Experimental treatment of sarcoma by high-intensity focused ultrasound

高强度聚焦超声治疗肉瘤的实验

基本信息

批准号：
18591622
负责人：
TAZAWA Hiroshi
金额：
$ 2.34万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591622/
关键词：
HIFU Ultrasound Sarcoma Focused ultrasound High intensity focused ultrasound Ultrasoun

项目摘要

Background: Antitumor effects of HIFU (high-intensity focused ultrasound) has been investigated in several kinds of cancers, its effects on soft tissue sarcomas were rarely reported.Materials and Methods: Mouse soft tissue tumor model was made by a subcutaneous inoculation of sarcoma 180 cells (3x10^5). As the HIFU system, we used the transducer (HITACHI) and amplifier. The model tumor was treated by single shot of the HIFU, and their size, infiltration of macrophages and CD4 or CD8 positive lymphocytes, development of apoptotic cells, and mouse survival rate were observed until 6 weeks after the HIFU irradiation.Results: Size of the tumor was significantly smaller in HIFU-treated group than those in non-treated group. TRAP stain showed marked infiltration of macrophages around the remnant of the tumor cells in HIFU-treated group, but none in the non-treated group. Similarly, immunostain for CD4 or CD8 showed marked infiltration of lymphocytes in HIFU-treated group. Apoptosis surveyed by TUNEL method in the remnant tumor cells were occasionally observed in HIFU treated group, and the rate of apoptosis in HIFU-treated group was significantly higher than those in non-treated group. Survival of the sarcoma-bearing mouse in HIFU-treated group was significantly better than those in non-treated group.Conclusion: HIFU treatment for sarcoma may induced antitumor immunoresponses, even by a single shot.

背景：已经在几种癌症中研究了HIFU（高强度聚焦超声）的抗肿瘤作用，其对软组织肉瘤的影响很少被报道。材料和方法：小鼠软组织肿瘤模型是通过肉瘤下肉瘤180细胞接种的小鼠软组织肿瘤模型（3x10^5）。作为HIFU系统，我们使用了传感器（日立）和放大器。该模型肿瘤通过HIFU的单镜头进行处理，其大小，巨噬细胞的浸润，CD4或CD8阳性淋巴细胞，凋亡细胞的发育以及小鼠的存活率被观察到HIFU辐照后的6周后，肿瘤的大小相比，肿瘤的大小相比，小组中的肿瘤大小要小。陷阱染色显示在经HIFU治疗组中肿瘤细胞残留物周围的巨噬细胞明显浸润，但在未处理的组中没有。同样，用于CD4或CD8的免疫抑制剂在HIFU处理组中显示出明显的淋巴细胞浸润。在HIFU治疗组中，偶尔观察到通过TUNEL方法在残留肿瘤细胞中调查的凋亡，并且HIFU治疗组的凋亡率明显高于未经处理组的凋亡率。 HIFU治疗组中肉瘤小鼠的存活明显优于未处理组中的小鼠。判定：肉瘤的HIFU治疗也可能引起抗肿瘤免疫调查，即使是单次射击也是如此。