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Evaluation of Environmental Amenity for Animals based on Variability of Parameter of Physiological Function.

基于生理功能参数变异性的动物环境适宜性评价。

基本信息

批准号：
06650448
负责人：
TAZAWA Hiroshi
金额：
$ 0.83万
依托单位：
Muroran Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06650448/
关键词：
Avian egg Instantaneous heart rate of embryo BCG measurement by means of electromagnetic induction coil Transient bradycardia Heart rate variability Development of autonomic nervous functions Exposure to low or high environmental temperature /

项目摘要

(1) As an experimental animal model, chick embryos were investigated for their physiological functions and growth to evaluate sdverse effects of pre-incubation egg storage and failure to turn eggs during the early incubation period.(2) Two-dimensional cardiogenic ballistic trajectories were elucidated by depicting them on a computer monitor screen from ballistic signals measured simultaneously at two sites on the eggshell with laser displacement meters.(3) Another simple method to measure the cardiogenic ballistic movements (BCG) of eggs was developed by taking advantage of an electromagnetic induction coil (solenoid) and small permanent magnet as a sensor. Numerical model analysis was made to design a measuring system and the BCG was successfully measured from late chick embryos.(4) A noninvasive system of instantaneous heart rate (IHR) was developed using acoustocardiogram and determined the IHR and its variability of individual chick embryos during the last half of incubation. Characteristic, transient bradycardia was found to occur on days 14-15 and the embryonic cardiac rhythm became more arrhythmic with further development. Experiments using intravenous administration technique of autonomic drugs showed no relationship between functional development of autonomic nerves and unique embryonic arrhythmia and heart rate variability (HRV). These new evidences prompt us further study to elucidate the HRV.(5) It was hypothesized that the optimal incubation temperature (38ﾟC) in air provides developing embryos with the most favorable amenity and the HRV is at maximum in the favorable amenity. The IHR and HRV of developing embryos were determined at 38ﾟC in air and during acute exposure to altered environments (low or high temperature, and hypoxic or hyperoxic environment). Preliminary results indicate that the HRV decreases in an altered environment and detailed experiments are in progress.

(1)以鸡胚为实验动物模型，研究了鸡胚的生理功能和生长发育，以评价孵化前鸡蛋储存和早期孵化失败的不利影响。(2)利用激光位移仪同时测量蛋壳上两个部位的弹道信号，在计算机屏幕上描绘二维心源性弹道轨迹。(3)利用电磁感应线圈(螺线管)和小永磁体作为传感器，建立了另一种测量鸡蛋心源性弹道运动(BCG)的简单方法。建立了一套基于心动图的无创瞬时心率测量系统，并对孵化后半个阶段的单个鸡胚的瞬时心率及其变异性进行了测定。特征是，短暂性心动过缓发生在第14-15天，随着发育的深入，胚胎心律失常变得更加严重。静脉注射自主神经药物的实验表明，自主神经的功能发育与独特的胚胎心律失常和心率变异性(HRV)无关。这些新的证据促使我们进一步研究以阐明心率变异性。(5)假设空气中的最适孵化温度(38ﾟC)为发育胚胎提供最有利的舒适性，并且在良好的舒适性中心率变异性最大。在38ﾟC空气中和急性暴露于改变的环境(低温或高温、低氧或高氧环境)的条件下，测定了发育胚胎的心率和心率变异性。初步结果表明，心率变异性在改变的环境中降低，详细的实验正在进行中。