Wide spectrum of symptoms by parvovirus B19 infection and its relation to the virus strain

由于VP 1独特区域的遗传多样性，对人类淋巴结病毒B19的中性化反应很重要，使用非同位素RNase清除测定法（NIRCA-D1 TM-D1）和直接核苷酸测序方法进行了实验。23条B19线从1986年和1997年之间的各种来源中提取出来。Three electrophoresis patterns were observed by NIRCA D1 TM D1。核肽序列显示了14个核肽的变化和4个氨基酸替代物与Au strains相比的标准strains。从0.3%到2.7%，以及从1.0%到3.0%的氨基酸变异。They were classified into three types according to NIRCA D1 TM D1。1型和3型有相似的序列，但2型序列有明显不同。Although there were some nucleotide variations in the same NIRCA D1 TM D1 type， these were silent。However，临床特征和NIRCA D1 TM D1类型之间没有关系，也没有临床特征和核肽序列之间的关系。1987年前收到的所有样本都是NIRCA D1 TM D1类型2。在另一手上，1989年后收到了20个样本， 19个样本是NIRCA D1 TM D1型。1992年收到的另一个样本是第3型。1988年2型B19菌株消失的结果建议，1988年后又改变了其他B19菌株的1型和3型，表明基因组类型和显性之间的相关性。NIRCA D1 TM是一种用于screening mutations due to its simplicity and quickness的方便方法。

The genetic diversity in the VP1 unique region, which is important for the neutralizing response to human parvovirus B19 was examined by the mismatch detection method using Non-isotopic RNase Cleavage Assay (NIRCAィイD1TMィエD1) and direct nucleotide sequencing. Twenty three B19 strains derived from various sources between 1986 and 1997 were examined. Three electrophoresis patterns were observed by NIRCAィイD1TMィエD1. The nucleotide sequence showed that there were 14 nucleotide changes and 4 amino acid substitutions in comparison with Au strains employed as a standard strain. The nucleotide variability of all samples ranged from 0.3% to 2.7% and the amino acid variability ranged from 1.0% to 3.0%. They were classified into three types according to NIRCAィイD1TMィエD1. Type 1 and 3 had similar sequences, but the type 2 sequence was quite different. Although there were some nucleotide variations in the same NIRCAィイD1TMィエD1 type, these were silent. However, there was no relationship between the clinical features and NIRCAィイD1TMィエD1 types or between clinical features and nucleotide sequence. All samples obtained before 1987 were NIRCAィイD1TMィエD1 type 2. On the other hand, 19 for 20 samples obtained after 1989 were NIRCAィイD1TMィエD1 type 1. The other sample obtained in 1992 was type 3. The results suggest that the B19 strain of type2 disappeared by 1988 and changed to other B19 strains such as type 1 and type 3 after 1988, indicating a correlation between genome type and prevalence. NIRCAィイD1TMィエD1 is a convenient method for screening mutations due to its simplicity and quickness.