Physiological measuring system using chicken eggs as an experimental model animal for physiological functions.

以鸡蛋作为生理功能实验模型动物的生理测量系统。

• 批准号: 03452182
• 负责人: TAZAWA Hiroshi
• 金额: $ 4.29万
• 依托单位: Muroran Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03452182/
• 关键词: Experimental model animal; Egg; Embryo; Noninvasive measuring system; Ballistic movement; Instantaneous heart rate; Oxygen consumption; Altered environments; 胚質量; 貯卵; 心拍数; アロメトリー; 酸素パルス; 無侵襲計測; ベクトル心弾動; ヒヨコ; 自律神経薬; 血圧

Microcomputer systems were developed to study cardiovascular functions of avian embryos developing within the eggshell. The parameters to be measured were electrocardiogram (ECG), impedance-cardiogram (ICG), ballistocardiogram (BCG), acoustocardiogram (ACG), heart rate and arterial blood pressure of embryos and egg pressure. Measurements could be made without impeding adequate gas exchange through the eggshell and invading embryos.1. Chicken embryos (pre-pipped and externally pipped) and hatchlings were exposed to hypoxic and hyperoxic environments for 4 hours and their metabolic responses were investigated.2. Autonomic drugs were injected intravenously through a catheter, and responses of blood pressure and heart rate of chick embryos aging 12 to 18 days were studied.3. The apex-cardiogram was measured non-invasively using a piezo-electric film in newly hatched chicks and developmental patterns of heart rate during the 1-week were elucidated.4. Effects of installations of needle elect … More rodes (ECG and ICG) and microphone (ACG) upon measurements of embryonic heart rate were investigated in reference to BCG measurement.5. Tolerance of developing chick embryos to acute changes in ambient temperatures was elucidated non-invasively in reference to the heart rate.6. Two-dimensional cardiogenic ballistic movements of chicken eggs were investigated by recording trajectories of the ballistic displacement with a microcomputer system.7. Non-invasive long-term measuring system of embryonic heart rate was developed taking advantage of ACG signals and variability of the heart rate was shown during the last half of incubation.8. A new measuring system of egg BCG was developed using an electromagnetic induction coil and the BCG was recorded as a velocity of ballistic movements.9. Developmental patterns of embryonic heart rate and oxygen consumption in stored chicken eggs were investigated.10. Developmental patterns of embryonic heart rate in altricial birds, the pigeons and the bank swallow were studied. Less

利用微机系统研究了在蛋壳内发育的鸡胚的心血管功能。检测指标包括心电图（ECG）、心阻抗图（ICG）、心冲击图（BCG）、心声图（ACG）、胚胎心率、动脉血压和卵压。测量可以在不妨碍通过蛋壳和侵入胚胎的充分气体交换的情况下进行。研究结果如下：1.将鸡胚（预去核和外去核）和孵化的雏鸡暴露于低氧和高氧环境中4 h，研究其代谢反应.通过导管静脉注射自主神经药物，观察12 ~ 18日龄鸡胚的血压和心率反应.采用压电薄膜无创性测定了雏鸡心尖部心电图，揭示了雏鸡1周内心率的发育规律.针式电动机的安装效果 ...更多信息 参考BCG测量，研究了电极（ECG和ICG）和麦克风（ACG）对胚胎心率测量的影响.发育中的鸡胚对环境温度急性变化的耐受性通过参考心率非侵入性地阐明。用微机记录鸡蛋的二维心源性弹道运动轨迹，研究鸡蛋的心源性弹道运动.利用ACG信号研制了无创性的胚胎心率长期测量系统，并在孵育后期显示了心率的变异性.利用电磁感应线圈研制了一种新的鸡蛋BCG测量系统，并将BCG记录为弹道运动的速度.研究了鸡蛋贮藏过程中胚胎心率和耗氧量的发育规律。本文研究了鸟类、家鸽和家燕胚胎心率的发育规律。少

项目成果

Ono, H., Hou, P.-C.L.& Tazawa.H.: ""Responses of developing chick embryos to acute changes in ambient temperature : Noninvasive study in reference to the heart rate"" Israel Journal of Zoology. (in press). (1994)

小野 H.、侯 P.-C.L.

Tazawa, H., Watanabe, W.& Burggren, W.W.: "Embryonic heart rate in altricial birds, the pigeon (Columba domestica) and the bank swallow (Riparia riparia)" Physiological Zoology. (in press). (1994)

田泽 H.、渡边 W.

Tazawa,H.: "Non-invasive determination of heart rate in newly hatched chicks." British Poultry Science. 33. 1111-1118 (1992)

Tazawa,H.：“新孵化雏鸡心率的无创测定。”

Tazawa,H.: "Simple,noninvasive system for measuring the heart rate of avian embryos and hatchlings by means of a piezoelectric film." Medical & Biological Engineering & Computing. 31. 129-134 (1993)

Tazawa, H.：“通过压电薄膜测量鸟类胚胎和幼体心率的简单、无创系统。”

Haque, M.A., Watanabe, W., Ono, H., Sakamoto, Y.& Tazawa, H.: ""Comparisons between invasive and non-invasive determinations of embryonic heart rate in chickens"" Comparative Biochemistry & Physiology. (in press). (1994)

哈克，M.A.，渡边，W.，小野，H.，坂本，Y.