Molecular and Morphological Studies on Mechanism for Maternal Inheritance of Chloroplast Genomes in Chlamydomonas reinhardtii.

在莱茵衣藻（Chlamydomonas reinhardtii）中，雌雄配子融合后，雄性配子起源的叶绿体DNA在合子中优先消失，但这种优先破坏的机制尚不清楚。我们之前已经证明，当渗透配子与衣藻核酸酶C在Ca^<2+>存在下孵育时，来自雄性起源的叶绿体DNA被优先破坏，而雌性起源的叶绿体DNA显然完好无损。然后，在本工作中，我们研究了雌性和雄性配子的分子和形态差异。制备了针对幼小受精卵细胞表面的单克隆抗体，以澄清它们之间的区别。制备了保持完整形态的叶绿体类核，以研究它们的差异是否由于与细胞器dna结合的dna结合蛋白的差异。DNA结合蛋白在sds -凝胶中可被检测到。从特异性和敏感性方面讨论了DAPI代替EtBr的有效性。

In Chlamydomonas reinhardtii, the chloroplast DNA of male gamete origin preferentially disappears in the zygote after female and male gametes fuse sexully with each other, but the mechanism underlying this preferential dest-ruction remains to be established.We have previously shown that when the permeabilized gametes were incuba-ted with Chlamydomonas nuclease C in the presence of Ca^<2+>, chloroplast DNA from the male origin was preferentially destroyed, leaving female one apparently intact. Then, in the present work, we studied on the molecular and morpholo-gical difference between female and male gametes.1. Monoclonal antibody directed to cell surface of young zygotes was pre-pared to clarify the difference between them.2. Chloroplast nucleoids retaining the intact morphology were prepared to investigate whether their difference was due to that of DNA-binding proteins bound to organella DNA.3. The DNA-binding proteins could be detectable in SDS-gel containing DNA after their renaturations. Usefulness of DAPI instead of EtBr was discussed in connection of specificity and sensitivity.