Evaluation of Environmental Amenity for Animals based on Variability of Parameter of Physiological Function.

基于生理功能参数变异性的动物环境适宜性评价。

基本信息

批准号：
06650448
负责人：
TAZAWA Hiroshi
金额：
$ 0.83万
依托单位：
Muroran Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06650448/
关键词：
Avian egg Instantaneous heart rate of embryo BCG measurement by means of electromagnetic induction coil Transient bradycardia Heart rate variability Development of autonomic nervous functions Exposure to low or high environmental temperature /

项目摘要

(1) As an experimental animal model, chick embryos were investigated for their physiological functions and growth to evaluate sdverse effects of pre-incubation egg storage and failure to turn eggs during the early incubation period.(2) Two-dimensional cardiogenic ballistic trajectories were elucidated by depicting them on a computer monitor screen from ballistic signals measured simultaneously at two sites on the eggshell with laser displacement meters.(3) Another simple method to measure the cardiogenic ballistic movements (BCG) of eggs was developed by taking advantage of an electromagnetic induction coil (solenoid) and small permanent magnet as a sensor. Numerical model analysis was made to design a measuring system and the BCG was successfully measured from late chick embryos.(4) A noninvasive system of instantaneous heart rate (IHR) was developed using acoustocardiogram and determined the IHR and its variability of individual chick embryos during the last half of incubation. Characteristic, transient bradycardia was found to occur on days 14-15 and the embryonic cardiac rhythm became more arrhythmic with further development. Experiments using intravenous administration technique of autonomic drugs showed no relationship between functional development of autonomic nerves and unique embryonic arrhythmia and heart rate variability (HRV). These new evidences prompt us further study to elucidate the HRV.(5) It was hypothesized that the optimal incubation temperature (38ﾟC) in air provides developing embryos with the most favorable amenity and the HRV is at maximum in the favorable amenity. The IHR and HRV of developing embryos were determined at 38ﾟC in air and during acute exposure to altered environments (low or high temperature, and hypoxic or hyperoxic environment). Preliminary results indicate that the HRV decreases in an altered environment and detailed experiments are in progress.

（1）作为一个实验性动物模型，研究了雏鸡的身体功能和增长，以评估储存前卵的稳定作用，并在孵化早期的早期孵化期内未能转动卵。通过利用电磁诱导线圈（电磁阀）和小型永久磁铁作为传感器，开发了一种测量卵心脏弹道运动（BCG）的简单方法。进行了数值模型分析以设计测量系统，并通过使用Aquastocarpiongram图开发了一种无创的瞬时心率（IHR）侵入性系统，并确定了IHR及其在孵育后半段的单个雏鸡胚胎的变异性。发现在第14-15天发生特征性的短暂性心动过缓，并且随着进一步的发展，胚胎心律变得更加心律不齐。使用静脉内给药技术的实验表明，自主神经的功能发展与独特的胚胎心律失常与心率变异性（HRV）之间没有关系。这些新的证据促使我们进一步研究以阐明HRV。（5）假设空气中的最佳孵育温度（38°C）为发育中的胚胎提供了最有利的便利性，而HRV则最大程度地达到了有利的便利性。在空气中和急性暴露于改变的环境（低温或高温以及低氧或高氧化环境）时，在38 c c下确定发育胚胎的IHR和HRV。初步结果表明，HRV在改变的环境中减少，并且正在进行详细的实验。