The genetic variation of parvovirus B19 in the prevalence and illness with wide spectrum of clinical manifestations

细小病毒 B19 在患病率和具有广泛临床表现的疾病中的遗传变异

• 批准号: 06670812
• 负责人: KUDOH Tooru
• 金额: $ 1.22万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670812/
• 关键词: Parvovirus; B19

Parvovirus B19 (B19) is a causative agent of erythema infectiosum. The disease is prevalent almost every 5 years. B19 infection has been known to have wide spectrum of clinical manifestations. We examined the association of B19 strains of a paticular genotype with prevalence and biological characterisrics. A genome type of the viral DNA was analyzed by digesting with restriction enzymes and assigned to 4 types according to cutting patterns. Thirty B19 strains found by dot bolt hybridization and PCR were analyzed. All the strain was from the sera obtained at the time when erythema infectiosum was prevalent. The types of B19 strains isolated from 1981 to 1982 were IV,the cutting patterns of those from 1986 to 1987 were II,the types of those from 1989 to 1990 were II and I,and the types of those from 1991 to 1992 were I,indicating a correlation between the genome type and prevalence. On the other hand any clinical manifestations from asymtomatic to bone marrow failure or disturbance of consciousenss were not associated with particular genome types in this study, suggesting that those are modified by host factors.

细小病毒 B19 (B19) 是传染性红斑的病原体。这种疾病几乎每5年流行一次。已知 B19 感染具有广泛的临床表现。我们检查了特定基因型的 B19 菌株与患病率和生物学特征的关联。通过用限制酶消化来分析病毒DNA的基因组类型，并根据切割模式将其分配为4种类型。对通过点螺栓杂交和PCR发现的30株B19菌株进行了分析。所有菌株均来自传染性红斑流行时获得的血清。 1981-1982年分离的B19毒株类型为IV型,1986-1987年分离型为II型,1989-1990年分离型为II型和I型,1991-1992年分离型为I型,表明基因组类型与流行率之间存在相关性。另一方面，从无症状到骨髓衰竭或意识障碍的任何临床表现均与本研究中的特定基因组类型无关，这表明这些临床表现受到宿主因素的影响。

项目成果

Yoto Y et al.: "Incidence of human parvovirus B19 DNA detection in blood donors" Br J Haematol. 91. 1017-1018 (1995)

Yoto Y 等人：“献血者中人类细小病毒 B19 DNA 检测的发生率”Br J Haematol。

Yoto Y et al.: "Transient disturbance of consciousness and hepatic dysfunction associated with human parvovirus B19 infection" Lancet. 344. 624-625 (1994)

Yoto Y 等人：“与人类细小病毒 B19 感染相关的短暂意识障碍和肝功能障碍”《柳叶刀》。

Yoto Y,Kudoh T,Haseyama K,Suzuki N,Chiba S,Matsunaga Y.: "Human parvovirus B19 infection in Kawasaki disease." Lancet. 344. 58-59 (1994)

Yoto Y，Kudoh T，Haseyama K，Suzuki N，Chiba S，Matsunaga Y.：“川崎病中的人类细小病毒 B19 感染。”

Yoto Y,Kudoh T,Haseyama K,Suzuki N,Matsunaga Y,Chiba S.: "Large-scale screening for human parvovirus B19 DNA in clinical specimens by dot bolt hybridization and polymerase chain reaction." J Med Virol. 47. 438-441 (1995)

Yoto Y、Kudoh T、Haseyama K、Suzuki N、Matsunaga Y、Chiba S.：“通过点螺栓杂交和聚合酶链反应大规模筛查临床样本中的人类细小病毒 B19 DNA。”

要藤裕孝 他: "ヒトパルボウイルスB19感染症のNested PCR法による診断および骨髄抑制に対するサイトカインの関与" 第42回日本ウイルス学会総会演説抄録. 131- (1994)

Hirotaka Yoto 等：“通过巢式 PCR 方法诊断人类细小病毒 B19 感染以及细胞因子参与骨髓抑制”日本病毒学会第 42 届年会摘要 131-（1994 年）。