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Wide spectrum of symptoms by parvovirus B19 infection and its relation to the virus strain

细小病毒 B19 感染的广泛症状及其与病毒株的关系

基本信息

批准号：
09670823
负责人：
KUDOH Tooru
金额：
$ 2.11万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670823/
关键词：
Parvovirus B19

项目摘要

The genetic diversity in the VP1 unique region, which is important for the neutralizing response to human parvovirus B19 was examined by the mismatch detection method using Non-isotopic RNase Cleavage Assay (NIRCAィイD1TMィエD1) and direct nucleotide sequencing. Twenty three B19 strains derived from various sources between 1986 and 1997 were examined. Three electrophoresis patterns were observed by NIRCAィイD1TMィエD1. The nucleotide sequence showed that there were 14 nucleotide changes and 4 amino acid substitutions in comparison with Au strains employed as a standard strain. The nucleotide variability of all samples ranged from 0.3% to 2.7% and the amino acid variability ranged from 1.0% to 3.0%. They were classified into three types according to NIRCAィイD1TMィエD1. Type 1 and 3 had similar sequences, but the type 2 sequence was quite different. Although there were some nucleotide variations in the same NIRCAィイD1TMィエD1 type, these were silent. However, there was no relationship between the clinical features and NIRCAィイD1TMィエD1 types or between clinical features and nucleotide sequence. All samples obtained before 1987 were NIRCAィイD1TMィエD1 type 2. On the other hand, 19 for 20 samples obtained after 1989 were NIRCAィイD1TMィエD1 type 1. The other sample obtained in 1992 was type 3. The results suggest that the B19 strain of type2 disappeared by 1988 and changed to other B19 strains such as type 1 and type 3 after 1988, indicating a correlation between genome type and prevalence. NIRCAィイD1TMィエD1 is a convenient method for screening mutations due to its simplicity and quickness.

VP1 独特区域的遗传多样性对于人类细小病毒 B19 的中和反应非常重要，通过使用非同位素 RNase 裂解测定 (NIRCAiiD1TMィD1) 的错配检测方法和直接核苷酸测序来检查。对 1986 年至 1997 年间来自不同来源的 23 个 B19 菌株进行了检查。 NIRCAィイD1TMィエD1观察到三种电泳图谱。核苷酸序列显示，与用作标准菌株的Au菌株相比，有14个核苷酸变化和4个氨基酸取代。所有样品的核苷酸变异性范围为0.3%至2.7%，氨基酸变异性范围为1.0%至3.0%。根据NIRCAィイD1TMィエD1将它们分为三种类型。 1型和3型具有相似的序列，但2型序列却有很大不同。尽管同一 NIRCAiiD1TMィエD1 类型中存在一些核苷酸变异，但这些变异是沉默的。然而，临床特征与NIRCAィイD1TMィエD1类型之间或临床特征与核苷酸序列之间没有关系。 1987年之前获得的所有样本均为NIRCAィイD1TMィエD1 2型。另一方面，1989年之后获得的20个样本中有19个为NIRCAィイD1TMィエD1 1型。1992年获得的另一个样本为3型。结果表明2型B19菌株在1988年消失并改为其他B19 1988年后的菌株如1型和3型，表明基因组类型和流行率。 NIRCAィイD1TMィエD1 因其简单快捷而成为一种便捷的突变筛查方法。