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Studies on the mechanism of antibody production of carp in a myxosporean infection

鲤鱼粘孢子虫感染产生抗体机制的研究

基本信息

批准号：
04660197
负责人：
OGAWA Kazuo
金额：
$ 1.28万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660197/
关键词：
Cyprinus carpio myxosporeans Myxobolus artus antibody production vaccine 寄生虫 Cyprinus carpio 宿主反応

项目摘要

Myxobolus artus formed pseudocysts in the skeletal muscle of carp. Some pseudocysts showed abnormal growth, imperfect encapsulation of the parasite by the host tissue, resulting in rupture of pseudocysts before completion of sporogenesis. After spore formation was complete or atypical pseudocysts ruptured, host reaction was initiated. Spores were phagocytosed and either transported to macrophage centers in the visceral organs or discharged from the host through the skin and intestine.The antibody which reacted with sonicated spores was detected in some naturally infected carp, while some other fish had no detectable sign of infection though they had the antibody. The antibody was produced in carp injected either with developing stages of the parasite or sonicated spores with bovine serum albumin, but not in carp injected either with intact or sonicated spores. The results of the injection experiments suggest that 1) developing stages have antigenicity to carp and 2) spores have lost it ; sonicated spores are haptens, with which the antibody can react. In an indirect fluorescent antibody technique, sera positive for the antigen reacted with developing stages of the parasite, but not with the spore. It is plausible that the antibody was produced when young pseudocysts, which showed abnormal growth ruptured during development, were exposed to the host immune system. In vaccination trials, carp injected with sonicated spores did not show any immutity agaist M.artus infection.

艺术碘泡虫在鲤鱼骨骼肌中形成假包囊。有些假孢囊表现出异常生长，宿主组织对寄生虫的包裹不完善，导致假孢囊在孢子形成完成之前破裂。孢子形成完成或非典型假孢囊破裂后，宿主反应开始。在自然感染的鲤鱼中检测到与声振孢子发生反应的抗体，而在其它鱼中检测到与声振孢子发生反应的抗体，但没有检测到感染的迹象。在鲤鱼注射无论是与发展阶段的寄生虫或超声处理的孢子与牛血清白蛋白，但不是在鲤鱼注射无论是完整的或超声处理的孢子。注射实验结果表明：1）发育阶段对鲤鱼具有抗原性，2）孢子已失去抗原性;超声处理的孢子是半抗原，抗体可与之反应。在间接荧光抗体技术中，抗原阳性血清与寄生虫的发育阶段反应，但不与孢子反应。这种抗体可能是在发育过程中出现异常生长破裂的年轻假囊肿暴露于宿主免疫系统时产生的。在接种试验中，用超声处理的孢子注射鲤鱼没有显示出任何抵抗弓丝支原体感染的免疫力。