A closed-circuit continuous measurement system for metabolic rate and physioengineering study on thermoregulation of chick embryos.

鸡胚代谢率闭路连续测量系统及体温调节的生理工程研究。

基本信息

批准号：
62550294
负责人：
TAZAWA Hiroshi
金额：
$ 1.22万
依托单位：
Muroran Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

(1) A closed-circuit continuous measurement system for oxygen consumption was developed utilizing a microcomputer. The carbon dioxide produced by an embryo was simultaneously measured by detecting the changes in electrical conductivity of the CO_2 absorber which was used to eliminate produced CO_2.(2) Because the O_2 CO_2 and the heat was exchanged through the eggshell, the effects of gas conductance of the eggshell on the gas exchange, embryonic development, blood gases and hematological variables were investigated first by changing it from the beginning of incubation on for several hours.(3) The gas exchanger of embryos is the chorioallantoic membrane which is well vascularized inside the eggshell. A distribution of the cardiac output to the embryo and the chorioallantoic circulation was studied based on a circuit model analysis and a nomogram drawn with an aid of computer. The importance of blood flow in an egg's internal flows of heat was evaluated by measuring coolng rates of livi … More ng and killed chicken and quail eggs in air and in helium-oxygen mixture.(4) The heat loss from eggs when cooling was quantitatively evaluated in developing chick embryos together with heat production. Referring to these data, wa determined the cooling methods and the metabolic response of developing chick embryos were measured. It was found that a feeble homeothermic metabolic response appears in the late chick embryos and it becomes stronger after external pipping. The thyroid hormones and the eggshell gas conductance were suggested to play an important role in thermoregulation developing before hatching in chickens.(5) The metabolic response to cooling was also investigated in the semi-precocial Brown Noddy embryos at different stages of development. In contract to precocial chickens, the semi-precocial Noddy had no apparent homeothermic response before hatching.(6) In conjuction wiht studies on embryonic thermoregulation, the basic experiments were made to aim at forwarding the present study to a project elucidating the circulatory functions of developing avian embryos. Less

（1）使用微型计算机开发了一种用于氧气消耗的闭路连续测量系统。简单地通过检测CO_2吸收剂的电导率变化来测量胚胎产生的二氧化碳，用于消除产生的co_2。（2）。（2），因为O_2 co_2和热量通过蛋壳通过卵壳进行了，该蛋壳的影响是通过蛋白质的气体和embryolocy offermotic ward of embryonic变量的影响，血液的变量，血液的变量，血液的变量，血液的变量，血液的变量，血液的变量，乳液的变量，乳液的变量，乳液的变量。（3）胚胎的气体交换器是卵形膜，在蛋壳内良好的血管化。基于电路模型分析和借助计算机的戒指绘图，研究了心脏输出到胚胎和绒毛膜甲烷电路的分布。通过测量LIVI的冷却速率，评估了鸡蛋内部热流中血流的重要性……更多的NG，并在空气和氦氧混合物中杀死了鸡肉和鹌鹑蛋。（4）当冷却在发育中的胚胎中与热量产生的鸡蛋中对冷却的热量损失。在参考这些数据时，WA确定了冷却方法和发育中的雏鸡胚胎的代谢反应。发现在已故的小鸡胚胎中出现了微弱的植物热代谢反应，外部移植后它变得更强壮。建议在鸡孵化前在体温调节中发育中发挥重要作用。（5）在不同发育阶段的半预测棕色点球胚胎中还研究了对冷却的代谢反应。在与早熟鸡的合同中，半特征的Noddy在孵化前没有明显的主流反应。（6）在与胚胎热调节有关的研究中，进行了基本的实验，旨在将本研究转发到阐明开发鸟类胚胎的电路函数的项目。较少的