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Experimental treatment of sarcoma by high-intensity focused ultrasound

高强度聚焦超声治疗肉瘤的实验

基本信息

批准号：
18591622
负责人：
TAZAWA Hiroshi
金额：
$ 2.34万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591622/
关键词：
HIFU Ultrasound Sarcoma Focused ultrasound High intensity focused ultrasound Ultrasoun

项目摘要

Background: Antitumor effects of HIFU (high-intensity focused ultrasound) has been investigated in several kinds of cancers, its effects on soft tissue sarcomas were rarely reported.Materials and Methods: Mouse soft tissue tumor model was made by a subcutaneous inoculation of sarcoma 180 cells (3x10^5). As the HIFU system, we used the transducer (HITACHI) and amplifier. The model tumor was treated by single shot of the HIFU, and their size, infiltration of macrophages and CD4 or CD8 positive lymphocytes, development of apoptotic cells, and mouse survival rate were observed until 6 weeks after the HIFU irradiation.Results: Size of the tumor was significantly smaller in HIFU-treated group than those in non-treated group. TRAP stain showed marked infiltration of macrophages around the remnant of the tumor cells in HIFU-treated group, but none in the non-treated group. Similarly, immunostain for CD4 or CD8 showed marked infiltration of lymphocytes in HIFU-treated group. Apoptosis surveyed by TUNEL method in the remnant tumor cells were occasionally observed in HIFU treated group, and the rate of apoptosis in HIFU-treated group was significantly higher than those in non-treated group. Survival of the sarcoma-bearing mouse in HIFU-treated group was significantly better than those in non-treated group.Conclusion: HIFU treatment for sarcoma may induced antitumor immunoresponses, even by a single shot.

背景：HIFU（高强度聚焦超声）的抗肿瘤作用已在多种癌症中进行了研究，但其对软组织肉瘤的作用鲜有报道。材料与方法：皮下接种肉瘤180细胞（3x10^5）制作小鼠软组织肿瘤模型。作为 HIFU 系统，我们使用了换能器（HITACHI）和放大器。采用单次HIFU治疗模型肿瘤，观察其大小、巨噬细胞和CD4或CD8阳性淋巴细胞的浸润、凋亡细胞的发育情况以及小鼠存活率，直至HIFU照射后6周。结果：HIFU治疗组的肿瘤尺寸明显小于未治疗组。 TRAP染色显示HIFU治疗组肿瘤细胞残余物周围有明显的巨噬细胞浸润，但未治疗组没有明显的巨噬细胞浸润。同样，CD4 或 CD8 的免疫染色显示 HIFU 治疗组有明显的淋巴细胞浸润。 TUNEL法检测HIFU治疗组残存肿瘤细胞偶见细胞凋亡，且HIFU治疗组细胞凋亡率明显高于未治疗组。 HIFU治疗组荷瘤小鼠的存活率明显好于未治疗组。结论：HIFU治疗肉瘤即使单次注射也可诱导抗肿瘤免疫反应。