High water temperature treatment for the control of Gluger pleceglassi infection of cultuned ayu, Plecoglossus altivelis

高水温处理控制养殖香鱼Plecoglossus altivelis的Gluger pleceglassi感染

基本信息

批准号：
06660228
负责人：
OGAWA Kazuo
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

A high water treatment method has been established against Glugea plecoglossi-infected ayu. Plecoglossus altivelis, using artificially infected fish through oral administration of spores.Glugea cysts completely disappeared after fish were maintained at 29ﾟC for 5 days 11-26 days after the spore administration, followed by the same treatment 7 days after the first treatment. A comparable result was obtained when naturally infected fish were similarly treated.Ayu immuno-globlin was purified and an ELISA system has been established to detect antibodies against whole spores. The antibody was detected as early as 10 days after oral administration of spores to ayu. Since spores taken from the intestinal surface of ayu were confirmed by the Uvitex 2B staining, it was thought that the antibody was produced against the injested spores.Using ayu with a 30% prevalence of infection at the start, challenge tests were conducted through oral administration of live spores to ayu after treatment with high temperature water. As a result, no firm evidence was obtained that the fish had acquired immunity against the microsporidian infection through the treatment, though elevation of the antibody levels were recognized in some fish. The fact that control fish (treated but not challenged) had a significantly lower prevalence of infection and smaller number of cysts than challeged fish confirmed the efficacy of the treatment method.

已经针对Glugea Plecoglossi感染的Ayu建立了高水处理方法。 plecoglossus altivelis，通过口服孢子进行人工感染的鱼类。glugea囊肿在腐烂给药后的29°C保持在29°C后11-26天将鱼类保持在29°C后完全消失，然后在第一次治疗后7天进行相同的治疗。当对自然感染的鱼类似处理时，获得了可比的结果。纯化了免疫绿蛋白，并建立了ELISA系统以检测针对整个孢子的抗体。口服孢子到AYU后10天，最早检测到该抗体。由于Uvitex 2B染色证实了从AYU的肠表面取走的孢子，因此人们认为该抗体是针对喷发孢子的。开始在开始时感染30％的AYU，挑战测试是通过对高温水治疗后的Ayu进行的，通过对Ayu进行口服给AYU进行挑战测试。结果，尚无牢固的证据表明，尽管在某些鱼类中识别出抗体水平的升高，但通过治疗获得了对微孢子虫感染的免疫力。对照鱼（治疗但没有挑战）的事实显着降低了感染的患病率，而囊肿数量较少，而囊肿数量较少，证实了治疗方法的效率。