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Molecular Mechanism and Engineering of Sequential Reactions in Natural Products Biosynthesis in Plants

Molecular Mechanism and Engineering of Sequential Reactions in Natural Products Biosynthesis in Plants
植物天然产物生物合成中顺序反应的分子机制和工程
批准号：
06680552
负责人：
SAITO Kazuki
金额：
$1.47万
依托单位：
CHIBA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
项目状态：
已结题
起止时间：
1994 至 1995
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680552/
关键词：
Genetic engineeringNatural productsMoleculart cloningBiosynthesisAmino acidsSulfur metabolism転写因子アントシアニン

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中文摘要
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英文摘要
The non-protein amino acids represent one of the major groups in secondary metabolites in plants. These compounds were synthesized via sequential reactions catalyzed by specific enzymes. The cDNA encoding b-PA/DSase was isolated from an expression cDNA library of watermelon seedlings by genetic conmplementation in Cys-E.coli lacking CSase loci. The expressed protein by the clone exhibited the activity of b-PA synthesis, indicating the identity of the clone encoding b-PA/CSase. Over-expression system of b-PA/CSase in E.coli was established by using a T7 promoter-based vector. The recombinant b-PA/CSase from watermelon was able to synthesize mimosine and quisqualic acid as well as b-PA in vitro. b-PA was also formed by in vivo experiments fed with pyrazole and serine as the biosynthetic precursors. The cDNA clone of SATase was isolated from watermelon and over-expressed in E.coli. Biochemical characterization has been performed by using the purified recombinant protein.
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M.Noji,他: "Molecular cloning of a cystein synthase cDNA from Citrullus vulgaris (watermelon) by genetic complementation in an Escherichia coli Cysauxotroph." Mol.Gen.Genet.244. 57-66 (1994)
M.Noji 等人：“通过大肠杆菌 Cysauxotropic 中的遗​​传互补对西瓜中的半胱氨酸合酶 cDNA 进行分子克隆”（Mol.Gen.Genet.244）。
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通讯作者：
Noji M,Murakoshi I,Saito K: "Molecular cloning of a cysteine synthase cDNA from Citrullus vulgaris (watermelon) by genetic complementation in an Escherichia coli Cys-auxotroph." Mol. Gen. Genet. 244. 57-66 (1994)
Noji M、Murakoshi I、Saito K：“通过大肠杆菌 Cys 营养缺陷型基因互补，从西瓜中分子克隆半胱氨酸合酶 cDNA。”
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通讯作者：
Saito K,Kanda R,Kurosawa M,Murakoshi I: "Overexpression of a plant cysteine synthase gene and biosynthesis of a plant specific metabolite, b- (pyrazol-1-y1) -L-alanine, in Escherichia coli." Can. J.Chem. 72. 188-192 (1994)
Saito K、Kanda R、Kurosawa M、Murakoshi I：“在大肠杆菌中植物半胱氨酸合酶基因的过度表达和植物特异性代谢物 b-(pyrazol-1-y1)-L-丙氨酸的生物合成。”
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M.Noji,他: "Evidence for identity of b-pyrazolealanine synthase with cystein stnthase in watermelon : Formation of b-pyrazolealanine cloned cystein synthase in vitro and in vivo." Biochem.Biophys.Res.Commum.197. 1111-1117 (1993)
M. Noji 等人：“西瓜中 b-吡唑丙氨酸合酶与半胱氨酸合成酶同一性的证据：体外和体内 b-吡唑丙氨酸克隆半胱氨酸合成酶的形成。”1111-。 1117 (1993)
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共 10 条
    Establishment of molecular basis on ligand recognition by the insect prothoracicotropic hormone receptor
    Systematic structure optimization of the EGF receptor-inhibiting peptide through use of a screening technology
    • 批准号：
      24590134
    • 项目类别：
      Grant-in-Aid for Scientific Research (C)
    • 资助金额：
      $3.49万
    • 财政年份：
      2012
    • 负责人：
      SAITO Kazuki
    • 依托单位：
      The University of Tokyo
    Integrated analysis of transcriptome and metabolome in Japanese medicinal plants
    • 批准号：
      24390030
    • 项目类别：
      Grant-in-Aid for Scientific Research (B)
    • 资助金额：
      $11.65万
    • 财政年份：
      2012
    • 负责人：
      SAITO Kazuki
    • 依托单位：
      Chiba University
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