also found that bitterness of various substances is suppressed by acidic phospholipids such as phosphatidic acid, phosphatidylinositol and phosphatidylserine. Among these lipids, phosphatidic acid was most effective to suppress bitterness.

嗅觉和味觉受体机制的各种结果。(1)结果发现，在嗅细胞中存在一条不依赖cAMP和IP3的信号转导通路。该通路不依赖于Ca、NO和cGMP，同时还发现嗅细胞膜流动性的变化影响嗅细胞对气味的辨别能力，提示嗅细胞膜脂质层参与了气味的接收。(2)结果发现，低浓度糖精对小鼠味觉细胞的应用增加了cAMP水平，而高浓度糖精则增加了IP 3。果蝇味觉细胞的糖反应被IP3产生抑制剂抑制，提示IP3参与了果蝇味觉细胞的糖接收。还发现各种物质的苦味被酸性磷脂如磷脂酸、磷脂酰肌醇和磷脂酰丝氨酸抑制。在这些脂质中，磷脂酸是最有效的抑制苦味。

项目成果

M.Taniguchi: "Difference in behavior between responses to forskolin and general odorants in turtle vomeronasal organ." Chem.Senses. 21. 763-777 (1996)

M.Taniguchi：“乌龟犁鼻器对毛喉素和一般气味剂的反应之间的行为差​​异。”

M.Koganezawa: "The effect of G protein modulators on the labellar taste receptor cells of the fleshfly (Boettcherisca Pergrina)" J.Insect Physiol.43. 225-233 (1997)

M.Koganezawa：“G 蛋白调节剂对肉蝇 (Boettcherisca Pergrina) 标签味觉受体细胞的影响”J.Insect Physiol.43。

M. Taniguch: "Intracellular dialysis of cyclic nucleotides induces inward currents in turtle vomeronasal receptor neurons." J. Nearosci. 16・3. 1239-1246 (1996)

M. Taniguch：“环核苷酸的细胞内透析诱导乌龟犁鼻受体神经元的内向电流。”J. Nearosci 16·3（1996）。

Y.Katsuragi: "Selective inhibition of bitter taste of various drugs by lipoprotein" Pharm.Res.12. 658-662 (1995)

Y.Katsuragi：“脂蛋白对各种药物苦味的选择性抑制”Pharm.Res.12。

H.Nagao: "Effects of adenylyl cyclase-linked neuropeptides on the expression of CNTF-mRNA in cultured glial cells from rat olfactory bulb and cerebral cortex" FEBS Lett.362. 75-79 (1995)

H.Nagao：“腺苷酸环化酶连接的神经肽对大鼠嗅球和大脑皮层培养的神经胶质细胞中 CNTF-mRNA 表达的影响”FEBS Lett.362。