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High water temperature treatment for the control of Gluger pleceglassi infection of cultuned ayu, Plecoglossus altivelis

高水温处理控制养殖香鱼Plecoglossus altivelis的Gluger pleceglassi感染

基本信息

批准号：
06660228
负责人：
OGAWA Kazuo
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

A high water treatment method has been established against Glugea plecoglossi-infected ayu. Plecoglossus altivelis, using artificially infected fish through oral administration of spores.Glugea cysts completely disappeared after fish were maintained at 29ﾟC for 5 days 11-26 days after the spore administration, followed by the same treatment 7 days after the first treatment. A comparable result was obtained when naturally infected fish were similarly treated.Ayu immuno-globlin was purified and an ELISA system has been established to detect antibodies against whole spores. The antibody was detected as early as 10 days after oral administration of spores to ayu. Since spores taken from the intestinal surface of ayu were confirmed by the Uvitex 2B staining, it was thought that the antibody was produced against the injested spores.Using ayu with a 30% prevalence of infection at the start, challenge tests were conducted through oral administration of live spores to ayu after treatment with high temperature water. As a result, no firm evidence was obtained that the fish had acquired immunity against the microsporidian infection through the treatment, though elevation of the antibody levels were recognized in some fish. The fact that control fish (treated but not challenged) had a significantly lower prevalence of infection and smaller number of cysts than challeged fish confirmed the efficacy of the treatment method.

已经建立了针对受Glugea plecoglossi感染的香鱼的高效水处理方法。 Plecoglossus altivelis，使用通过口服孢子的方式人工感染的鱼。在给予孢子后的11-26天，将鱼在29℃下维持5天后，糖囊完全消失，在第一次治疗后7天进行相同的治疗。当自然感染的鱼进行类似处理时，获得了类似的结果。纯化了香鱼免疫球蛋白，并建立了 ELISA 系统来检测针对全孢子的抗体。香鱼口服孢子后10天就可以检测到抗体。由于Uvitex 2B染色确认了从香鱼肠道表面取出的孢子，因此认为针对注射的孢子产生了抗体。使用最初感染率30%的香鱼，通过高温水处理后将活孢子口服给香鱼进行攻击试验。结果，虽然在一些鱼中发现抗体水平升高，但没有获得确凿的证据表明鱼通过治疗获得了针对微孢子虫感染的免疫力。与攻击鱼相比，对照鱼（经过处理但未受到攻击）的感染率明显较低，包囊数量也较少，这一事实证实了治疗方法的有效性。