High water temperature treatment for the control of Gluger pleceglassi infection of cultuned ayu, Plecoglossus altivelis

高水温处理控制养殖香鱼Plecoglossus altivelis的Gluger pleceglassi感染

• 批准号: 06660228
• 负责人: OGAWA Kazuo
• 金额: $ 1.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660228/
• 关键词: parasite; Microsporidia; Gluger plecoglessi; Plecoglossus altivelis; treatment; glugeosis; ELISA; immunology; Glugea Plecoglossi; Placoglossus altivelis

A high water treatment method has been established against Glugea plecoglossi-infected ayu. Plecoglossus altivelis, using artificially infected fish through oral administration of spores.Glugea cysts completely disappeared after fish were maintained at 29ﾟC for 5 days 11-26 days after the spore administration, followed by the same treatment 7 days after the first treatment. A comparable result was obtained when naturally infected fish were similarly treated.Ayu immuno-globlin was purified and an ELISA system has been established to detect antibodies against whole spores. The antibody was detected as early as 10 days after oral administration of spores to ayu. Since spores taken from the intestinal surface of ayu were confirmed by the Uvitex 2B staining, it was thought that the antibody was produced against the injested spores.Using ayu with a 30% prevalence of infection at the start, challenge tests were conducted through oral administration of live spores to ayu after treatment with high temperature water. As a result, no firm evidence was obtained that the fish had acquired immunity against the microsporidian infection through the treatment, though elevation of the antibody levels were recognized in some fish. The fact that control fish (treated but not challenged) had a significantly lower prevalence of infection and smaller number of cysts than challeged fish confirmed the efficacy of the treatment method.

建立了一种防治香鱼多舌胶壳虫病的高效水处理方法。用人工感染的香鱼经口投喂孢子，在投喂孢子后11-26天，在29 ℃下保持5天，然后在第一次投喂孢子后7天再进行同样的处理，Glugea包囊完全消失。纯化了Ayu免疫球蛋白，建立了检测抗完整孢子抗体的ELISA体系。在香鱼口服孢子后10天就检测到抗体。由于从香鱼肠道表面采集的孢子经Uvitex 2B染色证实，因此认为抗体是针对感染的孢子产生的。使用开始时感染率为30%的香鱼，在用高温水处理后，通过口服活孢子对香鱼进行攻击试验。结果，尽管在一些鱼中发现抗体水平升高，但没有获得确凿的证据表明鱼通过治疗获得了针对微孢子虫感染的免疫力。事实上，对照鱼（治疗，但没有挑战）有一个显着较低的感染率和更少的数量的囊肿比challaged鱼证实了治疗方法的疗效。