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DIELECTRIC SPECTROGRAM FOR EVALUATION OF ISCHEMIC INJURY OF CIRRHOTIC LIVER

评估肝硬化缺血性损伤的介电谱图

基本信息

批准号：
06671260
负责人：
SAKAI Satoshi
金额：
$ 1.15万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671260/
关键词：
Electrical impedance Hepatic ischemia Normothermic ischemia of the liver Cold ischemia of the liver Liver preservation High energy phophate Fatty liver Liver cirrhosis ミトコンドリアスコア UW液肝虚血再灌流障害胆汁流量脂肪肝肝硬変

项目摘要

1.We measured electrical impedance of the liver in experimental nornmothermic hepatic ischemia-reperfusion period. Resistivity (rho) increased immediately after the initiation of ischemia and showed the maximum value (rho1) about 40 minutes after ischemia and slowly decreased thereafter. rho2 was the value just before the reperfusion. There was significant correlation ship between rho2/rho1 and the recovery rate of bile flow 60 minutes after the reperfusion. This result suggested that rho2/rho1 would be an reliable indices predict the viability of the warm-ischemically injured liver of the rat.2.Changes in rho of the choline deficient diet induced fatty liver during ischemia-reperfusion period was slighter than those of healthy liver.3.In the clinical hepatic resection, rho of the healthy liver showed rapid increased after hepatic ischemia until 50 minutes and slowly decreased thereafter. These changes were the same manner as those of healthy rat liver in the experiment. On the other hand, changes in rho of the fatty liver or cirrhotic liver was slighter than those of healthy liver. These data suggested that slight changes in rho during ischemia should indicate that livers were not healthy.4.In the experiment of cold ischemia of the healthy rat liver, % tan delta, which was the ratio to loss tan delta at the beginning of ischemia, decreased until four hours and 12 hours after ischemia in saline and UW solution, respectively. There was significant correlation ship between % tan delta and % ATP, which is the ratio to the ATP just before cold ischemia. These suggested that loss tan delta would indicate the ATP level of the liver until four hours and 12 hours after cold ischemia, in cold saline and in cold UW solution. Loss tan delta did not affected by the water contents in the liver.

1.我们测量了实验正常肝缺血再灌注期肝脏的电阻抗。电阻率（rho）在缺血开始后立即升高，在缺血约40分钟后达到最大值（rho1），此后缓慢下降。Rho2为再灌注前的值。再灌注后60分钟，rho2/rho1与胆汁流量恢复率有显著相关性。结果表明，rho2/rho1可作为预测大鼠热缺血损伤后肝脏活力的可靠指标。缺乏胆碱饮食引起的脂肪肝缺血-再灌注期rho的变化较健康肝轻微。在临床肝切除术中，正常肝脏rho在肝缺血50分钟后迅速升高，之后缓慢下降。这些变化与实验中健康大鼠肝脏的变化相同。另一方面，脂肪肝和肝硬化的rho变化较健康肝轻微。这些数据表明，缺血期间rho的轻微变化可能表明肝脏不健康。在健康大鼠肝脏冷缺血实验中，在生理盐水和UW溶液中，% tan δ与缺血开始时的损失tan δ之比分别下降至缺血后4小时和12小时。% tan δ与% ATP（与冷缺血前的ATP之比）之间存在显著相关。这表明，在冷缺血后4小时和12小时，在冷生理盐水和冷UW溶液中，tan δ的损失可以指示肝脏ATP水平。损失tan δ不受肝脏含水量的影响。